Behavioural responses of the parasitoid Aphytis melinus to volatiles organic compounds (VOCs) from Aonidiella aurantii on its host fruit Tahitian lime fruit Citrus latifolia

Mohammed, Khalid; Agarwal, Manjree; Du, Xiaobo; Newman, J.; Ren, Yonglin

doi:10.1016/j.biocontrol.2019.03.015

Cited by 16 publications

(14 citation statements)

References 26 publications

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“…Among 34 VOCs extracted and identified by SPME and GC-MS techniques, only two VOCs, d-limonene, and β-ocimene were increased in all infested three citrus species, which were already known as a constituent of the odours of citrus fruit and citrus oils [16,20,23]. However, since d-limonene and β-ocimene are attractive to many other parasitoid species [19,20,24], these two compounds can be considered as putative VOCs as attractants of A. melinus. PCA analysis has highlighted that VOC emissions are particular for each species and chemical.…”

Section: Discussionmentioning

confidence: 99%

“…Since the variance in VOCs depends mainly on the fruit species, we cannot exclude the possibility that other VOCs produced specifically by one species or increased in all species may act as an attractant toward A. melinus. Indeed, non-infested fruit also produce VOCs that are attractive to parasitoids [25], and aphelinid parasitoids, such as A. melinus could be able to perceive cues from non-infested plants to locate a host [19,20]. Therefore, short-range VOCs produced by the plants or as feeding larvae secretion could be useful for successful localisation [27].…”

Section: Discussionmentioning

confidence: 99%

“…The VOCs d-limonene and β-ocimene have been reported as bouquet constituents of several citrus species [16,20]. d-Limonene and β-ocimene were identified as constituents of different infested fruit species that attracted other parasitoids such as Agathis bishopi and Aphidius gifuensis [24,32].…”

Section: Discussionmentioning

confidence: 99%

“…A. melinus females rely on stimuli to successfully locate their host. Aphytis use learned volatile cues from host plants as long-range attractants to potential habitats of their hosts [19,20]. Previous work with A. melinus and California red scale showed that wasps were attracted to the odours of scale-infested lemon fruit [21].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of d-Limonene and β-Ocimene as Attractants of Aphytis melinus (Hymenoptera: Aphelinidae), a Parasitoid of Aonidiella aurantii (Hemiptera: Diaspididae) on Citrus spp.

Mohammed

Agarwal

et al. 2020

Insects

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The volatile organic compounds (VOCs) released from herbivore-infested plants can be used as chemical signals by parasitoids during host location. In this research, we investigated the VOC chemical signals for the parasitoid Aphytis melinus to discriminate between Aonidiella aurantii (California red scale)-infested fruit and non-infested fruit on three different citrus species. First, we identified the chemical stimuli emanating from non-infested and A. aurantii-infested citrus fruits via solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) analyses and identified 34 volatile organic compounds (VOCs). The GC-MS analysis showed qualitative and quantitative differences between VOCs emitted from non-infested and infested citrus fruit. Two VOCs, d-limonene and β-ocimene, were significantly increased in all infested fruit, regardless of the fruit species. The response of the female adult A. melinus to olfactory cues associated with A. aurantii infested fruit was evaluated using a Y-tube olfactometer. In two-choice behavioural assays, A. melinus females preferred infested citrus cues over non-infested fruit. Females showed positive chemotaxis toward these VOCs in all tested combinations involving two dosages of synthetic compounds, d-limonene and β-ocimene, except for d-limonene at a dosage of 10 μL/mL. The application of these VOCs may help to enhance the effectiveness of bio-control programs and parasitoid mass-rearing techniques.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of d-Limonene and β-Ocimene as Attractants of Aphytis melinus (Hymenoptera: Aphelinidae), a Parasitoid of Aonidiella aurantii (Hemiptera: Diaspididae) on Citrus spp.

Mohammed

Agarwal

et al. 2020

Insects

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“…d ‐Limonene and β‐ocimene were significantly increased in fruit infested with Aonidiella aurantii and elicited a strong attraction for Aphytis melinus parasitoids 59 . Applications of these semiochemicals in the field may greatly enhance the efficiency of biological control of A. aurantia 60 . We also discovered that volatiles from honeydew produced by P. solenopsis were attractive to A. bambawalei , therefore treatments with more honeydew on plants triggered higher parasitism by A. bambawalei 61 .…”

Section: Discussionmentioning

confidence: 81%

Discovery of behaviorally active semiochemicals in Aenasius bambawalei using a reverse chemical ecology approach

Yang

Duan

et al. 2021

Pest Management Science

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BACKGROUND The invasive mealybug, Phenacoccus solenopsis, has caused serious damage to cotton crops throughout the world. Aenasius bambawalei is a dominant endoparasitoid of P. solenopsis. Exploration of behaviorally active semiochemicals may promote the efficacy of parasitoids used in biological control. Reverse chemical ecology, based on the physiological function of odorant‐binding proteins (OBPs), provides an effective approach to screen behaviorally active compounds to target insect pests. Determination of the binding mechanisms and specificity towards different odorants in A. bambawalei may facilitate the development of more‐efficient biological control strategies. RESULTS We characterized the expression profile and analyzed the binding affinity of OBP28 in A. bambawalei. AbamOBP28 showed high expression in the wings and antennae of both male and female A. bambawalei. A fluorescence competitive binding assay indicated that AbamOBP28 displayed strong binding affinity to most candidate ligands. Circular dichroism spectra demonstrated that 1‐octen‐3‐one, myrcene, dodecane, 2,4,4‐trimethyl‐2‐pentene, nonanal, and limonene elicited conformational changes in AbamOBP28. Electrophysiological and behavioral bioassays revealed that diethyl sebacate, 2,4,4‐trimethyl‐2‐pentene, and 1‐octen‐3‐one evoked significant electroantennography responses and functioned as attractants in A. bambawalei at specific concentrations. Furthermore, three‐dimensional structure modeling and molecular docking showed that hydrogen bonds were formed by Glu1 and Ser75 of AbamOBP28 with diethyl sebacate, respectively. CONCLUSION These results demonstrate that AbamOBP28 is involved in the chemoreception of A. bambawalei. The identified protein provides a potential target for efficient enemy utilization and pest control, and the overall results may help develop protocols for more effective screening of behaviorally active semiochemicals. © 2021 Society of Chemical Industry

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A parasitoid’s dilemma between food and host resources: the role of volatiles from nectar-providing marigolds and host-infested plants attracting Aphidius platensis

Souza

Silva

Silveira

et al. 2021

Sci Nat

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The use of nectar-producing companion plants in crops is a well-known strategy of conserving natural enemies in biological control. However, the role of floral volatiles in attracting parasitoids and effects on host location via herbivore-induced plant volatiles is poorly known. Here, we examined the role of floral volatiles from marigold (Tagetes erecta), alone or in combination with volatiles from sweet pepper plant (Capsicum annuum), in recruiting Aphidius platensis, an important parasitoid of the green peach aphid Myzus persicae. We also investigated whether marigold floral volatiles are more attractive to the parasitoid than those emitted by sweet pepper plants infested by M. persicae. Olfactometry assays indicated that floral volatiles attracted A. platensis to the marigold plant and are more attractive than sweet pepper plant volatiles. However, volatiles emitted by aphid-infested sweet pepper were as attractive to the parasitoid as those of uninfested or aphid-infested blooming marigold. The composition of volatile blends released by uninfested and aphid-infested plants differed between both blooming marigold and sweet pepper, but the parasitoid did not discriminate aphid-infested from uninfested blooming marigold. Volatile released from blooming marigold and sweet pepper shared several compounds, but that of blooming marigold contained larger amounts of fatty-acid derivatives and a different composition of terpenes. We discuss the potential implications of the aphid parasitoid attraction in a diversified crop management strategy.

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Behavioural responses of the parasitoid Aphytis melinus to volatiles organic compounds (VOCs) from Aonidiella aurantii on its host fruit Tahitian lime fruit Citrus latifolia

Cited by 16 publications

References 26 publications

Evaluation of d-Limonene and β-Ocimene as Attractants of Aphytis melinus (Hymenoptera: Aphelinidae), a Parasitoid of Aonidiella aurantii (Hemiptera: Diaspididae) on Citrus spp.

Evaluation of d-Limonene and β-Ocimene as Attractants of Aphytis melinus (Hymenoptera: Aphelinidae), a Parasitoid of Aonidiella aurantii (Hemiptera: Diaspididae) on Citrus spp.

Discovery of behaviorally active semiochemicals in Aenasius bambawalei using a reverse chemical ecology approach

A parasitoid’s dilemma between food and host resources: the role of volatiles from nectar-providing marigolds and host-infested plants attracting Aphidius platensis

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