Behaviour of Some Enzymes and Isoenzymes in Plasma
Liver and Bile of Rats Treated with Carbon Tetrachloride

Idéo, G.; E, Del Ninno; R, de Franchis

doi:10.1159/000459537

Cited by 15 publications

(3 citation statements)

References 6 publications

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“…A control group was given mineral oil only. In previous experiments this dose proved to be effective in producing liver cell necrosis within 48 h [8],…”

Section: Liver Necrosis Groupmentioning

confidence: 81%

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

1972

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γ-GT serum levels have been compared in various liver diseases with AP and LAP, cholestatic markers ; with GOT and GPT, the increases of which are related to cellular injury. γ-GT levels have also been assayed in serum and in hepatic tissue of rats after poisoning with CCl₄ and after bile duct ligation. The results showed in all cases a significant correlation between γ-GT and AP, and γ-GT and LAP. A significant increase of γ-GT was found in plasma and in hepatic tissue of rats after bile duct ligation; on the other hand normal levels were found in the plasma of rats after treatment with CCl₄. γ-GT gelfiltration on Sephadex G-200 showed one peak only of activity in all cases considered, eluted with the first protein fraction. The enzymes seem to be rather homogeneous with a molecular weight of about 200,000.

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“…A control group was given mineral oil only. In previous experiments this dose proved to be effective in producing liver cell necrosis within 48 h [8],…”

Section: Liver Necrosis Groupmentioning

confidence: 81%

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

1972

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“…Thus, whereas Koide and Oda [13], and Ideo et al [18] reported an increase in this enzyme at between 6 and 24 h in rats treated with CCI4, [15], and Zimmer man et al [16,17] found no variations in the activity of this enzyme. As far as we are con cerned, we did not find any significant vari ations in the levels of G6Pase throughout the entire study either.…”

Section: Discussionmentioning

confidence: 91%

Serum Activity of the Key Gluconeogenic Enzymes in Carbon-Tetrachloride-Induced Experimental Hepatotoxicity

Morata¹,

Colmenero²,

Sanchez-Carrillo³

et al. 1990

Enzyme

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Serum activity has been measured in three of the key enzymes in the gluconeogenic pathway in rats subjected to experimental hepatotoxicity after intraperitoneal administration of carbon tetrachloride. The levels of phosphoenolpyruvate carboxykinase (PEPCK) and fructose-1,6-biphosphatase (FBPase) showed a similar behavior to the transaminase (AST and ALT), increasing markedly with respect to the controls at 12 h after administration of the poison, reaching their maximum peak of activity at between 24 and 36 h, and returning to normal values at 96 h. The activity of glucose-6-phosphatase was not significantly modified throughout the treatment. These results seem to demonstrate that the determination of the serum activity of PEPCK and FBPase could be a sensitive and specific marker of hepatic cytolysis.

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“…Homogenates were rapidly frozen, thawed and submitted to ultrasonic treatment (3 times 20 s), then centrifuged for 20 min at 10000 g. The supernatant fluid was employed for the enzyme assay. This method was shown to be suitable for enzymatic estimations in liver homogenates (10).…”

Section: Methodsmentioning

confidence: 99%

Biochemical and Histochemical Estimations of Liver γ-Glutamyltransferase Activity in Ethanol-Fed Rats

Idéo¹,

Tempini²,

Bellobuono³

et al. 1980

CCLM

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Ethanol-fed rats showed significantly elevated plasma and liver 7-glutamyltransferase activity, compared with rats fed carbohydrates isocalorically, or with untreated control animals. The activity of-glutamyltransferase in liver was detected by means of both biochemical and histochemical methods. These data suggest that increased plasma-glutamyltransferase activity commonly found in alcoholics can be related, at least in part, to a hepatic enhancement of the activity in the liver. Biochemische und histochemische Untersuchung der Aktivität von y-Glutamyltransferase in der Leber JEthanolgefütterter Ratten Zusammenfassung: Ethanol-gefutterte Ratten zeigten signifikant erhöhte katalytische Aktivitäten von 7-Glutamyltransferase in Plasma und Leber, verglichen mit isokalorisch Kohlenhydrat-ernährten oder unbehandelten Kontrolltieren. Die Aktivität der-Glutamyltransferase in der Leber wurde mit bio-und histochemischen Methoden nachgewiesen. Die Ergebnisse weisen darauf hin, daß die gewöhnlich im Plasma von Alkoholikern gefundene Erhöhung der Aktivität von-Glutamyltransferase mindestens teilweise auf deren Zunahme in der Leber bezogen werden kann.

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Behaviour of Some Enzymes and Isoenzymes in Plasma Liver and Bile of Rats Treated with Carbon Tetrachloride

Cited by 15 publications

References 6 publications

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

γ-Glutamyl Transpeptidase: a Clinical and Experimental Study

Serum Activity of the Key Gluconeogenic Enzymes in Carbon-Tetrachloride-Induced Experimental Hepatotoxicity

Biochemical and Histochemical Estimations of Liver γ-Glutamyltransferase Activity in Ethanol-Fed Rats

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