Behaviour of <i>Bacillus stearothermophilus</i> Grown in Different Media

Atkinson, A.; Evans, Celia G.; Yeo, R. G.

doi:10.1111/j.1365-2672.1975.tb00533.x

Cited by 17 publications

(5 citation statements)

References 6 publications

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“…However the growth yield (0.53 mg dry wt · mL -1 ) was low compared with those of other heterotrophic thermophilic bacilli (Atkinson et al 1975;Khraptosova and Loginova 1981). This fact could be explained by the conditions prevailing in standard cultures.…”

Section: Discussionmentioning

confidence: 96%

Heterotrophic nitrification by a thermophilicBacillusspecies as influenced by different culture conditions

Mével¹,

Prieur²

2000

Can. J. Microbiol.

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The nitrification activity of a thermophilic heterotrophic bacterium, Bacillus MS30 isolated from a deep-sea hydrothermal vent, was studied under various growth conditions. Nitrification was estimated from the nitrogen balance calculations in the culture media. The results showed that this isolate actively nitrified in culture conditions similar to those prevailing in hydrothermal sites. Therefore, its ecological significance was considered. In standard aerobic conditions, MS30 produced nitrite from ammonia and acetate (1.13 mumol NO2-.mg-1 dry wt), but nitrate was never produced, and a low nitrite reduction was often observed. Higher nitrification activities were observed in defined optimal conditions (simple carbon substrate, 65 degrees C, pH 7.5, and 15 g sea salts.L-1). In addition, discrepancies between the optima for growth and nitrification were observed, showing the ability of MS30 to adapt to changing environmental conditions typical of hydrothermal sites.

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Section: Discussionmentioning

confidence: 96%

Heterotrophic nitrification by a thermophilicBacillusspecies as influenced by different culture conditions

Mével¹,

Prieur²

2000

Can. J. Microbiol.

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“…These included : increase and decrease of medium components, change of base to NH,OH to check possible inhibition by K+ as reported by Atkinson et al (1975), and different protocols for initiating continuous culture (e.g. exponential growth vs stationary phase).…”

Section: Continuous Cultures On 15 and 20 G Sucrose I-'mentioning

confidence: 99%

Pathways of ethanol production from sucrose by a mutant thermophilic Bacillus in continuous culture

Martín

Bushell

Leak³

et al. 1993

Journal of General Microbiology

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A mutant (strain LLD-15) of Bacillus stearothevmuphihs strain LLD-R, that lacks L-lactate dehydrogenase activity was studied in anaerobic single-stage continuous culture using a defined minimal medium for its capacity to produce ethanol from sucrose at 70 "C. Cultures were stable at neutral pH, dilution rates below 0.25 h-' and sucrose concentrations below 15 g 1-' , producing mainly ethanol, CO,, formate and acetate plus a little succinate. The data obtained were used to estimate the maintenance energy coefficient at 70 "C and showed that the relative fluxes through the pyruvate-formate lyase (PFL) pathway and an anaerobic pyruvate dehydrogenase (PDH) pathway were about equal, though the later predominates at low dilution rates. At higher dilution rates or at acid pH, L-lactate became the major anaerobic product due to takeover by wild-type revertants. Reversion occurred only above a critical specific sucrose consumption rate of 4.2 g sucrose h-' (g cells)-' at pH 7, at which point pyruvate production was also seen. This can be attributed to saturation of the PFL pathway and PDH pathways by increasing glycolytic flux and consequent accumulation of intracellular pyruvate. The growth rate then declines, but a small population of revertant LLD-R cells would always be present in the bioreactor since the mutation in the L-lactate dehydrogenase gene has a significant reversion rate. These revertants have no selective advantage below the critical sugar uptake rate, but at higher rates they divert excess pyruvate to L-lactate, so their growth is unimpaired and they take over in the culture. At lower pH, growth restriction and reversion occur at low sucrose consumption rates due to difficulty in excreting acetic and formic acids against a pH gradient.

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“…In the above connection, it should be mentioned that the reported glycerol kinase activity of B. stearothermophilus grown on glycerol in a rich medium, in batch culture, was relatively low [200 units (g dry cells)-l, or 0-4 ymol min-l (mg protein-')] and lower than that of cells grown on glycerol in a simple salts medium (Atkinson et al, 1975;Atkinson & Comer, 1983). The sensitivity to glycerol of glycerol-sufficient cells was not uniform but varied with the nature of the growth limitation.…”

Section: Enzymes Of Glycerol Metabolism In Glycerol-limited Cellsmentioning

confidence: 98%

Growth of Bacillus stearothermophilus on glycerol in chemostat culture: expression of an unusual phenotype

Burke

Tempest

1990

Journal of General Microbiology

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Bacillus stearothermophilus grew readily on glycerol in carbon-limited chemostat culture and expressed a high carbon conversion efficiency. However, the strain of organism used (probably B. stearothermophilus var. nondiastaticus) proved particularly sensitive to glycerol, both respiration and growth being severely impeded by any surfeit of this compound. Sensitivity was found to correlate with an exceptionally high level of expression of glycerol kinase [activities of more than 80 pmol min-' (mg protein)-' were manifest in crude cell-free extracts), coupled with low activities of methylglyoxal synthase and of glyoxylase (enzymes of the methylglyoxal bypass). It is proposed that metabolic dysfunction results from an uncontrolled gross accumulation of glycerol phosphate (and early products of its metabolism) within the cells, coupled with depletion of the intracellular phosphate pool.

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Behaviour of Bacillus stearothermophilus Grown in Different Media

Cited by 17 publications

References 6 publications

Heterotrophic nitrification by a thermophilicBacillusspecies as influenced by different culture conditions

Heterotrophic nitrification by a thermophilicBacillusspecies as influenced by different culture conditions

Pathways of ethanol production from sucrose by a mutant thermophilic Bacillus in continuous culture

Growth of Bacillus stearothermophilus on glycerol in chemostat culture: expression of an unusual phenotype

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