Behaviour of Dicentric Chromosomes in Peas

Saccardo, F.

doi:10.1080/00087114.1971.10796415

Cited by 8 publications

(2 citation statements)

References 35 publications

(14 reference statements)

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“…We also observed that plants heterozygous for LexA-CENH3(v2) and ABS4 tended to be short and have asymmetric leaves (Figure S5), suggesting that the loss and gain of genes on chromosome 4L negatively impacted plant development. Similar phenotypes were observed in pea plants carrying a dicentric chromosome (33).…”

Section: Chromosome Breakage In Somatic Tissues Of Abs4/lexa-cenh3 Linessupporting

confidence: 78%

“…The two breaks can then fuse (by non-homologous end joining) in the next cell cycle to recover the original dicentric condition, leading to repeated cycles of breakage and rejoining ( 30 ). Another outcome is that the breaks can heal by acquiring telomeres, separating the previously dicentric chromosome into two independent chromosomes ( 30, 33 ). In a minority of cases, the bridge is pulled to one pole without breaking ( 31, 34 ).…”

Section: Introductionmentioning

confidence: 99%

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Synthetic maize centromeres transmit chromosomes across generations

Dawe

Gent

Zeng

et al. 2022

Preprint

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Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering centromeres that function in vivo has proven to be difficult. Here we describe a LexA-CENH3 tethering approach that activates functional centromeres at maize synthetic repeat arrays containing LexO binding sites. The synthetic centromeres are sufficient to cause chromosome breakage and release of chromosome fragments that are passed through meiosis and into progeny. Several independent chromosomes were identified, each with newly created centromeres localized over the repeat arrays where they were directed. The new centromeres were self-sustaining and stably transmitted chromosomes to progeny in the absence of the LexA-CENH3 activator. Our results demonstrate the feasibility of using synthetic centromeres for karyotype engineering applications.

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Section: Chromosome Breakage In Somatic Tissues Of Abs4/lexa-cenh3 Linessupporting

confidence: 78%