Behavioral phenotyping in rats during the experimental modeling of chronic cerebral circulation disorder

Куркин, Д. В.; Морковин, Е. И.; Verkholiak, D. V.; Osadchenko, N.A.; Tyurenkov, I. N.; Петров, В. И.

doi:10.17116/jnevro20171177169-73

Cited by 4 publications

(2 citation statements)

References 4 publications

(4 reference statements)

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“…The threads were tightened to fully arrest the blood flow. Then the nylon threads were alternately removed until the blood flow through the vessel resumed by 50% of the initial PLOS ONE values [13]. Blood flow in the common carotid arteries was recorded by the Doppler method.…”

Section: Experimental Model Of Chronic Brain Ischemiamentioning

confidence: 99%

Neuroprotective action of Cortexin, Cerebrolysin and Actovegin in acute or chronic brain ischemia in rats

et al. 2021

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This study was the first to compare the neuroprotective activity of Cerebrolysin®, Actovegin® and Cortexin® in rodent models of acute and chronic brain ischemia. The neuroprotective action was evaluated in animals with acute (middle cerebral artery occlusion) or chronic (common carotid artery stenosis) brain ischemia models in male rats. Cortexin® (1 or 3 mg/kg/day), Cerebrolysin® (538 or 1614 mg/kg/day) and Actovegin® (200 mg/kg/day) were administered for 10 days. To assess the neurological and motor impairments, open field test, adhesive removal test, rotarod performance test and Morris water maze test were performed. Brain damage was assessed macro- and microscopically, and antioxidant system activity was measured in brain homogenates. In separate experiments in vitro binding of Cortexin® to a wide panel of receptors was assessed, and blood-brain barrier permeability of Cortexin® was assessed in mice in vivo. Cortexin® or Cerebrolysin® and, to a lesser extent, Actovegin® improved the recovery of neurological functions, reduced the severity of sensorimotor and cognitive impairments in rats. Cortexin® reduced the size of necrosis of brain tissue in acute ischemia, improved functioning of the antioxidant system and prevented the development of severe neurodegenerative changes in chronic ischemia model. Radioactively labeled Cortexin® crossed the blood-brain barrier in mice in vivo with concentrations equal to 6–8% of concentrations found in whole blood. During in vitro binding assay Cortexin® (10 μg/ml) demonstrated high or moderate binding to AMPA-receptors (80.1%), kainate receptors (73.5%), mGluR1 (49.0%), GABAA1 (44.0%) and mGluR5 (39.7%), which means that effects observed in vivo could be related on the glutamatergic and GABAergic actions of Cortexin®. Thus, Cortexin, 1 or 3 mg/kg, or Cerebrolysin®, 538 or 1614 mg/kg, were effective in models acute and chronic brain ischemia in rats. Cortexin® contains compounds acting on AMPA, kainate, mGluR1, GABAA1 and mGluR5 receptors in vitro, and readily crosses the blood-brain barrier in mice.

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Section: Experimental Model Of Chronic Brain Ischemiamentioning

confidence: 99%

Neuroprotective action of Cortexin, Cerebrolysin and Actovegin in acute or chronic brain ischemia in rats

et al. 2021

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“…The choice of experimental animals is one of the key considerations in CAS modeling. Rats, rabbits and pigs are currently the most commonly used experimental animals for the study of CAS ( 42 – 44 ); however, due to their physiological and structural differences with humans, optimal efficacy often cannot be achieved. Cynomolgus monkeys are primates that are physiologically and structurally similar to humans; furthermore, they are easily fed, sensitive to a high-fat diet and readily develop CAS ( 45 ).…”

Section: Introductionmentioning

confidence: 99%

A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet

Zhang

Zeng

et al. 2018

Exp Ther Med

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Cardio-cerebrovascular disease is one of the three major causes of mortality in humans and constitutes a major socioeconomic burden. Carotid atherosclerosis (CAS) is a very common lesion of the arterial walls, which leads to narrowing of the arteries, in some cases occluding them entirely, increasing the risk of cardiovascular events. The aim of the present study was to evaluate a cynomolgus monkey model of carotid atherosclerosis (CAS) induced by puncturing and scratching combined with a high-fat diet. A total of 12 cynomolgus monkeys were randomly divided into four groups: A, puncturing and scratching carotid artery intimas + high-fat diet (n=3); B, puncturing and scratching carotid artery intimas + regular diet (n=3); C, high-fat diet only (n=3); and D, regular diet only (n=3). Blood was harvested at weeks 4, 6 and 8 and plasma lipid levels were assessed. At week 8, monkeys were sacrificed and carotid arteries were harvested for hematoxylin and eosin (H&E) staining to observe pathological changes. The results revealed that a high-fat diet led to increased plasma lipid levels and accelerated plaque formation. Carotid color Doppler ultrasonography was performed and, along with H&E staining, revealed plaque formation in group A. In summary, the results of the present study suggest that a cynomolgus monkey model of CAS model may be successfully constructed by puncturing and scratching of the carotid artery intimas in combination with a high-fat diet.

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Chronic isolated lightheadedness is a sign of abnormal plasma levels of phospholipids

Yao

et al. 2021

Experimental Gerontology

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Behavioral phenotyping in rats during the experimental modeling of chronic cerebral circulation disorder

Cited by 4 publications

References 4 publications

Neuroprotective action of Cortexin, Cerebrolysin and Actovegin in acute or chronic brain ischemia in rats

Neuroprotective action of Cortexin, Cerebrolysin and Actovegin in acute or chronic brain ischemia in rats

A cynomolgus monkey model of carotid atherosclerosis induced by puncturing and scratching of the carotid artery combined with a high‑fat diet

Chronic isolated lightheadedness is a sign of abnormal plasma levels of phospholipids

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