Beitrag zum Problem der Fluoreszenzlöschung Vergleichende Untersuchung der Löschwirkung von Halogensalzen auf die Fluoreszenz von Chinolin und verschiedenen Chinolin‐Dervaten in saurer Lösung

Eisenbrand, J.; Raisch, M.

doi:10.1002/food.19620060207

Cited by 5 publications

(1 citation statement)

References 6 publications

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“…[1][2][3][4][5][6][7][8][9][10][11] The quenching of fluorescence was typically used for metal cations, but the ability of halide ions to quench fluorescence has also been reported to involve quinine sulfate 1,2 and other dyes. [3][4][5][6][7] Fluorescence quenching has been utilized for the detection of non-fluorescent species in various separation methods.…”

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confidence: 99%

Indirect Detection of Halide Ions via Fluorescence Quenching of Quinine Sulfate in Microcolumn Ion Chromatography

Takeuchi

Sumida

2004

ANAL. SCI.

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Quenching of fluorescence is a well-known physicochemical phenomenon that has been utilized for the selective determination of quenching species. 1-11The quenching of fluorescence was typically used for metal cations, but the ability of halide ions to quench fluorescence has also been reported to involve quinine sulfate 1,2 and other dyes. [3][4][5][6][7] Fluorescence quenching has been utilized for the detection of non-fluorescent species in various separation methods. [8][9][10][11] Amankwa and Kuhr 8 accomplished indirect fluorimetric detection using quinine sulfate as the visualization agent in micellar electrokinetic chromatography. They found that the detection mechanism involved a combination of displacement of the fluorophore from the micelle by the analytes and net reduction of the quantum efficiency of the fluorophore in the sample zone. Smith and El Rassi 9 also employed N-phenylnaphthylamine (PNA) as a background fluorophore for indirect fluorimetric detection in micellar electrokinetic capillary chromatography. The negative signal was primarily the result of quenching of the PNA by the analyte in the micelle's core. Reijenga et al. 10 presented an apparatus for simultaneous fluorescence and UV absorption detection in isotachophoresis. They demonstrated fluorescence quenching as a method of identification for non-fluorescent compounds, using quinine as a fluorescent counter ion. Stalikas et al. 11 reported an ion chromatographic method for the simultaneous determination of nitrite and nitrate by postcolumn indirect fluorescence detection with tryptophan as the fluorescent agent.Conductimetric and indirect UV absorption detection have been commonly utilized in ion chromatography. However, these detection methods are relatively universal and sometimes encounter a difficulty in identification of analytes, especially for complex mixtures. Selective detection is therefore preferred in the determination of ions of interest in a complex matrix. On the other hand, microcolumn ion chromatography has attracted a great deal of attention because it provides advantages over conventional ion chromatography in terms of mass sensitivity and decreased consumption of eluents. 12 Conductivity detectors for microcolumn ion chromatography are not commercially available, and direct UV and indirect UV absorption detection have been therefore utilized in microcolumn ion chromatography.Fluorimetric detection has been widely utilized in liquid chromatography because it provides sensitive and selective detection. Since most inorganic ions are not fluorescent, indirect fluorimetric detection is a good way for the determination of inorganic ions when fluorimetric detectors are used. However, there are few fluorescent ions available for the ion chromatography eluent, involving cerium(III) and salicylate. Indirect fluorimetric detection based on quenching is another option for the determination of ions in ion chromatography since there are a number of candidates. Indirect fluorimetric detection based on quenching is promising i...

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