Benchtop NMR spectrometers in academic teaching

Riegel, Susanne D.; Leskowitz, Garett M.

doi:10.1016/j.trac.2016.01.001

Cited by 51 publications

(43 citation statements)

References 60 publications

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“…It is worth emphasizing some important strengths of metabolomic analysis by 1 H-NMR: (i) it is an intrinsically quantitative technique, which can avoid relying on internal standards [ 55 ], (ii) the experimental protocol is therefore extremely simple and quick, allowing processing tens of samples per batch, (iii) the method for sample preparation is the same used for the ribosomal sequences analysis, allowing excellent integration of the two techniques, (iv) the cost per analysis, at present slightly lower than gene sequencing, can be foreseen to drop dramatically in the short term [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Novel approaches for the taxonomic and metabolic characterization of lactobacilli: Integration of 16S rRNA gene sequencing with MALDI-TOF MS and 1H-NMR

et al. 2017

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Lactobacilli represent a wide range of bacterial species with several implications for the human host. They play a crucial role in maintaining the ecological equilibrium of different biological niches and are essential for fermented food production and probiotic formulation. Despite the consensus about the ‘health-promoting’ significance of Lactobacillus genus, its genotypic and phenotypic characterization still poses several difficulties. The aim of this study was to assess the integration of different approaches, genotypic (16S rRNA gene sequencing), proteomic (MALDI-TOF MS) and metabolomic (1H-NMR), for the taxonomic and metabolic characterization of Lactobacillus species. For this purpose we analyzed 40 strains of various origin (intestinal, vaginal, food, probiotics), belonging to different species. The high discriminatory power of MALDI-TOF for species identification was underlined by the excellent agreement with the genotypic analysis. Indeed, MALDI-TOF allowed to correctly identify 39 out of 40 Lactobacillus strains at the species level, with an overall concordance of 97.5%. In the perspective to simplify the MALDI TOF sample preparation, especially for routine practice, we demonstrated the perfect agreement of the colony-picking from agar plates with the protein extraction protocol. 1H-NMR analysis, applied to both culture supernatants and bacterial lysates, identified a panel of metabolites whose variations in concentration were associated with the taxonomy, but also revealed a high intra-species variability that did not allow a species-level identification. Therefore, despite not suitable for mere taxonomic purposes, metabolomics can be useful to correlate particular biological activities with taxonomy and to understand the mechanisms related to the antimicrobial effect shown by some Lactobacillus species.

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Section: Discussionmentioning

confidence: 99%

Novel approaches for the taxonomic and metabolic characterization of lactobacilli: Integration of 16S rRNA gene sequencing with MALDI-TOF MS and 1H-NMR

et al. 2017

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“…In addition to classic spectroscopy and relaxometry measurements, some benchtop systems also allow the investigation of mass transport phenomena by PFG NMR. Besides their value for teaching purposes [18][19][20], they have been widely utilised for in-and on-line measurements of homo-and heterogeneous reactions in flow and batch systems [21][22][23][24][25] and fermentation processes [26]. In contrast to the previously described high field MRI studies, reports that utilised a benchtop NMR instrument to inves-tigate heterogeneous reactions, only used it as an ex situ sampling technique.…”

Section: Introductionmentioning

confidence: 99%

In situ reaction monitoring in heterogeneous catalysts by a benchtop NMR spectrometer

Leutzsch¹,

Sederman²,

Gladden³

et al. 2019

Magnetic Resonance Imaging

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Understanding the reactivity and mass transport properties of porous heterogenous catalysts is important for the development of new materials. Whereas MRI has previously been used to correlate chemical kinetics and hydrodynamics under operando conditions, this paper demonstrates that a modern benchtop NMR spectrometer is a suitable alternative to obtain diverse reaction information in porous heterogeneous catalyst materials on a smaller scale. Besides information about the chemical conversion within the pores, it can also be used to study changes of surface interaction by T 1 /T 2 NMR relaxometry techniques and changes in mass transport by PFG NMR from a single chemical reaction.

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“…Aktuelle Tisch‐NMR‐Spektrometer eignen sich zur Messung unterschiedlicher Atomkerne, wie 1 H, 15 N, 19 F, 29 Si, 31 P, entweder als Ein‐ oder Zweikanalgeräte. Sie finden ihre Anwendung in der akademischen und industriellen Forschung bei der Untersuchung kleiner Moleküle sowie in der Lehre . Vor der kommerziellen Einführung kompakter NMR‐Spektrometer wurde die potenzielle Nutzung von Tisch‐Spektrometern von einigen Pionieren auf diesem Gebiet für die chemische Analyse, Reaktionsüberwachung und Prozesskontrolle ergründet .…”

Section: Fourier‐nmrunclassified

NMR mit Tischgeräten und deren Anwendungen von der Materialwissenschaft bis zur organischen Chemie

Blümich

Singh

2017

Angewandte Chemie

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Die NMR‐Spektroskopie ist eine unverzichtbare Analysemethode. Bis vor kurzem erforderten die Geräte viel Platz, waren teuer und schwer zu bedienen. Dies hat sich mit der Einführung kompakter NMR‐Spektrometer geändert, die sich für die Analyse kleiner Moleküle auf der Laborbank eignen. Solche Spektrometer sind mit Permanentmagneten für 1H‐NMR‐Frequenzen zwischen 40 und 80 MHz ausgestattet. Erst seit wenigen Jahren gibt es kleine Permanentmagnete mit homogenen Magnetfeldern für hochaufgelöste Spektroskopie. Tischgeräte mit inhomogenen Feldern werden schon seit 40 Jahren zur Charakterisierung von Lebensmitteln und Materialien über Relaxations‐ und Diffusionsmessungen genutzt. Ähnliche NMR‐Geräte messen diese Parameter im Streufeld außerhalb des Magneten. Sie werden zur Untersuchung von Bohrlochwänden in Ölquellen sowie für zerstörungsfreie Analysenverwendet. Der gegenwärtige Stand von NMR‐Spektroskopie, ‐Bildgebung und ‐Relaxometrie wird hier anhand repräsentativer Beispiele beschrieben.

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Benchtop NMR spectrometers in academic teaching

Cited by 51 publications

References 60 publications

Novel approaches for the taxonomic and metabolic characterization of lactobacilli: Integration of 16S rRNA gene sequencing with MALDI-TOF MS and 1H-NMR

Novel approaches for the taxonomic and metabolic characterization of lactobacilli: Integration of 16S rRNA gene sequencing with MALDI-TOF MS and 1H-NMR

In situ reaction monitoring in heterogeneous catalysts by a benchtop NMR spectrometer

NMR mit Tischgeräten und deren Anwendungen von der Materialwissenschaft bis zur organischen Chemie

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