Behavioural and physiological responses to a conditioning protocol for adult zebrafish, Danio rerio, held in groups

Σαμαράς, Αθανάσιος; Pavlidis, M.

doi:10.1016/j.beproc.2020.104201

Cited by 4 publications

(2 citation statements)

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“…There have been developments in designing learning paradigms that are more appropriate for high-throughput screens ( Stewart et al, 2015 ). For example, automated systems for imaging, tracking, and analyzing dozens of larvae simultaneously ( Figure 1B ) ( Ahmed et al, 2010 ; Wolman et al, 2011 ; Doyle et al, 2016 ; Randlett et al, 2019 ; Barreiros et al, 2021 ); paradigms that can be performed with multiple adult fish at the same time ( Kareklas et al, 2018 ; Samaras and Pavlidis, 2020 ; Barreiros et al, 2021 ); and relatively short paradigms that do not last for more than 2 or 3 days ( Hinz et al, 2013 ; Lucon-Xiccato and Dadda, 2014 ). Efforts should be made to ensure that fish are habituated properly to the paradigm setup and show no signs of stress or anxiety, especially for paradigms that use individual zebrafish ( Gerlai, 2016 ).…”

Section: Zebrafish As a Model For Studying Learning And Memorymentioning

confidence: 99%

Learning and memory formation in zebrafish: Protein dynamics and molecular tools

Reemst

Shahin²,

Shahar³

2023

Front. Cell Dev. Biol.

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Research on learning and memory formation at the level of neural networks, as well as at the molecular level, is challenging due to the immense complexity of the brain. The zebrafish as a genetically tractable model organism can overcome many of the current challenges of studying molecular mechanisms of learning and memory formation. Zebrafish have a translucent, smaller and more accessible brain than that of mammals, allowing imaging of the entire brain during behavioral manipulations. Recent years have seen an extensive increase in published brain research describing the use of zebrafish for the study of learning and memory. Nevertheless, due to the complexity of the brain comprising many neural cell types that are difficult to isolate, it has been difficult to elucidate neural networks and molecular mechanisms involved in memory formation in an unbiased manner, even in zebrafish larvae. Therefore, data regarding the identity, location, and intensity of nascent proteins during memory formation is still sparse and our understanding of the molecular networks remains limited, indicating a need for new techniques. Here, we review recent progress in establishing learning paradigms for zebrafish and the development of methods to elucidate neural and molecular networks of learning. We describe various types of learning and highlight directions for future studies, focusing on molecular mechanisms of long-term memory formation and promising state-of-the-art techniques such as cell-type-specific metabolic labeling.

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Section: Zebrafish As a Model For Studying Learning And Memorymentioning

confidence: 99%

Learning and memory formation in zebrafish: Protein dynamics and molecular tools

Reemst

Shahin²,

Shahar³

2023

Front. Cell Dev. Biol.

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“…They are used as functional markers of activity in neurons and neuronal circuitries after a variety of stimuli/stressors (e.g., [135][136][137][138][139]). C-fos can be used as a potent indicator of the neuronal activity of memory retrieval after conditioning to aversive stimuli, and a marker of the stress response [140], including in the aquaculture environment [141,142]. Early-life stress and acute restrain stress were found to change the expression of c-fos in brain areas of seabream (Sparus aurata) [84] and zebrafish [143], respectively.…”

Section: The Neuroepigenetics Of Stress: Fishing With Mammalian Modelsmentioning

confidence: 99%

Epigenetics of Stress in Farmed Fish

Guinand

Σαμαράς

2023

Epigenetics in Aquaculture

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As the rearing environment and hatchery practices are stressful, there is an increased interest on how epigenetics could impact or monitor health and welfare of aquaculture species, and further participate to improve production. However, while epigenetic modifications inducing neurobehavioral changes after exposure to acute or chronic stress have been shown to be integral parts of the observed stress response in mammals, the present-day literature on the socalled 'epigenetics of stress' in fish is scarce. As factors that cause stress affect the central nervous system are mediated to the whole body by the hypothalamic-pituitary-adrenal (interrenal) (HPA/HPI) system, we argue that concentrating on the brain is of prime importance to progress in the epigenetics of stress in aquaculture. This knowledge should promote new opportunities for perhaps more integrative and comparative epigenetic monitoring of the impact of early-life stress in cultured fish species.

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