Bench-scale Overproduction and Purification of recombinant GCSF in Escherichia coli fed-batch process
Abstract:Fermentation process scale up can change the hydrodynamic conditions of recombinant E. coli cultivation bioreactor and reduce productivity in the same operating conditions that have been developed at lab scale. In this study, an efficient strategy was used to scale up recombinant human GCSF production in the fed -batch culture of E. coli. Heterologous production of human GCSF was done in the fed-batch culture of Escherichia coli BL21 (DE3) with feeding strategy of maximum attainable specific growth rate. Then,… Show more
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