2020
DOI: 10.1038/s41598-020-61561-7
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Benchtop-fabricated lipid-based electrochemical sensing platform for the detection of membrane disrupting agents

Abstract: there are increasing concerns about the danger that water-borne pathogens and pollutants pose to the public. of particular importance are those that disrupt the plasma membrane, since loss of membrane integrity can lead to cell death. currently, quantitative assays to detect membrane-disrupting (lytic) agents are done offsite, leading to long turnaround times and high costs, while existing colorimetric point-of-need solutions often sacrifice sensitivity. Thus, portable and highly sensitive solutions are needed… Show more

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Cited by 13 publications
(9 citation statements)
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References 43 publications
(30 reference statements)
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“…The wrinkled gold surface has been extensively investigated in previous publications from our group and others. 31,32,[35][36][37][38][39] To determine the functionality and affinity of the aptamers in the E-AB format, all initial experiments were first performed on commercial disc (CD) Au electrodes (CH Instruments, 2 mm diameter) in a standard 3-electrode electrochemical cell ( Figure 2A). The commercial gold electrodes were functionalized with the aptamers and backfilled with blocking MCH monolayers.…”
Section: Resultsmentioning
confidence: 99%
“…The wrinkled gold surface has been extensively investigated in previous publications from our group and others. 31,32,[35][36][37][38][39] To determine the functionality and affinity of the aptamers in the E-AB format, all initial experiments were first performed on commercial disc (CD) Au electrodes (CH Instruments, 2 mm diameter) in a standard 3-electrode electrochemical cell ( Figure 2A). The commercial gold electrodes were functionalized with the aptamers and backfilled with blocking MCH monolayers.…”
Section: Resultsmentioning
confidence: 99%
“…In this sense, high surface area miniaturized electrodes have become increasingly attractive for applications in biosensing, electronic, and lab‐on‐a‐chip devices. [ 1–5 ] Several approaches have been used to increase the electroactive surface area (ESA) of electrodes used for electrochemical sensing, with the majority of them focused on introducing roughness onto the electrode surface. [ 6 ] A recently developed method to increase the surface area uses the shrinking and wrinkling of a thin film, which produces miniaturized micro‐structured electrodes (MSE).…”
Section: Introductionmentioning
confidence: 99%
“…We have evaluated gold film thicknesses ranging from 2 to 400 nm, an observed that all yielded 6-7-fold increases in ESA, when compared with planar electrodes of the same footprint. [21][22][23]. Here, we demonstrate that the ESA of gold MSEs fabricated through thermal shrinking and wrinkling can be further enhanced using a simple and cost-effective chronoamperometric pulsing technique.…”
Section: Introductionmentioning
confidence: 99%