Soil samples were collected from four aquaculture ponds (yellow perch culture), a control pond (without aquaculture activities, fallow pond), crop land (under corn), and forest land to estimate the carbon (C) sequestration potential in the Piketon county, Ohio, USA. The averaged total of C was 6.5 ± 2, 8.8 ± 2, 8.53 ± 0.2 and 10.49 ± 1.1 Mg/ha (Mg=10(6)g) in < 0.25 mm fraction; 15.2 ± 2, 16.0 ± 3, 11.49 ± 0.8 and 17.23 ± 3.4 Mg/ha in micro aggregates (0.25-2.5 mm); and 22.1 ± 3, 26.4 ± 3, 12.16 ± 1.6 and 18.51 ± 4.3 Mg/ha in macro aggregates ( > 2.5mm), for aquaculture ponds, control ponds, cropland and forest land, respectively. The soil/sediment C pool followed the order of forest > crop land soils > aquaculture pond soils.