Behavioral responses of different reproductive statuses and sexes in Hermetia illucens (L) adults to different attractants

Abstract

Background The odor of various fermented organic materials acts as an attractant for oviposition by gravid females of the black soldier fly (BSF) to find larval food sources. Females display oviposition site selection on various organic materials, but little work has been done on the response to substrate attractants under caged conditions similar to those in a BSF farm production system. Methods Fifty of each reproductive status (mated and virgin) and sex (males and females) of BSF adults were marked and then exposed to one of five different oviposition attractants in a transparent acrylic chamber: no substrate (control) plus pineapple, mixed vegetables, okara, and fermented fish to represent fruit-, vegetable-, plant protein-, and animal protein-based substrates, respectively. The frequency of the perching activity on the oviposition apparatus and flying behavior under the LED illumination, including the laid egg weight, were recorded. Results The sexually-related activities of BSF adults were clearly observed. A majority of the females preferred to perch on the oviposition apparatus and fly around the illuminated area compared to the very low activities of the mated males. The BSF adults displayed different behavioral responses to the different tested attractants. While active flying was common when using plant protein- and animal protein-based substrates, mated females showed the greatest perching preference for plant-based substrates (fruit and vegetables) and this correlated with the laid egg weight. Discussion Egg-laying was more likely to happen on the plant-based substrate than on the animal protein-based substrate. However, the strong smell of the animal protein-based substrate could strongly trigger lekking behavior, which is an important part of mating behavior. This knowledge can support egg trapping in nature and also improve the efficiency of egg production in mass-rearing facilities.

Use of different dry materials to control the moisture in a black soldier fly (Hermetia illucens) rearing substrate

“…The flies were provided with a 30% (w/v) brown sugar solution, which was sprayed onto the mesh chamber three times a day to serve as a food source. Oviposition devices, consisting of a tray filled with an attractant substrate made from a mixture of wheat bran, fruits, and vegetables ( Khaekratoke, Laksanawimol & Thancharoen, 2022 ; Laksanawimol, Singsa & Thancharoen, 2023 ), were placed within the chamber. Wooden sheet pieces were also provided as oviposition substrates placed above the tray of attractant materials.…”

Reproduction and Production Traits of Eggiest of Black Soldier Fly Reared in Different Enriched Media and its Potential as Poultry Feed

“…This can be seen from the increasing number of cells of red blood cells, white blood cells, and the number of cells that carry out phagocytic activity (Ottoboni et al, 2020). Adult maggots do not eat, but only need water because nutrients are only needed for reproduction during the larval phase (Laksanawimol et al, 2023). Hermetia illucens in its life cycle does not perch on food that is directly consumed by humans.…”