Behavioral and genomic impacts of a wastewater effluent on the fathead minnow

García-Reyero, Natàlia; Lavelle, Candice M.; Escalon, B. Lynn; Martinović, Dalma; Kroll, Kevin J.; Sorensen, Peter W.; Denslow, Nancy D.

doi:10.1016/j.aquatox.2010.08.014

Cited by 83 publications

(69 citation statements)

References 41 publications

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“…Transcriptional expression levels can be utilized to understand the functional network of the biochemical pathways they are involved in, and thus are useful in the characterization of biological processes of interest. Notable examples are those of Spromberg and Meador [97], who were able to associate immune suppression, reproductive dysfunction and somatic growth impairment in salmonids, to population level responses using life history models, Heckmann et al [98] and Connon et al [99] who were successful in combining molecular and organismal stress responses in Daphnia magna , by assessing mechanistic responses to exposure indicative of chronic consequences for population growth rates, and Garcia-Reyero et al [92,100] who investigated synergistic and antagonistic effect of EDC mixtures, identifying specific signatures relating to the modes of action of each chemical, and highlight the complexities of assessing multiple compound in wastewater treatment effluent. Studies of this kind emphasize the importance of bridging the assessment of toxicity responses at environmentally relevant (or low) concentrations that can mechanistically explain the effects contaminants may have at higher levels of biological organization and resulting population dynamics.…”

Section: Promising New Approaches and Toolsmentioning

confidence: 99%

Effect-Based Tools for Monitoring and Predicting the Ecotoxicological Effects of Chemicals in the Aquatic Environment

Connon

Geist

Werner

2012

Sensors

227

113

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Ecotoxicology faces the challenge of assessing and predicting the effects of an increasing number of chemical stressors on aquatic species and ecosystems. Herein we review currently applied tools in ecological risk assessment, combining information on exposure with expected biological effects or environmental water quality standards; currently applied effect-based tools are presented based on whether exposure occurs in a controlled laboratory environment or in the field. With increasing ecological relevance the reproducibility, specificity and thus suitability for standardisation of methods tends to diminish. We discuss the use of biomarkers in ecotoxicology including ecotoxicogenomics-based endpoints, which are becoming increasingly important for the detection of sublethal effects. Carefully selected sets of biomarkers allow an assessment of exposure to and effects of toxic chemicals, as well as the health status of organisms and, when combined with chemical analysis, identification of toxicant(s). The promising concept of “adverse outcome pathways (AOP)” links mechanistic responses on the cellular level with whole organism, population, community and potentially ecosystem effects and services. For most toxic mechanisms, however, practical application of AOPs will require more information and the identification of key links between responses, as well as key indicators, at different levels of biological organization, ecosystem functioning and ecosystem services.

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Section: Promising New Approaches and Toolsmentioning

confidence: 99%

Effect-Based Tools for Monitoring and Predicting the Ecotoxicological Effects of Chemicals in the Aquatic Environment

Connon

Geist

Werner

2012

Sensors

227

113

View full text Add to dashboard Cite

show abstract

“…Although environmental chemicals are usually less potent than endogenous hormones, it is now clear that they act additively with them (Markey et al, 2002). One important group among EDCs, found in the aquatic environment, are estrogens, both natural such as 17b-estradiol (E 2 ), estrone and estriol, and synthetic such as 17a-ethinylestradiol (EE 2 ) (Garcia-Reyero et al, 2011;Hinteman et al, 2006;Johnson et al, 2005;Ternes et al, 1999). EE 2 , a pharmacological compound with strong estrogenic activity, widely used as oral contraceptive and in hormone replacement therapy, is present in sewage treatment plant effluents in concentrations of 1e10 ng/L, although levels as high as 42 ng/L have been documented (Desbrow et al, 1988;Ternes et al, 1999), as it is poorly removed by conventional wastewater treatment methods (Karpova et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Tamoxifen persistently disrupts the humoral adaptive immune response of gilthead seabream (Sparus aurata L.)

Rodenas

Cabas

Abellán

et al. 2015

Developmental & Comparative Immunology

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“…Of these, 10 from each were found in the CRP, suggesting a similar pattern of gene regulation. The remainder of the genes either are unique to downstream (9) or effluent (7) or were found in both but with opposite gene regulation (8). This was a common trend throughout many of the significant KEGG pathways identified at the R site.…”

Section: Environmental Science and Technologymentioning

confidence: 89%

Using Transcriptomic Tools to Evaluate Biological Effects Across Effluent Gradients at a Diverse Set of Study Sites in Minnesota, USA

Berninger¹,

Martinović-Weigelt²,

García-Reyero³

et al. 2014

Environ. Sci. Technol.

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The aim of this study was to explore the utility of "omics" approaches in monitoring aquatic environments where complex, often unknown stressors make chemical-specific risk assessment untenable. We examined changes in the fathead minnow (Pimephales promelas) ovarian transcriptome following 4-day exposures conducted at three sites in Minnesota (MN, USA). Within each site, fish were exposed to water from three locations along a spatial gradient relative to a wastewater treatment plant (WWTP) discharge. After exposure, site-specific impacts on gene expression in ovaries were assessed. Using an intragradient point of comparison, biological responses specifically associated with the WWTP effluent were identified using functional enrichment analyses. Fish exposed to water from locations downstream of the effluent discharges exhibited many transcriptomic responses in common with those exposed to the effluent, indicating that effects of the discharge do not fully dissipate downstream. Functional analyses showed a range of biological pathways impacted through effluent exposure at all three sites. Several of those impacted pathways at each site could be linked to potential adverse reproductive outcomes associated with the hypothalamic−pituitary−gonadal (HPG) axis in female fathead minnows, specifically signaling pathways associated with oocyte meiosis, TGF-beta signaling, gonadotropin-releasing hormone (GnRH) and epidermal growth factor receptor family (ErbB), and gene sets associated with cyclin B-1 and metalloproteinase. The utility of this approach comes from the ability to identify biological responses to pollutant exposure, particularly those that can be tied to adverse outcomes at the population level and those that identify molecular targets for future studies.

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Behavioral and genomic impacts of a wastewater effluent on the fathead minnow

Cited by 83 publications

References 41 publications

Effect-Based Tools for Monitoring and Predicting the Ecotoxicological Effects of Chemicals in the Aquatic Environment

Effect-Based Tools for Monitoring and Predicting the Ecotoxicological Effects of Chemicals in the Aquatic Environment

Tamoxifen persistently disrupts the humoral adaptive immune response of gilthead seabream (Sparus aurata L.)

Using Transcriptomic Tools to Evaluate Biological Effects Across Effluent Gradients at a Diverse Set of Study Sites in Minnesota, USA

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