2024
DOI: 10.3897/mbmg.8.128646
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Benchmarking the discrimination power of commonly used markers and amplicons in marine fish (e)DNA (meta)barcoding

João T. Fontes,
Kazutaka Katoh,
Rui Pires
et al.

Abstract: Environmental DNA (eDNA) metabarcoding is revolutionising the study of aquatic ecosystems, enabling high-throughput analysis of biodiversity with minimal disturbance. Despite its potential to support fisheries management, species identification and downstream analysis reliability are hindered by the lack of standardisation in DNA fragment choice. This study compares the species discrimination power of three markers used in marine fish (e)DNA (meta)barcoding – 12S rRNA, 16S rRNA and cytochrome oxidase subunit I… Show more

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