Behavioral and electrophysiological response of sugarcane aphid and imported fire ant to 2,4,6-trimethylpyridine, an alkaloid from the pink-spotted lady beetle

Zhou, Aiming; Du, Yuzhe; Riddick, Eric W.; Li, Lei; Chen, Jian

doi:10.1007/s10340-021-01363-6

Cited by 5 publications

(13 citation statements)

References 68 publications

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“…Underground nest excavating and tunnel digging are intrinsic behaviors of many ant species. , Digging and residing preference analysis was demonstrated to be a reliable method to determine the influence of chemicals on fire ant preference in previous studies. ,, In this study, 1-octen-3-ol significantly reduced the digging preference of fire ants at 1 μg/μL but enhanced both the digging and residing preference of fire ants at 10 –3 μg/μL (Figure A,E). The residing preference of fire ants was suppressed by 1-octen-3-ol at 10 –2 , 10 –1 , and 1 μg/μL (Figure E).…”

Section: Resultssupporting

confidence: 50%

“…To explore the volatile compounds from mealybug honeydew, headspace solid-phase microextraction (HS-SPME) was used to extract the volatiles from honeydew. Gray SPME fibers (DVB/CAR/PDMS, 50/30 μm) and manual holder (Supelco Inc., Bellefonte, PA) were used as in our previous study . About 20 μL of honeydew was collected and transferred into a 1 mL glass vial.…”

Section: Methodsmentioning

confidence: 99%

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Semiochemicals Produced by Microbes in Mealybug Honeydew Attract Fire Ants

Zhang,

Fu,

Shao

et al. 2023

J. Agric. Food Chem.

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Semiochemicals produced by microbes in hemipteran honeydew play an important role in mediating the ant–hemipteran interaction. However, the specific volatile compounds and their origins remain unclear. Here, we found that honeydew produced by Phenacoccus solenopsis exhibited strong attractiveness to fire ant workers, but sterilized honeydew was much less attractive. Four compounds were identified from the honeydew volatiles, including 1-octen-3-ol, limonene, phenylethyl alcohol, and 2,4-ditert-butylphenol. All the identified compounds triggered strong electroantennography response of fire ants and showed repellence at relatively high concentrations while attractiveness at low concentrations to fire ant workers. Furthermore, six bacterial isolates were identified based on 16S rRNA sequencing, namely, Bacillus, Brachybacterium, Kocuria, Microbacterium, Pseudomonas, and Staphylococcus. Fire ants exhibited a strong preference for Brachybacterium, Kocuria, and Microbacterium, suggesting that these bacterial isolates are involved in the attracting effect of honeydew. Both limonene and phenylethyl alcohol were detected from Brachybacterium, while limonene was only detected from Kocuria, and phenylethyl alcohol was exclusively detected from Microbacterium. Reinoculation of these bacteria restored the attractiveness of honeydew to fire ants, and the active compounds, limonene and phenylethyl alcohol, were detectable in bacteria-reinoculated honeydew. Collectively, our results reveal the active compounds in hemipteran honeydew and their association with honeydew bacteria. The findings will contribute to the development of novel attractants for efficient monitoring of fire ants.

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Section: Resultssupporting

confidence: 50%

Section: Methodsmentioning

confidence: 99%

Semiochemicals Produced by Microbes in Mealybug Honeydew Attract Fire Ants

Zhang,

Fu,

Shao

et al. 2023

J. Agric. Food Chem.

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“…The alkaloid, 2,4,6-trimethylpyridine, from the lady beetle, was previously demonstrated to evoke strong EAG responses in S. invicta. 14,31 However, exposure to 100 mg Cd kg −1 for 2 weeks deprived the EAG response to this odorant. 27 Analogously, the electro-olfactogram (EOG) responses of goldfish to food-related odorants and postovulatory pheromone were weakened after 10 min exposure to 0.1 mM copper sulfate.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Despite high levels of omnivory, S. invicta is frequently engaged in mutualisms in which they consume plant-based foods including nectar, extrafloral nectar, and honeydew produced by hemipterans . Carbohydrate-rich plant-derived food resources can greatly promote the brood production and colony growth of S. invicta . , We recently reported that S. invicta closely associates with sugar cane aphids Melanaphis sacchari and consumes honeydew produced by M. sacchari . Sorghum ( Sorghum bicolor ), an important host plant of M. sacchari , has great potential in phytoremediation of Cd-polluted soils and can absorb and accumulate 10 times the Cd concentration from soil and poison consumers higher in the food chain .…”

Section: Methodsmentioning

confidence: 99%

“…The semiochemicals used in this assay were obtained from honeydew produced by sugar cane aphids Melanaphis sacchari that could attract fire ant workers (unpublished data) and one alkaloid, 2,4,6-trimethylpyridine (TMP), from the pink-spotted lady beetle Coleomegilla maculata . 2,4,6-Trimethylpyridine had been found to have strong electrophysiological and behavioral activities with fire ant workers in our previous studies. , Ant workers from colonies without Cd exposure were used for RNA extraction. The detailed procedures for protein function determination, including cDNA cloning, vector plasmid construction, recombinant protein expression and purification, and the binding affinity evaluation of SiOBPs, are respectively elaborated in Text S8, Text S9, and Text S10 based on previous studies. , …”

Section: Methodsmentioning

confidence: 99%

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Sublethal Exposure to Cadmium Induces Chemosensory Dysfunction in Fire Ants

Yang

Zhang

Liu

et al. 2022

Environ. Sci. Technol.

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Ants easily accumulate cadmium (Cd) from the food web in terrestrial ecosystems. Cd contamination may cause olfactory dysfunction and consequently disorders in the social behavior of ants. To explore the molecular mechanism underlying the effect of Cd exposure on the chemosensory process of ants, we characterized the Cd-induced variations in the expression of genes involved in chemoreception and electrophysiological and behavioral sensitivity to semiochemicals by using the red imported fire ant, Solenopsis invicta, as a model system. As a result, Cd exposure increased Cd accumulation and decreased the survival rate of S. invicta. Cd exposure altered the expression profiles of odor binding protein genes of S. invicta (SiOBPs). Specifically, SiOBP15 protein expression was upregulated upon Cd exposure. Both SiOBP7 and SiOBP15 exhibited high binding affinities to limonene, nonanal, and 2,4,6-trimethylpyridine. S. invicta exposed to Cd showed less sensitive electrophysiological and behavioral response to the three chemicals but exhibited sensitive perception to undecane. Silencing of SiOBP7 and SiOBP15 abolished the behavioral response of S. invicta to nonanal and undecane, respectively, suggesting that SiOBP7 and SiOBP15 play essential roles in the chemoreception of S. invicta. In general, our results suggest that Cd contamination may interfere with olfactory signal transduction by altering the expression of SiOBPs, consequently evoking chemosensory dysfunction in fire ants.

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Bioactivities of scent gland chemicals from Mictis fuscipes Hsiao (Hemiptera: Coreidae) on Solenopsis invicta Buren (Hymenoptera: Formicidae)

Zhong,

Fox,

Ling

et al. 2024

Pest Management Science

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BACKGROUNDGiven the chemical diversity within stink bugs scent glands, they can be convenient models for bioprospecting novel pest control products. Preliminary behaviour observations indicated that adult Mictis fuscipes stink bugs secrete liquid droplets when defending against Solenopsis invicta fire ants, killing them within minutes. Hence, this study aimed to analyse the chemical composition of the metathoracic scent gland secretions of M. fuscipes adults, as well as assess their biological activities against fire ants.RESULTSBioassaying fire ants against secretions of several local stink bugs confirmed that the defensive secretions of two Mictis species are significantly more lethal, where M. fuscipes was the most lethal. Volatiles chromatography analysis indicated the secretions of female and male M. fuscipes stink bugs contains 20 and 26 components, respectively, chiefly hexanoic acid and hexyl hexanoate. Five compounds were consistently present in the secretion of female adults: hexyl hexanoate, hexanoic acid, hexyl acetate, hexyl butyrate, and eugenol. These yielded a strong electrophysiological antennal (EAD) response from S. invicta workers, female alates and males, where hexyl acetate showed the strongest response. The combination of these five compounds proved strongly repellent to S. invicta. When tested singly, hexanoic acid, hexyl butyrate, hexyl hexanoate, and eugenol were repellent to S. invicta, but hexyl acetate seemed slightly attractive. Additionally, the same mixture of five components exhibited strong contact and fumigant toxicity towards S. invicta workers, eugenol being the strongest.CONCLUSIONDefensive chemicals of M. fuscipes exhibit robust biological activity against S. invicta and could inspire the development of biopesticides. © 2024 Society of Chemical Industry.

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Behavioral and electrophysiological response of sugarcane aphid and imported fire ant to 2,4,6-trimethylpyridine, an alkaloid from the pink-spotted lady beetle

Cited by 5 publications

References 68 publications

Semiochemicals Produced by Microbes in Mealybug Honeydew Attract Fire Ants

Semiochemicals Produced by Microbes in Mealybug Honeydew Attract Fire Ants

Sublethal Exposure to Cadmium Induces Chemosensory Dysfunction in Fire Ants

Bioactivities of scent gland chemicals from Mictis fuscipes Hsiao (Hemiptera: Coreidae) on Solenopsis invicta Buren (Hymenoptera: Formicidae)

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