BEL1-like protein (StBEL5) regulates CYCLING DOF FACTOR1 (StCDF1) through tandem TGAC core motifs in potato

Kondhare, Kirtikumar R.; Vetal, Pallavi Vijay; Kalsi, Harpreet S; Banerjee, Anjan K.

doi:10.1016/j.jplph.2019.153014

Cited by 21 publications

(17 citation statements)

References 46 publications

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“…During different stolon-to-tuber transitional stages, the transcript levels of StBEL5 and StCDF1 were significantly higher under SD photoperiodic conditions compared to LD (Kondhare et al, 2019). GUS activity was also seen in the tuber pith, tuber-stalk junction and 3-months old tuber sprouts of StCDF1::GUS promoter transgenic lines, suggesting that StBEL5-StCDF1 module is functional during tuber development (Kondhare et al, 2019). These results establish that StBEL5 functions upstream to StCDF1 gene in potato.…”

Section: The Phloem Mobile Ft Protein Stsp6a Forms the Tuberization Activation Complex (Tac) In The Stolon To Regulate Tuber Initiationsupporting

confidence: 52%

“…Using transgenic potato lines harboring the wild-type or the TGAC mutated promoter StCDF1 fused to a reporter gene GUS, we showed that wild-type promoter StCDF1::GUS transgenic potato lines exhibit a widespread GUS activity, whereas mutated promoter StCDF1::GUS transgenic lines exhibited a complete abolishment of GUS activity. Moreover, yeast one-hybrid assay showed that St-BEL5 protein interacts with the wild-type StCDF1 promoter, but not with the TGAC mutated StCDF1 promoter (Kondhare et al, 2019). During different stolon-to-tuber transitional stages, the transcript levels of StBEL5 and StCDF1 were significantly higher under SD photoperiodic conditions compared to LD (Kondhare et al, 2019).…”

Section: The Phloem Mobile Ft Protein Stsp6a Forms the Tuberization Activation Complex (Tac) In The Stolon To Regulate Tuber Initiationmentioning

confidence: 99%

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Molecular signals that govern tuber development in potato

Kondhare¹,

Natarajan²,

Banerjee³

2020

Int. J. Dev. Biol.

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The potato serves as the fourth most important food crop on the planet after the three cereal crops. It is rich in starch, storage proteins and important vitamins, dietary antioxidants and minerals. Potato is a modified stem (stolon) that grows underground, at the base of the plant, under favourable conditions. Perception and processing of signals occur in leaves and the corresponding information is transported to the stolon-tip. The elongation of the stolon-tip ceases and the plane of cell division changes from transverse to longitudinal, causing swelling of the sub-apical region of the stolon. This is accompanied by synthesis of starch in leaves, followed by its transport to and accumulation in the stolon. The initiation of tuber developmental signals and the subsequent stolon-to-tuber transition (tuberization) is undoubtedly a dynamic process which involves integration of multiple molecular factors, environmental cues and crosstalk between various pathways, including phytohormones. Understanding the tuberization process has been an aim of many plant biologists across the globe. Recent discoveries have shown that apart from photoperiod and hormonal metabolism, there are crucial transcription factors, small RNAs, full-length mobile mRNAs and proteins that regulate tuberization in potato. Although we have gained significant knowledge about the tuberization process, many questions on the underlying mechanisms of tuber development remain to be answered. In this review, we summarize the crucial molecular signals that govern tuber formation and propose an updated tuberization network along with future research directions.

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Section: The Phloem Mobile Ft Protein Stsp6a Forms the Tuberization Activation Complex (Tac) In The Stolon To Regulate Tuber Initiationsupporting

confidence: 52%

Section: The Phloem Mobile Ft Protein Stsp6a Forms the Tuberization Activation Complex (Tac) In The Stolon To Regulate Tuber Initiationmentioning

confidence: 99%

Molecular signals that govern tuber development in potato

Kondhare¹,

Natarajan²,

Banerjee³

2020

Int. J. Dev. Biol.

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“…We noted from an analysis of PGSC RNA-Seq data that the StCDF4 locus also has a secondary gene model that is very similar to that of StCDF1, indicating the possible presence of an lncRNA transcript for this locus (PGSC, 2011). Recently, Kondhare et al (2019) showed that StCDF1 gene expression is regulated by a BEL1-like protein (StBEL5). Interestingly, we found that the KNOX-partner of StBEL5 (StPOTH1) is downregulated in 35S:StCDF1 plants, indicating complex upstream regulatory circuitry that will require further analysis.…”

Section: Discussionmentioning

confidence: 99%

Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response

Gonzales

Bergonzi

et al. 2021

The Plant Journal

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Plants regulate their reproductive cycles under the influence of environmental cues, such as day length, temperature and water availability. In Solanum tuberosum (potato), vegetative reproduction via tuberization is known to be regulated by photoperiod, in a very similar way to flowering. The central clock output transcription factor CYCLING DOF FACTOR 1 (StCDF1) was shown to regulate tuberization. We now show that StCDF1, together with a long non-coding RNA (lncRNA) counterpart, named StFLORE, also regulates water loss through affecting stomatal growth and diurnal opening. Both natural and CRISPR-Cas9 mutations in the StFLORE transcript produce plants with increased sensitivity to water-limiting conditions. Conversely, elevated expression of StFLORE, both by the overexpression of StFLORE or by the downregulation of StCDF1, results in an increased tolerance to drought through reducing water loss. Although StFLORE appears to act as a natural antisense transcript, it is in turn regulated by the StCDF1 transcription factor. We further show that StCDF1 is a non-redundant regulator of tuberization that affects the expression of two other members of the potato StCDF gene family, as well as StCO genes, through binding to a canonical sequence motif. Taken together, we demonstrate that the StCDF1-StFLORE locus is important for vegetative reproduction and water homeostasis, both of which are important traits for potato plant breeding.

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“…In addition to being translocated, it was postulated that StBEL5 and POTH1 mRNA is translated in leaves, where they together activate expression of StSP6A and StCDF1 by binding to specific elements in their promoters (72,73,122). In leaves, BEL5 expression was also proposed to be regulated by PHYB via upregulation of miR172 and its target, RELATED TO APETALA2 1 (RAP1) (88).…”

Section: Mobile Rnas Contribute To Storage Organ Formationmentioning

confidence: 99%

Tuber and Tuberous Root Development

Zierer

Rüscher

Sonnewald

et al. 2021

Annu. Rev. Plant Biol.

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Root and tuber crops have been an important part of human nutrition since the early days of humanity, providing us with essential carbohydrates, proteins, and vitamins. Today, they are especially important in tropical and subtropical regions of the world, where they help to feed an ever-growing population. Early induction and storage organ size are important agricultural traits, as they determine yield over time. During potato tuberization, environmental and metabolic status are sensed, ensuring proper timing of tuberization mediated by phloem-mobile signals. Coordinated cellular restructuring and expansion growth, as well as controlled storage metabolism in the tuber, are executed. This review summarizes our current understanding of potato tuber development and highlights similarities and differences to important tuberous root crop species like sweetpotato and cassava. Finally, we point out knowledge gaps that need to be filled before a complete picture of storage organ development can emerge. Expected final online publication date for the Annual Review of Plant Biology, Volume 72 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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BEL1-like protein (StBEL5) regulates CYCLING DOF FACTOR1 (StCDF1) through tandem TGAC core motifs in potato

Cited by 21 publications

References 46 publications

Molecular signals that govern tuber development in potato

Molecular signals that govern tuber development in potato

Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response

Tuber and Tuberous Root Development

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