Behavioral Changes and Metabolic Responses of Adult Zebrafish (<i>Danio Rerio</i>) Exposed to Methamphetamine

Yin, Xingxing; Deng, Yanghui; Guo, Changsheng; Ding, Chunguang; Xu, Jian; Wu, Fengchang

doi:10.1021/acsestwater.3c00128

Cited by 3 publications

(5 citation statements)

References 47 publications

(71 reference statements)

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“…The toxic effects of MPs on aquatic vertebrates were mainly associated with oxidative damage [ 52 ] and thus induced inflammation in fish [ 55 ]. Moreover, METH exposure at low concentrations might induce oxidative stress and morphological developmental abnormalities in zebrafish [ 56 ]. Therefore, it should be determined whether the co-exposure of MPs (PS and PVC) and METH enhances the toxicity to zebrafish.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the same results were found in zebrafish larvae, including the simplified swimming trajectory, extended immobility, and lower mean velocity. Noticeably, exposure to METH showed the same anesthetic effects on zebrafish [ 56 ]. The addition of METH significantly alleviated the effects on the immobility duration of larvae induced by PS MPs while having no impact on the PVC group.…”

Section: Discussionmentioning

confidence: 99%

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The Exploration of Joint Toxicity and Associated Mechanisms of Primary Microplastics and Methamphetamine in Zebrafish Larvae

Wang,

Xu,

Yuan

et al. 2024

Toxics

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The co-existence of microplastics (MPs) and methamphetamine (METH) in aquatic ecosystems has been widely reported; however, the joint toxicity and associated mechanisms remain unclear. Here, zebrafish larvae were exposed individually or jointly to polystyrene (PS) and polyvinyl chloride (PVC) MPs (20 mg/L) and METH (1 and 5 mg/L) for 10 days. The mortality, behavioral functions, and histopathology of fish from different groups were determined. PS MPs posed a stronger lethal risk to fish than PVC MPs, while the addition of METH at 5 mg/L significantly increased mortality. Obvious deposition of MPs was observed in the larvae’s intestinal tract in the exposure groups. Meanwhile, treatment with MPs induced intestinal deposits and intestinal hydrops in the fish, and this effect was enhanced with the addition of METH. Furthermore, MPs significantly suppressed the locomotor activation of zebrafish larvae, showing extended immobility duration and lower velocity. METH stimulated the outcome of PS but had no effect on the fish exposed to PVC. However, combined exposure to MPs and METH significantly increased the turn angle, which declined in individual MP exposure groups. RNA sequencing and gene quantitative analysis demonstrated that exposure to PS MPs and METH activated the MAPK signaling pathway and the C-type lectin signaling pathway of fish, while joint exposure to PVC MPs and METH stimulated steroid hormone synthesis pathways and the C-type lectin signaling pathway in zebrafish, contributing to cellular apoptosis and immune responses. This study contributes to the understanding of the joint toxicity of microplastics and pharmaceuticals to zebrafish, highlighting the significance of mitigating microplastic pollution to preserve the health of aquatic organisms and human beings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Exploration of Joint Toxicity and Associated Mechanisms of Primary Microplastics and Methamphetamine in Zebrafish Larvae

Wang,

Xu,

Yuan

et al. 2024

Toxics

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“…Furthermore, METH did not significantly alter the levels of monoamine neurotransmitters in the zebrafish brain but did lead to reductions in L-tyrosine and L-carnitine [ 112 ]. L-tyrosine serves as a precursor for neurotransmitters such as dopamine, norepinephrine, epinephrine, and octopamine and is recognized as a key biomarker for amino acid metabolism, suggesting potential disruptions in this metabolic pathway within the fish brain.…”

Section: Bioaccumulation Of Illicit Drugs and Their Toxicitymentioning

confidence: 99%

“…Adverse effects of METH on the central nervous system of zebrafish indicate its potential to impact the endocrine system in these aquatic organisms, similar to other studied compounds [ 112 ]. The impact of these disruptions can be far-reaching, affecting various aspects of zebrafish biology, from behavior and metabolism to reproductive and developmental processes [ 84 , 112 , 134 , 142 , 143 ]. The endocrine regulatory effect of METH exacerbates through its impact on cognitive functioning, neurotransmitter systems, and metabolic pathways [ 144 ].…”

Section: Bioaccumulation Of Illicit Drugs and Their Toxicitymentioning

confidence: 99%

“…In terms of the immune system, there remains a lack of information regarding the effects of IDs on fish despite the significant sensitivity of immune-related parameters to both conventional and emerging pollutants [ 75 , 131 , 133 , 134 , 135 ]. Mentioned earlier alterations in fish brain metabolomes by environmental concentrations of METH, namely such specific metabolites as lyso-phosphatidylethanolamine, lyso-phosphatidylcholine, amino acids, carnitine, and long-chain fatty acids may be related to inflammatory responses [ 103 , 112 , 129 ]. As METH is a substrate of the dopamine transporter and taking into consideration the interconnectedness of the immune and nervous systems, it is plausible that IDs may exert immunomodulatory effects in fish through their impact on neurotransmitter systems and physiological processes [ 151 ].…”

Section: Bioaccumulation Of Illicit Drugs and Their Toxicitymentioning

confidence: 99%

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Illicit Drugs in Surface Waters: How to Get Fish off the Addictive Hook

Falfushynska,

Rychter,

Boshtova

et al. 2024

Pharmaceuticals

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The United Nations World Drug Report published in 2022 alarmed that the global market of illicit drugs is steadily expanding in space and scale. Substances of abuse are usually perceived in the light of threats to human health and public security, while the environmental aspects of their use and subsequent emissions usually remain less explored. However, as with other human activities, drug production, trade, and consumption of drugs may leave their environmental mark. Therefore, this paper aims to review the occurrence of illicit drugs in surface waters and their bioaccumulation and toxicity in fish. Illicit drugs of different groups, i.e., psychostimulants (methamphetamines/amphetamines, cocaine, and its metabolite benzoylecgonine) and depressants (opioids: morphine, heroin, methadone, fentanyl), can reach the aquatic environment through wastewater discharge as they are often not entirely removed during wastewater treatment processes, resulting in their subsequent circulation in nanomolar concentrations, potentially affecting aquatic biota, including fish. Exposure to such xenobiotics can induce oxidative stress and dysfunction to mitochondrial and lysosomal function, distort locomotion activity by regulating the dopaminergic and glutamatergic systems, increase the predation risk, instigate neurological disorders, disbalance neurotransmission, and produce histopathological alterations in the brain and liver tissues, similar to those described in mammals. Hence, this drugs-related multidimensional harm to fish should be thoroughly investigated in line with environmental protection policies before it is too late. At the same time, selected fish species (e.g., Danio rerio, zebrafish) can be employed as models to study toxic and binge-like effects of psychoactive, illicit compounds.

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Global occurrence, distribution, and ecological risk assessment of psychopharmaceuticals and illicit drugs in surface water environment: A meta-analysis

Zhang,

Guo,

et al. 2024

Water Research

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Behavioral Changes and Metabolic Responses of Adult Zebrafish (Danio Rerio) Exposed to Methamphetamine

Cited by 3 publications

References 47 publications

The Exploration of Joint Toxicity and Associated Mechanisms of Primary Microplastics and Methamphetamine in Zebrafish Larvae

The Exploration of Joint Toxicity and Associated Mechanisms of Primary Microplastics and Methamphetamine in Zebrafish Larvae

Illicit Drugs in Surface Waters: How to Get Fish off the Addictive Hook

Global occurrence, distribution, and ecological risk assessment of psychopharmaceuticals and illicit drugs in surface water environment: A meta-analysis

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