“…This approach works primarily by disintegrating bacterial cell walls. A variety of methods have been used in the literature to achieve this aim including: the addition of anoxic/anaerobic stages (Barker & Dold, 1996;Yoon, 2003), enzyme treatment (Barjenbruch & Kopplow, 2003), freezing and thawing (Chu et al, 1999), the use of an inclined-plate membrane bioreactor (Xing et al, 2006), ozonation (Yasui et al, 1996;Sakai et al, 1997;Egemen et al, 2001;Song et al, 2003;Wang et al, 2008), ozonation combined with chemical treatment (Oh el al, 2007), thermal treatment (Goma et al, 1997;Kepp et al, 1999), and ultrasound (Yoon et al, 2004b). Combinations of these treatments are also possible.…”