Bence-Jones Proteinemia in Multiple Myeloma 1

Occurrence of Double Monoclonal Bands on Protein Electrophoresis: An Unusual Finding

“…Longsworth et al [2] were the first to demonstrate a tall, narrow based spike by serum protein electrophoresis (SPEP) which was later termed as ''M'' component/peak by Moore et al [3]. On performing serum protein electrophoresis, monoclonal Igs appear as single intense, discrete band on agarose gel and a sharp peak in the densitometer tracing.…”

Multiple Myeloma. Iii. Effect of Urethane Therapy on Plasma Cell Growth, Abnormal Serum Protein Components and Bence Jones Proteinuria 1

“…The numerous important studies (7,11,15,(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) during recent years of the serum and urinary proteins of multiple myeloma utilizing neutral salt precipitation, electrophoresis, ultracentrifugation, and immunochemical methods have been admirably reviewed by Gutman (30). Bence Jones protein is mainly characterized by its almost exclusive occurrence in malignancies involving the hemopoietic or lymphoid tissues (6) and by its peculiar coagulation reactions when heated.…”

“…Electrophoretic studies in many instances have shown remarkably homogenous proteins but the mobility of specimens obtained from different individuals ranges from gamma to that of beta globulin. When studied immunologically, specific precipitins of at least two antigenic types have been found (12,27,31). The amino acid composition has been reinvestigated recently by improved methods (32)(33)(34).…”

“…Many sera contain large peaks of electrophoretically homogenous protein with mobilities that range in different instances from slower than the mobility of gamma to that of beta globulin. These are usually associated with decreased albumin and in some instances with moderate increases in the alpha globulins (17,19,21,24,27,35,36). Patterns with large peaks in the beta region or intermediate between beta and gammna are virtually diagnostic of myeloma (27).…”

“…The electrophoretic mobilities of the abnormal serum components have generally been somewhat less than that of the urinary protein from the same individual. Large serum protein increments, especially those with the mobility of beta globulin or intermediate between beta and gamma have been shown in some instances to be due to Bence Jones proteinemia (21,27). In other instances, especially where peaks with gamma mobility occur, the protein in the serum has been of much larger molecular size, differing in solubility and even in antigenic property (27).…”