Behavior of the accessory body of Cajal during axon reaction

Haggar, Robert A.

doi:10.1002/cne.901080206

Cited by 18 publications

(4 citation statements)

References 5 publications

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“…On the contrary, the number of CBs decreases in supraoptic neurons when transcriptional activity is dramatically inhibited during the early neuronal response to osmotic stress (Lafarga et al, 1998). Additional data supporting the dynamic nature of CBs come from the observation that the disruption of transcription and rRNA processing induced by axotomy is associated with a substantial reduction in the number of CBs (Haggar, 1957; Clark et al, 1991). This reduced incidence of CBs after axotomy is accompanied by nucleolar changes that are similar to those observed during experimental rRNA down‐regulation in other cell types (Clark et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Neuronal body size correlates with the number of nucleoli and Cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons

et al. 2000

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Trigeminal ganglion neurons comprise three main cell body-size types. This cell size heterogeneity provides an excellent neuronal model to study the cell size-dependent organization and dynamics of the nucleoli, Cajal (coiled) bodies (CBs), and nuclear speckles of pre-mRNA splicing factors, nuclear structures that play a key role in the normal neuronal physiology. We have analyzed the number of nucleoli and CBs and the structural and molecular organization of CBs and nuclear speckles in the three neuronal types by using immunofluorescence with antibodies that recognize nucleoli (fibrillarin), CBs (coilin), and nuclear speckles (snRNPs), confocal microscopy, and electron microscopy. Whereas the mean number of nucleoli per neuron decreases as a function of cell size, the number of CBs per cell significantly increases in large neurons in comparison with the small ones. In addition, large neurons have a higher proportion of CBs associated with the nucleolus. In all neuronal types, CBs concentrate coilin, fibrillarin, snRNPs, and the survival motor neuron protein (SMN). Immunostaining for snRNPs shows small speckle domains and extensive areas of diffuse nucleoplasmic signal in large neurons, in contrast with the large nuclear speckles found in small neurons. Furthermore, flow cytometric analysis shows that all neurons are in the range of diploid cells. These findings indicate that the fusion behavior of nucleoli, the formation of CBs and their relationships with the nucleolus, as well as the compartmentalization of the pre-mRNA splicing machinery, is related to cell body size in the trigeminal ganglion neurons. Because transcriptional activity is a basic determinant mechanism of cell size in diploid cells, we suggest that our findings reflect a distinct transcription-dependent organization of the nucleolus and splicing machinery in the three cell types of trigeminal ganglion neurons.

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Section: Discussionmentioning

confidence: 99%

Neuronal body size correlates with the number of nucleoli and Cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons

et al. 2000

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“…Stimulated by the demonstration of the sex chromatin (later identified as the inactive X chromosome) in mammalian female nuclei, Barr's team published three papers (Haggar 1957;Nayyar and Barr 1968;Thompson et al 1957) on the organization of the accessory body in feline neurons using the silver nitrate modification of Nauta and Gyrax and the cytochemical method of Feulgen (for DNA). The authors confirmed Cajal's observations, performed a quantitative analysis of the incidence of accessory bodies in several nervous centers, reported the infrequent appearance, in feline neurons, of an argyrophilic paranuclear structure, which may be a precursor of the accessory body free in the nucleoplasm, and demonstrated that, unlike the sex chromatin, the accessory body is Feulgen negative.…”

Section: The Accessory Body ("Cajal Body")mentioning

confidence: 99%

Cajal’s contribution to the knowledge of the neuronal cell nucleus

et al. 2009

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In 1906, the Spanish neurobiologist Santiago Ramón y Cajal was awarded the Nobel Prize in Physiology or Medicine in recognition of his work on the structure of neurons and their connections. Cajal is commonly regarded as the father of modern neuroscience. What is less well known is that Cajal also had a great interest in intracellular neuronal structures and developed the reduced silver nitrate method for the study of neurofibrils (neurofilaments) and nuclear subcompartments. It was in 1903 that Cajal discovered the "accessory body" ("Cajal body") and seven years later, published an article on the organization of the cell nucleus in mammalian neurons that represents a masterpiece of nuclear structure at the light microscopy level. In addition to the accessory body, it includes the analysis of several nuclear components currently recognized as fibrillar centers of the nucleolus, nuclear speckles of splicing factors, transcription foci, nuclear matrix, and the double nuclear membrane. The aim of this article is to revisit Cajal's contributions to the knowledge of the neuronal nucleus in light of our current understanding of nuclear structure and function.

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“…Barr's team confirmed Cajal's observations in feline neurons and, with the cytochemical method of Feulgen, they demonstrated that the accessory body, unlike the sex chromatin, is relatively DNA poor. [17][18][19] It was not until 1969 that the first observation of the accessory body was made in non-neuronal tissue. By electron microscopy, Monneron and Bernhard (1969) reported a nuclear structure composed of dense coiled threads in hepatocytes and gave it the name of "coiled body" (Fig.…”

Section: 99mentioning

confidence: 99%