Behavioral and accumbens synaptic plasticity induced by cues associated with restraint stress

García‐Keller, Constanza; Carter, Jordan S.; Kruyer, Anna; Kearns, Angela; Hopkins, Jordan L; Hodebourg, Ritchy; Kalivas, Peter W.; Reichel, Carmela M.

doi:10.1038/s41386-021-01074-7

Cited by 18 publications

(12 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Presentation of the stress CS was sufficient to induce heroin-seeking in drug-treated rats. We have also shown that presentation of the stress CS dysregulates coping strategies in a defensive burying task (Carter et al, 2020;Garcia-Keller et al, 2021) increases corticosterone, and potentiates maladaptive plasticity in the nucleus accumbens core (Garcia-Keller et al, 2021). Importantly, these responses did not involve exposure to the primary stressor, but to a CS or "trigger" associated with the original stressor.…”

Section: Introductionmentioning

confidence: 82%

“…The presence of the heroin-associated cue surpassed any effects of the CS or NS on responding. Reinstatement to stress associated cues appears to be drug specific because the stress CS has reinstated heroin, alcohol, and cocaine (Garcia-Keller et al, 2019;Carter et al, 2020) but not sucrose (Garcia-Keller et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Rats underwent a single restraint stress episode or were sham treated. Details are provided in Supplementary Material and previously published methods (Carter et al, 2020;Garcia-Keller et al, 2021). A schematic representation of the stress protocol is in Figure 1A.…”

Section: Restraint Stress and Scent Exposurementioning

confidence: 99%

“…We have previously shown that stress rats adopt an avoidant coping strategy in the presence of stress cues (Carter et al, 2020;Garcia-Keller et al, 2021). The goal of this experiment was to determine patterns of compartment placement, activity, and immobility in response to the stress CS or an NS during reinstatement testing.…”

Section: Experiments 2: Behavioral Patterns In Response To a Stress Conditioned Stimulus During Reinstatement Testingmentioning

confidence: 99%

See 3 more Smart Citations

Complex Interactions Between Sex and Stress on Heroin Seeking

2021

Self Cite

View full text Add to dashboard Cite

Rationale: Stress plays a dual role in substance use disorders as a precursor to drug intake and a relapse precipitant. With heroin use at epidemic proportions in the United States, understanding interactions between stress disorders and opioid use disorder is vital and will aid in treatment of these frequently comorbid conditions.Objectives: Here, we combine assays of stress and contingent heroin self-administration (SA) to study behavioral adaptations in response to stress and heroin associated cues in male and female rats.Methods: Rats underwent acute restraint stress paired with an odor stimulus and heroin SA for subsequent analysis of stress and heroin cue reactivity. Lofexidine was administered during heroin SA and reinstatement testing to evaluate its therapeutic potential. Rats also underwent tests on the elevated plus maze, locomotor activity in a novel environment, and object recognition memory following stress and/or heroin.Results: A history of stress and heroin resulted in disrupted behavior on multiple levels. Stress rats avoided the stress conditioned stimulus and reinstated heroin seeking in response to it, with males reinstating to a greater extent than females. Lofexidine decreased heroin intake, reinstatement, and motor activity. Previous heroin exposure increased time spent in the closed arms of an elevated plus maze, activity in a round novel field, and resulted in object recognition memory deficits.Discussion: These studies report that a history of stress and heroin results in maladaptive coping strategies and suggests a need for future studies seeking to understand circuits recruited in this pathology and eventually help develop therapeutic approaches.

show abstract

Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Restraint Stress and Scent Exposurementioning

confidence: 99%

Section: Experiments 2: Behavioral Patterns In Response To a Stress Conditioned Stimulus During Reinstatement Testingmentioning

confidence: 99%

See 2 more Smart Citations

Complex Interactions Between Sex and Stress on Heroin Seeking

2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Anxiety was produced by restraining mice in tubes ( Garcia-Keller et al, 2021 ). Briefly, mice were placed into a modified 50-mL Falcon tube with breather holes with only the tail and tip of the nose free for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Dissection of the relationship between anxiety and stereotyped self-grooming using the Shank3B mutant autistic model, acute stress model and chronic pain model

Liu

Huang

et al. 2021

Neurobiology of Stress

View full text Add to dashboard Cite

Repeated binge‐like eating episodes in female rats alter adenosine A_2A and dopamine D2 receptor genes regulation in the brain reward system

Mercante,

Micioni Di Bonaventura,

Pucci

et al. 2024

Intl J Eating Disorders

View full text Add to dashboard Cite

ObjectiveBinge‐eating disorder is an eating disorder characterized by recurrent binge‐eating episodes, during which individuals consume excessive amounts of highly palatable food (HPF) in a short time. This study investigates the intricate relationship between repeated binge‐eating episode and the transcriptional regulation of two key genes, adenosine A2A receptor (A2AAR) and dopamine D2 receptor (D2R), in selected brain regions of rats.MethodBinge‐like eating behavior on HPF was induced through the combination of food restrictions and frustration stress (15 min exposure to HPF without access to it) in female rats, compared to control rats subjected to only restriction or only stress or none of these two conditions. After chronic binge‐eating episodes, nucleic acids were extracted from different brain regions, and gene expression levels were assessed through real‐time quantitative PCR. The methylation pattern on genes' promoters was investigated using pyrosequencing.ResultsThe analysis revealed A2AAR upregulation in the amygdala and in the ventral tegmental area (VTA), and D2R downregulation in the nucleus accumbens in binge‐eating rats. Concurrently, site‐specific DNA methylation alterations at gene promoters were identified in the VTA for A2AAR and in the amygdala and caudate putamen for D2R.DiscussionThe alterations on A2AAR and D2R genes regulation highlight the significance of epigenetic mechanisms in the etiology of binge‐eating behavior, and underscore the potential for targeted therapeutic interventions, to prevent the development of this maladaptive feeding behavior. These findings provide valuable insights for future research in the field of eating disorders.Public SignificanceUsing an animal model with face, construct, and predictive validity, in which cycles of food restriction and frustration stress evoke binge‐eating behavior, we highlight the significance of epigenetic mechanisms on adenosine A2A receptor (A2AAR) and dopamine D2 receptor (D2R) genes regulation. They could represent new potential targets for the pharmacological management of eating disorders characterized by this maladaptive feeding behavior.

show abstract

Behavioral and accumbens synaptic plasticity induced by cues associated with restraint stress

Cited by 18 publications

References 54 publications

Complex Interactions Between Sex and Stress on Heroin Seeking

Complex Interactions Between Sex and Stress on Heroin Seeking

Dissection of the relationship between anxiety and stereotyped self-grooming using the Shank3B mutant autistic model, acute stress model and chronic pain model

Repeated binge‐like eating episodes in female rats alter adenosine A_2A and dopamine D2 receptor genes regulation in the brain reward system

Contact Info

Product

Resources

About

Behavioral and accumbens synaptic plasticity induced by cues associated with restraint stress

Cited by 18 publications

References 54 publications

Complex Interactions Between Sex and Stress on Heroin Seeking

Complex Interactions Between Sex and Stress on Heroin Seeking

Dissection of the relationship between anxiety and stereotyped self-grooming using the Shank3B mutant autistic model, acute stress model and chronic pain model

Repeated binge‐like eating episodes in female rats alter adenosine A2A and dopamine D2 receptor genes regulation in the brain reward system

Contact Info

Product

Resources

About

Repeated binge‐like eating episodes in female rats alter adenosine A_2A and dopamine D2 receptor genes regulation in the brain reward system