Behavior of nucleolus in the tobacco male meiocytes involved in cytomixis

Mursalimov, Sergey; Sidorchuk, Yuriy V.; Дейнеко, Е. В.

doi:10.1002/cbin.10718

Cited by 3 publications

(4 citation statements)

References 12 publications

(13 reference statements)

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“…Cytomixis has been reported to occur along cytomictic channels [26,27,41,42], and cytomixis during the prophase of the first meiotic division is widespread in plant species and is evolutionarily important . CC-BY 4.0 International license perpetuity.…”

Section: Discussionmentioning

confidence: 99%

“…It is reported that irregular cytomixis may be caused by various disturbances such as the structure of the cytomictic channels, synthesis of the callose coat, cell wall formation, the structure of the cell cytoskeleton and genetic unbalance [27,42]. As has been reviewed, the microtubular apparatus does not play an obvious role in the process of cytomixis in tobacco [26,42,47,48]. Furthermore, asymmetrical deposition of the callose wall occurred at similar ratio at the zygotene of meiosis I in the PMCs of both the F1-S fertile and F1-D sterile hybrids.…”

Section: Discussionmentioning

confidence: 99%

“…Cell membrane expansion at the unicellular microspore stage is vital for pollen cell enlargement, polarization, mitosis, and finally pollen viability [1]. In addition, the timing of tapetum formation and degradation is critical for microspore development and pollen viability; premature or delayed tapetum degeneration causes male sterility [25,26]. Aberrant pollen wall formation might also lead to pollen abortion.…”

Section: Introductionmentioning

confidence: 99%

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Cytological abnormalities during pollen development in interspecific hybrids of Nicotiana

et al. 2020

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Nicotiana alata is resistant to Tomato spotted wilt virus (TSWV) and of great value in breeding. However, hybrid sterility constrains the application of interspecific genetic resources. Previously, we obtained interspecific hybrids between a cytoplasmic male sterility (CMS) line of Nicotiana tabacum and N. alata, some of which were pollen sterile. In the present research, we studied the cytological abnormalities during pollen development in sterile hybrids (F1-D) by comparing pollen development with that in fertile hybrids (F1-S) from the same cross. Transmission electron microscopy and DiI staining showed that the membrane structures of microspores and pollen in F1-D sterile hybrids were impaired. Carbol fuchsin staining revealed that cytomixis, chromosome loss and asymmetric callose wall formation occurred with high frequency in the microsporocytes and microspores of the sterile hybrids. The cytoplasm and nucleus were lost in the microspores and pollen of sterile hybrids, leading to mature pollen grains that were vacuous and collapsed in the aperture region. In addition, delayed tapetum degradation was detected in the anther of sterile hybrids, and sporopollenin was deposited in the aperture region. Impaired membrane structures of microspores and pollen in F1-D sterile hybrids affected the integrity of the cells, and might be associated with chromosome, nuclear and cytoplasm loss, vacuous pollen, and sterility in F1-D hybrids. Abnormal tapetum degradation in the anther and irregular sporopollenin deposition in the pollen wall of the F1-D sterile hybrids might also be related to the pollen sterility. This study deepens our understanding of the cytological mechanisms of hybrid sterility, and may facilitate the application of TSWV-resistant resources in cultivated Nicotiana species through hybrid fertility restoration and backcross breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytological abnormalities during pollen development in interspecific hybrids of Nicotiana

et al. 2020

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“…The migrating chromatin displays no signs of inactivation or damage and, most likely, is represented by Analysis of cytoskeleton in the cells involved in cytomixis: the migrated chromatin displays an MT-organizing activity and can interact with the spindle Institute of Molecular Biology, Slovak Academy of Sciences 2019 whole chromosomes. Cells involved in cytomixis also show no signs of programmed cell death (Mursalimov et al , 2017. See the relevant reviews for more comprehensive information (Lone and Lone 2013;Mandal et al 2013;Mursalimov and Deineko 2018).…”

Section: Introductionmentioning

confidence: 99%

Analysis of cytoskeleton in the cells involved in cytomixis: the migrated chromatin displays an MT-organizing activity and can interact with the spindle

2019

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The structure of cytoskeleton in the tobacco male meiocytes involved in the migration of nuclei between cells (cytomixis) is studied. The tubulin and actin components of cytoskeleton are examined using specific antibodies and phalloidin. The presence of microtubules and actin filaments inside cytomictic channels directly when the nuclei migrate through these channels is demonstrated for the first time. The actin and tubulin cytoskeleton is shown to retain its reticular structure in early prophase I before the beginning of cytomixis, during nuclear migration, and after completion of this process and emergence of micronuclei in the cytoplasm of recipient cells. It is demonstrated that if migration takes place in late prophase I, the nucleus leaves the perinuclear tubulin cage, encompassing it at this stage. After migration, the cytomictic micronuclei in recipient cell reside beyond the perinuclear tubulin cage in both meiotic prophase I and II. A microtubule-organizing activity of the migrated chromatin in meta-telophase I and II is demonstrated for the first time as well as the ability of this chromatin to form independent minispindles and radial microtubule arrays and contact the spindle of recipient cell. The role of cytomixis in production of aneuploid and unreduced pollen is discussed.

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Cytological abnormalities and pollen abortion in interspecific hybrids ofNicotiana

Liao

Chen

Pan

et al. 2019

Preprint

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Interspecific cross breeding introduces superior agronomic traits into cultivated species; 12 however, problematic pollen sterility occurs in the hybrids. Our previous study obtained interspecific 13 hybrids from the cross between a cytoplasmic male sterility line of Nicotiana tabacum and Nicotiana 14 alata, and some of the hybrids were pollen sterile. Here, we conducted an in-depth cellular study to 15 understand the cytological mechanism of pollen abortion in these hybrids (F1-D sterile) compared with 16 pollen development in the fertile hybrids (F1-S sterile) from the same cross. The ultrastructure 17 observation showed that the membrane of microspore in F1-D sterile hybrid was deficient at all 18 represented developmental stages. Chromosome behavior during meiosis was studied by carbol fuchsin 19 staining, which indicated that cytomixis, chromosome leakage and asymmetric callose wall deposition 20 occurred with high frequency in the microsporocyte of F1-D sterile. The results of the ultrastructure and 21 6-diamidino-2-phenylindole (DAPI) analyses also showed that the cytoplasm and nucleus were unstable 22 and extruded from F1-D sterile microspore during the developmental process, leading to mature pollen 2 1 grains that were vacuous and collapsed in the aperture region. In addition, delayed tapetum degradation 2 was also detected in the anther of F1-D sterile, and might be associated with irregular sporopollenin 3 deposition in the aperture region of F1-D sterile pollen. Genetic unbalance and cytomembrane deficiency 4 might both be responsible for the instability of the chromosome, nuclear and cytoplasm, and resulted in 5 pollen abortion in F1-D sterile hybrids, and irregular tapetum degradation might also be related with 6 pollen sterility. 7

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Behavior of nucleolus in the tobacco male meiocytes involved in cytomixis

Cited by 3 publications

References 12 publications

Cytological abnormalities during pollen development in interspecific hybrids of Nicotiana

Cytological abnormalities during pollen development in interspecific hybrids of Nicotiana

Analysis of cytoskeleton in the cells involved in cytomixis: the migrated chromatin displays an MT-organizing activity and can interact with the spindle

Cytological abnormalities and pollen abortion in interspecific hybrids ofNicotiana

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