Behaviour of ring bivalents in holokinetic systems: Alternative sites of spindle attachment in Pachylis argentinus and Nezara viridula (Heteroptera)

Papeschi, Alba Graciela; Mola, Liliana M.; Bressa, María José; Greizerstein, Eduardo José; Lia, Verónica Viviana; Poggio, Lidia

doi:10.1023/b:chro.0000005740.56221.03

Cited by 50 publications

(60 citation statements)

References 22 publications

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“…Two m-chromosomes remain separated till diplotene, get associated subsequently to form a negatively heteropycnotic pseudobivalent that lacks chiasma and usually occupies centre of the plate as is also observed in other coreids (Sands 1982a, b andPapeschi et al 2003). At metaphase-I, a definite pattern of arrangement is observed in both the species.In Serinetha augur, the autosomes and single bipartite X are arranged in a circle, while m-pseudobivalent lies in the centre as is also observed in Ochrochira rubrotinita and Pachylis argentinus (Sands 1982a, b, Papeschi et al 2003.…”

Section: Coreidaesupporting

confidence: 68%

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Chromosomes and their Meiotic Behavior in Two Families of the Sub-order Heteroptera

et al. 2006

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Summary Eurydema pulchrum Westw. and Bagrada cruciferarum Kirkadly (Pentatomidae) show 2nϭ14ϭ12AϩXY. Autosomes show gradation in size, X is of medium size while Y is the smallest element. Both X and Y are positively heteropycnotic in diffused stage but become isopycnotic in diplotene stage and divide equationally during meiosis-I. At metaphase-II, X and Y get associated, the association being strong forming a pseudobivalent XY in Eurydema pulchrum while weak in Bagrada cruciferarum. A definite pattern of arrangement of the chromosomes is seen at metaphase-II.Cletus bipunctatus Westw. and Serinetha augur Fabr. (Coreidae) show 2nϭ17ϭ14Aϩ2mϩXO and 2nϭ13ϭ10Aϩ2mϩXO respectively. The complement in both the species is conspicuous by the presence of 2 microchromosomes and absence of Y element in males. Microchromosomes form achiasmatic bivalent at meiosis-I. X undergoes equational division at metaphase-I while at metaphase-II it remains outside the metaphase plate and is added to one of the pole as a precocious element in Cletus bipunctatus and as a laggard in Serinetha augur.Key words Chromosomes, Meiosis, Pentatomidae, Coreidae.The family Pentatomidae, one of the major families of Heteroptera, includes shield-shaped bugs which are commonly called stink-bugs due to the presence of stink glands. Members of the family Coreidae are called squash bugs or leaf-footed bugs and are characterized by the presence of scent glands on the thorax. Family Pentatomidae is noted for its extreme karyotypic conservation. Earlier knowledge about the cytology of this family is largely due to the contribution made by Gupta (1950), Manna (1951, Rao (1954), Dutt (1955, Hughes-Schrader and Schrader (1957), Parshad (1957b) and Schrader and Hughes-Schrader (1958). The family Coreidae is characterized by the presence of a pair of very small chromosomes which behave differently from both autosomes and sex-chromosomes during meiosis (Wilson 1905). The present paper includes the study of diploid complement and behavior of autosomes and sex chromosomes during meiotic cycle in four North Indian heteropteran species belonging to Pentatomidae and Coreidae. Materials and methodsSpecimens of Eurydema pulchrum Westw., Bagrada cruciferarum Kirkadly. (Pentatomidae) and Serinetha augur Fabr. (Coreidae) were collected from Patiala (Punjab) while those of Cletus bipunctatus Westw. (Coreidae) were collected from Palampur (Himachal Pradesh). Male bugs of each species were dissected to take out the testes which were fixed in ethanol : glacial acetic acid (3 : 1) and air dried chromosomal preparations were stained in carbolfuchsin stain.

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Section: Coreidaesupporting

confidence: 68%

“…Then after, Y becomes the lightest element of the complement as is also recorded in Coridius janus, Andrallus spinidens and Nezara viridula by Satapathy and Patnaik (1991). Papeschi et al (2003), however, observed both X and Y to be positively heteropycnotic till diakinesis in Nezara viridula.…”

Section: Discussionmentioning

confidence: 68%

Chromosomes and their Meiotic Behavior in Two Families of the Sub-order Heteroptera

et al. 2006

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“…In some Heteroptera, a single NOR was observed, which could be interstitial or terminal and located at autosomes or sex chromosomes (Camacho et al, 1985;Fossey and Liebenberg, 1995;González-García et al, 1996;Papeschi et al, 2003;Rebagliati et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Morphological patterns of the heteropycnotic chromatin and nucleolar material in meiosis and spermiogenesis of some Pentatomidae (Heteroptera)

et al. 2008

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Pentatomidae is a family of Heteroptera which includes several agriculture pests that have had different aspects of their meiosis and spermiogenesis analyzed. In the present study we analyzed the morphological patterns of the heteropycnotic chromatin and the nucleolar material of Mormidea v-luteum, Oebalus poecilus and Oebalus ypsilongriseus. The three species presented multilobate testes, with three lobes in M. v-luteum and four in the Oebalus species. A karyotype with 2n = 14 chromosomes (12A + XY) was observed in the three species. Several characteristics were common to the three species, such as the absence of a testicular harlequin lobe (a lobe which produces different types of spermatozoa, previously considered a general characteristic of this family), late migration of the sex chromosomes and semi-persistence of the nucleolus. The three species also shared some characteristics regarding the patterns of the heteropycnotic chromatin and nucleolar material, but differed in others mainly related to the location of the heteropycnotic chromatin in the spermatids and the morphology and distribution of the nucleolar material at zygotene. The differences were always between species from different genera, suggesting a relationship with their genetic divergence.

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“…In many cases, only 1 GC-rich heterochromatic block (interstitial or terminal) is recognized, which is often associated with the nucleolus organizer region (NOR; Camacho et al, 1985;Papeschi et al, 2003;Grozeva et al, 2004). Reports of interstitial heterochromatic blocks scattered throughout most autosomes are scarce and restricted to the family Curiae (Holhymenia rubiginosa and Spartocera batatas) (Fabricius, 1798) (Franco et al, 2006;Bressa et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…These studies revealed the distribution of 18S rDNA sites in autosomal chromosomes, such as those observed in Pachylis argentinus Berg, 1879, and Nezara viridula Linnaeus, 1758 (Papeschi et al, 2003), as well as in sex chromosomes such as those of the families Belostomatidae , Cimicidae (Grozeva et al, 2010), and Reduviidae (Bardella et al, 2010). Although studies investigating the location of heterochromatin are more common in Heteroptera, most of them are restricted to conventional analyses such as C-banding technique .…”

Section: Introductionmentioning

confidence: 99%

Physical mapping of 18S rDNA and heterochromatin in species of family Lygaeidae (Hemiptera: Heteroptera)

Bardella¹,

Sampaio²,

Venturelli³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Analyses conducted using repetitive DNAs have contributed to better understanding the chromosome structure and evolution of several species of insects. There are few data on the organization, localization, and evolutionary behavior of repetitive DNA in the family Lygaeidae, especially in Brazilian species. To elucidate the physical mapping and evolutionary events that involve these sequences, we cytogenetically analyzed three species of Lygaeidae and found 2n (♂) = 18 (16 + XY) for Oncopeltus femoralis; 2n (♂) = 14 (12 + XY) for Ochrimnus sagax; and 2n (♂) = 12 (10 + XY) for Lygaeus peruvianus. Each species showed different quantities of heterochromatin, which also showed variation in their molecular composition by fluorochrome Physical mapping in Lygaeidae staining. Amplification of the 18S rDNA generated a fragment of approximately 787 bp. The alignment of the consensus sequence with sequences from other species of Heteroptera deposited in the GenBank revealed a similarity of 98% with small differences. Fluorescent in situ hybridization with the 18S rDNA fragment revealed that this ribosomal gene was located in 1 autosomal pair at different positions in the three species. No cytogenetic data are available for these Brazilian species. The basal number and the possible chromosomal changes that occurred among the different species, as well as the evolution of these DNA sequences, are discussed.

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Behaviour of ring bivalents in holokinetic systems: Alternative sites of spindle attachment in Pachylis argentinus and Nezara viridula (Heteroptera)

Cited by 50 publications

References 22 publications

Chromosomes and their Meiotic Behavior in Two Families of the Sub-order Heteroptera

Chromosomes and their Meiotic Behavior in Two Families of the Sub-order Heteroptera

Morphological patterns of the heteropycnotic chromatin and nucleolar material in meiosis and spermiogenesis of some Pentatomidae (Heteroptera)

Physical mapping of 18S rDNA and heterochromatin in species of family Lygaeidae (Hemiptera: Heteroptera)

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