Behavioural and sensory responses to some neem compounds by Pieris brassicae larvae

Luo, Lin’er; Loon, J.J.A. van; Schoonhoven, L. M.

doi:10.1111/j.1365-3032.1995.tb00809.x

Cited by 35 publications

(7 citation statements)

References 20 publications

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“…For example, azadirachtin exhibited a significant antifeedant effect on Drosophila melanogaster, Plutella xylostella , and Galleria mellonella (Sezer and Ozalp, 2011 ; Huang et al, 2016 ; Kilani-Morakchi et al, 2017 ). The antifeedant effect was mediated via the activation of deterrent receptor situated in the medial sensillum styloconicum (Liner et al, 1995 ). It could also inhibit the transmission of cholinergic nerve signaling and calcium channel in suboesophageal ganglion and reduce the frequency of miniature excitatory postsynaptic currents (mEPSCs).…”

Section: Introductionmentioning

confidence: 99%

Azadirachtin Affects the Growth of Spodoptera litura Fabricius by Inducing Apoptosis in Larval Midgut

et al. 2018

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Azadirachtin, the environmentally friendly botanical pesticide, has been used as an antifeedant and pest growth regulator in integrated pest management for decades. It has shown strong biological activity against Spodoptera litura, but the mechanism of toxicity remains unclear. The present study showed that azadirachtin inhibited the growth of S. litura larvae, which was resulted by structure destroy and size inhibition of the midgut. Digital gene expression (DGE) analysis of midgut suggested that azadirachtin regulated the transcriptional level of multiple unigenes involved in mitogen-activated protein kinase (MAPK) and calcium apoptotic signaling pathways. Simultaneously, the expression patterns of some differentially expressed unigenes were verified by quantitative real time-PCR (qRT-PCR). In addition, the enhanced terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) staining, the increased expression of caspase family members and apoptosis-binding motif 1 (IBM1) on both gene and protein level and the release of cytochrome c from mitochondria to cytoplasm were induced in midgut after azadirachtin treatment. These results demonstrated that azadirachtin induced structural alteration in S. litura larval midgut by apoptosis activation. These alterations may affect the digestion and absorption of nutrients and eventually lead to the growth inhibition of larvae.

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Section: Introductionmentioning

confidence: 99%

Azadirachtin Affects the Growth of Spodoptera litura Fabricius by Inducing Apoptosis in Larval Midgut

et al. 2018

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“…Among various options, neem has been identified as a source of environmentally "soft" natural pesticides. A number of compounds in neem kernel extract have been shown to have bioactivity equal or greater to that of Azadirachtin A (Lin-er et al 1995). Lower temperatures have been found to actually increase the efficacy of neem seeds 10-fold (Kabaru and Mwangi, 2000).…”

Section: Discussionmentioning

confidence: 99%

Effect of storage conditions and duration on the potency of Neem (Azadirachta indica A. Juss) seeds as a home-made Insecticide

El-Shafie¹,

Almahy²

2012

ABJNA

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This work was carried out to investigate the effects of grinding, storage conditions and duration on the bio-efficacy of neem seeds as a home-made insecticide against the red flour beetle Tribolium castaneum. Samples of neem seeds (intact and ground) were kept in small jute sacks and stored in the sun, refrigerator (4ºC), and room temperature (28 ± 2ºC) for two, four and six weeks in each condition. After the respective storage period, a water extract (5%) was prepared and sprayed topically on the 3 rd instar larvae of the beetle as well as on the pupal stage. A significant mortality of 62.83% resulted from the neem seeds stored in the refrigerator on the 7 th day of exposure. The seeds stored in the sun caused a mortality of 48.49%. The water extract from the seeds which were stored at room temperature, resulted in significantly high cumulative larval mortality of 50.30%. The efficacy of neem seeds kept in the sun was negatively affected and correlated with the length of storage period. However, the efficacy of the neem seeds stored in the refrigerator was increased with storage time. The pupal mortality due to spraying with water extract from neem seeds stored in the sun, refrigerator and room temperature was 49.67, 68.12 and 69.43% respectively. The mortality in the control (water treatment) was 7.33%. The results indicated that grinding of neem seeds before storage seems to have no effects on their efficacy.

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“…Extracts from different parts of the plantsbut mostly fruits of M. azedarachelicit a variety of effects in insects, such as antifeedant effects, growth inhibition, reduced fecundity, moulting disorders, morphogenetic defects, and changes of behavior [14,23,[25][26][27][28][29][30]. These effects have been attributed to the limonoids, which represent a large group of natural products found mainly in plants belonging to the Rutaceae and Meliaceae families [7,14,22].…”

Section: Identification Of Meliatoxins In Melia Azedarach Extracts Usmentioning

confidence: 99%

Identification of Meliatoxins in Melia azedarach Extracts Using Mass Spectrometry for Quality Control

et al. 2016

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Indiscriminate use of synthetic pesticides can be hazardous to both humans and the environment, but the use of natural products as a source of bio-based products, such as extracts, is an interesting approach to overcome these hazards. Unfortunately, the limonoids found in with desired insecticidal properties (e.g. azadirachtin) may also be present with limonoids toxic to mammals. The goal of this report was to develop a fast and reliable MS-based experiment to characterize meliatoxins in crude extracts of in order to provide unequivocal assessment of the safety for extracts for application in the field. MS and MS/MS experiments using MALDI ionization were evaluated as tools for the assignment of characteristic ions produced by each meliatoxin in crude extracts.The use of different experiments in combination, such as the analysis of fragment 557 and [M + Na] (adducts ions 681 andz 667), MALDI-MS can be used for detection of meliatoxins A/B or A/B in a crude extract and may be used to discriminate meliatoxins A from B, respectively. Subsequent MS/MS experiments can distinguish between the presence of group 1 and/or 2 in each class of meliatoxins classifying the proposed approach as a quick and efficient quality control method of meliatoxins in real samples.

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Behavioural and sensory responses to some neem compounds by Pieris brassicae larvae

Cited by 35 publications

References 20 publications

Azadirachtin Affects the Growth of Spodoptera litura Fabricius by Inducing Apoptosis in Larval Midgut

Azadirachtin Affects the Growth of Spodoptera litura Fabricius by Inducing Apoptosis in Larval Midgut

Effect of storage conditions and duration on the potency of Neem (Azadirachta indica A. Juss) seeds as a home-made Insecticide

Identification of Meliatoxins in Melia azedarach Extracts Using Mass Spectrometry for Quality Control

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