Belastbare Enzympräparate für die technische Biokatalyse

Ansorge‐Schumacher, Marion B.; Nieguth, René; Wiemann, Lars O.; Weißhaupt, P.; Thum, Oliver; Eckstein, Marrit

doi:10.1002/cite.200900105

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…Since the mixture is very viscous, high energy input by the mixture is necessary, which influences the integrity of the Novozym 435. Recent work from Ansorge-Schumacher et al suggests that this might be a major problem . The diameter of Novozym 435 beads is in the range of 300−900 μm .…”

Section: Discussionmentioning

confidence: 99%

Scaleup of Lipase-Catalyzed Polyester Synthesis

Korupp

Weberskirch

Müller

et al. 2010

Org. Process Res. Dev.

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One of the critical steps for the commercialization of new enzymebased products is the successful scaleup of the catalyzed reaction. In the study presented here, we achieved a scaleup for the enzymatic production of glycerol adipate on a 500 g scale in a heated, solvent-free system. The influence of various reaction conditions (i.e., temperature, pressure, enzyme concentration, reactants ratio, stirrer type, stirring rate, and reaction time) on the substrate conversion and molecular weight of the product was investigated. Conversions were higher than 0.9, and molecular weights were in the desired range of 2000-3000 Da. Space time yields of 370 g d -1 L -1 could be achieved. Maximal polymer yield was achieved at 60 °C, < 20 mbar, 3 wt % Novozym 435, glycerol:adipic acid ratio 1.1:1, <48 h while stirring at 100 rpm.

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Section: Discussionmentioning

confidence: 99%

Scaleup of Lipase-Catalyzed Polyester Synthesis

Korupp

Weberskirch

Müller

et al. 2010

Org. Process Res. Dev.

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“…The fixed bed reactor approach was chosen for two main reasons: (i) easy and efficient enzyme recycling due to a permanent separation of product and catalyst and (ii) avoiding unnecessary mechanical stress to the immobilized enzyme (as in the case of stirring). The latter effect has already been described in several studies using batch stirred tank reactors (STRs). , The mechanical fragility of the solid support (methacrylate/divinylbenzene) is often the main reason for limited catalyst lifetime when used in STRs . Furthermore, abrasion and grinding of the carrier results in the production of fine powder, causing problems with blocking of filters when applied on a large scale .…”

Section: Resultsmentioning

confidence: 98%

“…The latter effect has already been described in several studies using batch stirred tank reactors (STRs). 18,19 The mechanical fragility of the solid support (methacrylate/divinylbenzene) is often the main reason for limited catalyst lifetime when used in STRs. 20 Furthermore, abrasion and grinding of the carrier results in the production of fine powder, causing problems with blocking of filters when applied on a large scale.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Process Development for the Sustainable and Economic Production of Biobased and Biodegradable High-Performance Lubricants

Guntermann,

Koch,

Gröger

2024

ACS Sustainable Chem. Eng.

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In this contribution, we present a process development for an easy, efficient, and sustainable synthesis of recently developed biobased and biodegradable high-performance lubricants utilizing lipase catalysis. The lubricants are produced starting from a regioisomeric mixture of methyl 9-/10-hydroxymethylstearate as an oleic acid-derived raw material by means of solventfree esterification at low temperatures, making them highly attractive for industrial-scale applications. The process setup is semicontinuous and enables a simple and efficient catalyst recycling, which has been demonstrated over in total 21 reaction cycles. The immobilized lipase catalyst (Novozym 435) shows excellent long-term stability within the process with a very low loss of enzyme activity of only 0.25% per cycle, thus opening up the perspective for an economically feasible production on an industrial scale. Furthermore, an E factor of <0.15 was calculated, exemplified for the synthesis of a moderately viscous oil.

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“…A similar observation was made in our case using a 3-bladed spiral propeller instead of a 4-bladed flat turbine (unpublished data), suggesting that homogeneity of highly viscous mixtures can be reached optimally in an STR with stirrers that induce vertical convection due to a rather axial flow. Indeed, studies from Wiemann et al and Ansorge-Schumacher et al are suggesting that viscous mixtures requiring high energy input might have a deleterious effect on the physical and mechanical properties of immobilized formulations [ 71 , 72 ]. To corroborate these affirmations, a study from Keng et al used a Rushton turbine impeller, providing this time a radial flow that was seemingly more adapted to the relatively lower viscosities of their n -hexane-based mixture [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Lipase-Catalyzed Production of Sorbitol Laurate in a “2-in-1” Deep Eutectic System: Factors Affecting the Synthesis and Scalability

et al. 2021

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Surfactants, such as glycolipids, are specialty compounds that can be encountered daily in cleaning agents, pharmaceuticals or even in food. Due to their wide range of applications and, more notably, their presence in hygiene products, the demand is continuously increasing worldwide. The established chemical synthesis of glycolipids presents several disadvantages, such as lack of specificity and selectivity. Moreover, the solubility of polyols, such as sugars or sugar alcohols, in organic solvents is rather low. The enzymatic synthesis of these compounds is, however, possible in nearly water-free media using inexpensive and renewable building blocks. Using lipases, ester formation can be achieved under mild conditions. We propose, herein, a “2-in-1” system that overcomes solubility problems, as a Deep Eutectic System (DES) made of sorbitol and choline chloride replaces either a purely organic or aqueous medium. For the first time, 16 commercially available lipase formulations were compared, and the factors affecting the conversion were investigated to optimize this process, owing to a newly developed High-Performance Liquid Chromatography-Evaporative Light Scattering Detector (HPLC-ELSD) method for quantification. Thus, using 50 g/L of lipase formulation Novozym 435® at 50 °C, the optimized synthesis of sorbitol laurate (SL) allowed to achieve 28% molar conversion of 0.5 M of vinyl laurate to its sugar alcohol monoester when the DES contained 5 wt.% water. After 48h, the de novo synthesized glycolipid was separated from the media by liquid–liquid extraction, purified by flash-chromatography and characterized thoroughly by one- and two-dimensional Nuclear Magnetic Resonance (NMR) experiments combined to Mass Spectrometry (MS). In completion, we provide initial proof of scalability for this process. Using a 2.5 L stirred tank reactor (STR) allowed a batch production reaching 25 g/L in a highly viscous two-phase system.

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Belastbare Enzympräparate für die technische Biokatalyse

Cited by 7 publications

References 17 publications

Scaleup of Lipase-Catalyzed Polyester Synthesis

Scaleup of Lipase-Catalyzed Polyester Synthesis

Process Development for the Sustainable and Economic Production of Biobased and Biodegradable High-Performance Lubricants

Lipase-Catalyzed Production of Sorbitol Laurate in a “2-in-1” Deep Eutectic System: Factors Affecting the Synthesis and Scalability

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