Behavioral Response of Lygus hesperus to Conspecifics and Headspace Volatiles of Alfalfa in a Y-Tube Olfactometer

Blackmer, Jacquelyn L.; Rodriguez‐Saona, Cesar; Byers, John A.; Shope, K. L.; Smith, J. P.

doi:10.1023/b:joec.0000042067.27698.30

Cited by 105 publications

(116 citation statements)

References 17 publications

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“…However, the present study also indicated that a single, relatively ubiquitous HIPV can be attractive to A. iole and can lead to increased parasitism. The green-leaf volatile, (Z)-3-hexenyl acetate; the sesquiterpene, α-farnesene; and the carboxylate ester, methyl salicylate, are all produced and released by plants in response to injury by Lygus species (Rodriguez-Saona et al 2002;Blackmer et al 2004;Williams et al 2005) and by other herbivores (Turlings et al 1991;Loughrin et al 1995;Röse et al 1996;Paré and Tumlinson 1998). Thus, it appears that A. iole can use either host-specific or general volatile cues in the process of host habitat location.…”

Section: Discussionmentioning

confidence: 99%

“…Naïve wasps responded to methyl salicylate and α-farnesene but needed to learn host-plant odors before responding to (Z)-3-hexenyl acetate. These three volatile compounds are common in many plant species, and one or more of them are induced in cotton, maize, and alfalfa following herbivory by L. hesperus (Rodriguez-Saona et al 2002;Blackmer et al 2004;Williams et al 2005). It is, therefore, not surprising that A. iole uses more than one volatile cue to find hosts; still, a single compound was sufficient to attract wasps in both laboratory and field experiments.…”

Section: Discussionmentioning

confidence: 99%

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EAG-Active Herbivore-Induced Plant Volatiles Modify Behavioral Responses and Host Attack by An Egg Parasitoid

et al. 2008

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Volatiles emitted by plants in response to feeding by Lygus species were tested in neurophysiological, behavioral, and parasitism trials with Anaphes iole, an egg parasitoid of Lygus. Electroantennogram analyses indicated that A. iole antennae responded to most herbivore-induced plant volatiles (HIPVs) tested and that females were usually more responsive than males. Antennal responses to (Z)-3-hexenyl acetate and methyl salicylate were among the strongest. Behavioral assays in a four-arm olfactometer demonstrated that response of female wasps to (Z)-3-hexenyl acetate varied greatly depending on preconditioning regime. Preconditioning wasps to complex host-plant odors led to stronger preference than did a single preconditioning stimulus, i.e., (Z)-3-hexenyl acetate. In a horizontal wind tunnel, female wasps were attracted by methyl salicylate and α-farnesene. Parasitism of Lygus lineolaris eggs by A. iole in a cotton field was greater when the eggs were associated with (Z)-3-hexenyl acetate or α-farnesene than with controls. Overall, the results of this study show that A. iole can perceive a variety of plant volatiles released after its host damages plants, that the degree of associative learning in A. iole can be manipulated based on preconditioning regime, and that single synthetic HIPVs are attractive to A. iole and can be used to increase attack rates on host eggs. Therefore, it appears that HIPVs have potential for use in suppression of Lygus population densities.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

EAG-Active Herbivore-Induced Plant Volatiles Modify Behavioral Responses and Host Attack by An Egg Parasitoid

et al. 2008

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“…Unless otherwise stated, L. hesperus (collected from Maricopa, AZ, USA) was reared in the laboratory on green beans and synthetic diet (Rodriguez-Saona et al, 2002;Blackmer et al, 2004). Sexes were separated by the presence or absence of an ovipositor groove.…”

Section: Induced Release Of Defensive Chemicals From Adult Tarnished mentioning

confidence: 99%

Production and Predator-Induced Release of Volatile Chemicals by the Plant Bug Lygus hesperus

Byers

2006

J Chem Ecol

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Both sexes of adult western tarnished plant bug, Lygus hesperus Knight (Heteroptera: Miridae), released three volatile chemicals in relatively large amounts when attacked by ants (Pogonomyrmex rugosus and Solenopsis xyloni) or when grabbed by forceps, as determined by solid-phase microextraction (SPME) and gas chromatographymass spectrometry (GC-MS). The relative amounts of the volatile compounds, hexyl butyrate, (E)-4-oxo-2-hexenal, and (E)-2-hexenyl butyrate, absorbed by SPME as a percentage of the largest were 100%, 44%, and 4%, respectively, from females, and 83%, 37%, and 3% from males. Both ant species were repelled by the defensive discharges (confirmed by SPME) when the ants attacked L. hesperus adults. Sexually mature L. hesperus were individually extracted in pentane to quantify the mean amounts of hexyl butyrate (14.9 μg/female; 10.3 μg/male), (E)-4-oxo-2-hexenal (2.7 μg/female; 3.1 μg/ male), and (E)-2-hexenyl butyrate (1.2 μg/female; 0.6 μg/male). (E)-4-Oxo-2-hexenal was unstable in solvent when in contact with a macerated adult, but relatively stable when the solution was decanted within minutes. The production of the three major volatile components began soon after the emergence of the adult and amounts increased for about 5-10 d with little or no increase thereafter. Minor additional constituents were crosscorrelated in many cases with the three major ones. A cost of defensive secretion is suggested for females but not for males, because heavier females produced more volatile compounds than lighter females. The initial discharge percentage, defined as the proportion of volatile compounds initially present that is discharged to defend against predation was

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“…Incoming air was filtered through activated charcoal and humidified with doubly distilled, deionized water. The filtered air was split between two, 2-1 holding chambers [2]; one chamber, holding the kairomone blend (1:1:1 ratio of (+)-α-pinene:(-)-β-pinene:(+)-3-carene), served as a positive control and the other chamber held the kairomone blend plus NHVs. The kairomone blend (100 µg) and NHVs (100 µg), in 10 µL of hexane, were applied to a filter paper strip (5 × 50 mm).…”

Section: Y-tube Olfactometer Trialsmentioning

confidence: 99%

Réponses électrophysiologiques et comportementales de Dendroctonus valens à des composés volatils non-hôtes

2007

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-Non-host volatiles (NHVs) that are often reported as being disruptive to coniferophagous bark beetles were tested for both electrophysiological and behavioral effects on the red turpentine beetle, Dendroctonus valens LeConte (Coleoptera: Curculionidae: Scolytinae), which was accidentally introduced into China in the mid-1980's. All NHVs tested elicited dose-dependent antennal responses by D. valens. In Y-tube olfactometer trials, D. valens were repelled by NHVs tested. When NHVs were added to a kairomone blend, responses of D. valens were significantly inhibited. Further field trapping experiments showed that attraction of D. valens to kairomone baited traps was reduced by all individual NHVs, with reductions ranging from 26.3 to 70%. 1-Octen-3-ol, (Z)-3-hexen-1-ol, and (E)-2-hexen-1-ol were the three most effective NHVs, significantly reducing D. valens to kairomonebaited traps by 69.5, 68.3 and 66.0%, respectively. In the development and implementation of a semiochemical-based management programme for D. valens, NHVs may have considerable potential for disrupting the beetle's ability to locate suitable hosts.non-host volatiles / red turpentine beetle / electroantennograms / olfactory response / field trapping Résumé -Réponses électrophysiologiques et comportementales de Dendroctonus valens à des composés volatils non-hôtes. Au cours de cette étude, on a testé les effets de composés volatils non hôtes, souvent considérés dans la littérature comme pouvant affecter la reconnaissance de l'hôte chez les scolytes des conifères, sur les réponses électro-physiologiques et le comportement du scolyte Dendroctonus valens LeConte (Coleoptera : Curculionidae : Scolytinae), espèce introduite accidentellement en Chine depuis le milieu des années 1980. Tous les composés testés ont généré des réponses antennaires, dépendant de la dose. Dans des tests en olfactomètre en Y, on a observé une plus ou moins grande répulsion à certains composés. En particulier, les réponses au 1-octen-3-ol, au 1-hexanol, à l' hexanal et au 3-octanol ont été très faibles. Des expérimentations de terrain ont ensuite montré que l'attraction sur D. valens de pièges appâtés en kairomone a été réduite par l'adjonction de chacun des composés non-hôtes, cette réduction variant de 26.3 à 70 %. Parmi ces composés, le 1-octen-3-ol, le (Z)-3-hexen-1-ol et le (E)-2-hexen-1-ol ont été les plus efficaces, engendrant respectivement une réduction des captures de 69.5, 68.3 et 66.0 %. L'utilisation de composés volatils non-hôtes pour induire une confusion dans le choix de l'hôte représente donc un potentiel considérable pour le développement d'un programme de gestion des populations de D. valens, basé sur l'utilisation de produits semio-chimiques.scolyte / électro-antennogramme / réponse olfactive / composés volatiles non-hôtes / piégeages de terrain

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Behavioral Response of Lygus hesperus to Conspecifics and Headspace Volatiles of Alfalfa in a Y-Tube Olfactometer

Cited by 105 publications

References 17 publications

EAG-Active Herbivore-Induced Plant Volatiles Modify Behavioral Responses and Host Attack by An Egg Parasitoid

EAG-Active Herbivore-Induced Plant Volatiles Modify Behavioral Responses and Host Attack by An Egg Parasitoid

Production and Predator-Induced Release of Volatile Chemicals by the Plant Bug Lygus hesperus

Réponses électrophysiologiques et comportementales de Dendroctonus valens à des composés volatils non-hôtes

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