Behavior of mammalian cells on magnesium substituted bare and hydroxyapatite deposited (Ti,Mg)N coatings

Onder, Sakip; Calikoglu-Koyuncu, Ayse Ceren; Kazmanlı, Kürşat; Ürgen, Mustafa; Köse, Gamze Torun; Kök, Fatma Neşe

doi:10.1016/j.nbt.2014.11.006

Cited by 13 publications

(5 citation statements)

References 51 publications

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“…μCT analysis also showed that local Mg presence resulted a significant increase on bone mineral density at the fracture site while it has no significant effect on the mineral density in the callus (Figure 6). These results are consistent with the experiments carried out in vitro, 40,41 stating the positive effect of Mg on mineralization. It has been reported that bone strength increases with bone mineral density and there is 80% correlation between BMD and bone strength 47,48 .…”

Section: Discussionsupporting

confidence: 92%

“…Currently, FDA approved TiN coatings are preferred to decrease the friction and increase the wear resistance of Ti based materials in different orthopedic applications such as joint replacement, long bone fractures, and in dental applications as bone pins. [36][37][38] In order to increase the osteointegration of these implants, Mg doped TiN, that is, (Ti,Mg)N, coatings were previously developed and characterized by our group and <10 at% Mg doping was chosen for its high corrosion resistance, 39,40 enhanced mineralization [39][40][41][42] and cell differentiation 43 performance. In the present study, in vivo performance of this coating was demonstrated in rabbit femur fracture models.…”

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confidence: 99%

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Assessment of bone healing using (Ti,Mg)N thin film coated plates and screws: Rabbit femur model

et al. 2020

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Magnesium (Mg) based implants such as plates and screws are often preferred to treat bone defects because of the positive effects of magnesium in bone growth and healing. Their low corrosion resistance, however, leads to fast degradation and consequently failure before healing was completed. Previously, we developed Mg doped titanium nitrate (TiN) thin film coatings to address these limitations and demonstrated that <10 at% Mg doping led to enhanced mineralization in vitro. In the present study, in vivo performance of (Ti,Mg)N coated Ti6Al4V based plates and screws were studied in the rabbit model. Bone fractures were formed on femurs of 16 rabbits and then fixed with either (Ti,Mg)N coated (n = 8) or standard TiN coated (n = 8) plates and screws. X-ray imaging and μCT analyses showed enhanced bone regeneration on fracture sites fixed with (Ti,Mg)N coated plates in comparison with the Mg free ones. Bone mineral density, bone volume, and callus volume were also found to be 11.4, 23.4, and 42.8% higher, respectively, in accordance with μCT results. Furthermore, while TiN coatings promoted only primary bone regeneration, (Ti,Mg)N led to secondary bone regeneration in 6 weeks. These results indicated that Mg presence in the coatings accelerated bone regeneration in the fracture site. (Ti,Mg)N coating can be used as a practical method to increase the efficiency of existing bone fixation devices of varying geometry.

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Section: Discussionsupporting

confidence: 92%

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confidence: 99%

Assessment of bone healing using (Ti,Mg)N thin film coated plates and screws: Rabbit femur model

et al. 2020

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“…Interestingly, most of the coatings are based on (calcium) phosphate compounds and are thus not suitable for combination with a magnesium-based barrier membrane due to their brittleness and inflexibility [ 7 , 9 , 10 ]. An appropriate alternative method is coatings made via physical vapor deposition (PVD), which produces thin films on the surface of biomaterials [ 7 , 11 , 12 ]. This coating technique is mainly used to improve the optical, mechanical or tribological characteristics of different materials such as surgical instruments [ 7 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…An appropriate alternative method is coatings made via physical vapor deposition (PVD), which produces thin films on the surface of biomaterials [ 7 , 11 , 12 ]. This coating technique is mainly used to improve the optical, mechanical or tribological characteristics of different materials such as surgical instruments [ 7 , 11 , 12 ]. The technique ion implantation (II) includes the bombardment of ionized species and their implantation into the first atomic layers of a solid and can be combined with different other coating techniques [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility and Immune Response of a Newly Developed Volume-Stable Magnesium-Based Barrier Membrane in Combination with a PVD Coating for Guided Bone Regeneration (GBR)

Steigmann

Jung

Kieferle³

et al. 2020

Biomedicines

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To date, there are no bioresorbable alternatives to non-resorbable and volume-stable membranes in the field of dentistry for guided bone or tissue regeneration (GBR/GTR). Even magnesium (Mg) has been shown to constitute a favorable biomaterial for the development of stabilizing structures. However, it has been described that it is necessary to prevent premature degradation to ensure both the functionality and the biocompatibility of such Mg implants. Different coating strategies have already been developed, but most of them did not provide the desired functionality. The present study analyses a new approach based on ion implantation (II) with PVD coating for the passivation of a newly developed Mg membrane for GBR/GTR procedures. To demonstrate comprehensive biocompatibility and successful passivation of the Mg membranes, untreated Mg (MG) and coated Mg (MG-Co) were investigated in vitro and in vivo. Thereby a collagen membrane with an already shown biocompatibility was used as control material. All investigations were performed according to EN ISO 10993 regulations. The in vitro results showed that both the untreated and PVD-coated membranes were not cytocompatible. However, both membrane types fulfilled the requirements for in vivo biocompatibility. Interestingly, the PVD coating did not have an influence on the gas cavity formation compared to the uncoated membrane, but it induced lower numbers of anti-inflammatory macrophages in comparison to the pure Mg membrane and the collagen membrane. In contrast, the pure Mg membrane provoked an immune response that was fully comparable to the collagen membrane. Altogether, this study shows that pure magnesium membranes represent a promising alternative compared to the nonresorbable volume-stable materials for GBR/GTR therapy.

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“…HA is an ideal material to design bioactive drug carrier systems and coatings on implant surfaces due to its osteogenic activity 33–36. Nano sized HA (nHA) had also been shown to have antimicrobial properties, so it can be used in the design of antimicrobial surfaces 36.…”

Section: Introductionmentioning

confidence: 99%

A functional coating to enhance antibacterial and bioactivity properties of titanium implants and its performance in vitro

et al. 2020

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Bacterial infections and lack of osseointegration may negatively affect the success of titanium (Ti) implants. In the present study, a functional coating composed of chitosan (CS) microspheres and nano hydroxyapatite (nHA) was prepared to obtain antimicrobial Ti implants with enhanced bioactivity. First, the chitosan microspheres were fixed to Ti surfaces activated by alkali and heat treatment, then nHA coatings were precipitated onto these surfaces. Ciprofloxacin was loaded into the microspheres using two different procedures; encapsulation and diffusion. Scanning electron microscopy micrographs of the modified Ti surfaces showed that the coating was successfully deposited onto the Ti surfaces and stable for 30 days in PBS. The drug was completely released from free microspheres loaded by encapsulation in 21 days whereas only 89% release was observed after immobilization. The burst release also decreased from ca. 55% to ca. 35%. The release was further reduced following the nHA precipitation. The modified Ti surfaces showed antimicrobial activity based on the bacterial time-kill assay using S. aureus, but the efficiency was affected by both nHA precipitation and drug loading strategy. Highest antimicrobial activity was seen in the samples without nHA layer, and when the drug was loaded by diffusion. Fourier transform infrared spectroscopy and X-ray diffraction analyses revealed that nHA on the surface enhanced HA growth in simulated body fluid for 3 weeks, showing increased osseointegration potential. Therefore, the proposed coating may be used to prevent Ti implant failure originated from bacterial infection and/or low bioactivity.

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Behavior of mammalian cells on magnesium substituted bare and hydroxyapatite deposited (Ti,Mg)N coatings

Cited by 13 publications

References 51 publications

Assessment of bone healing using (Ti,Mg)N thin film coated plates and screws: Rabbit femur model

Assessment of bone healing using (Ti,Mg)N thin film coated plates and screws: Rabbit femur model

Biocompatibility and Immune Response of a Newly Developed Volume-Stable Magnesium-Based Barrier Membrane in Combination with a PVD Coating for Guided Bone Regeneration (GBR)

A functional coating to enhance antibacterial and bioactivity properties of titanium implants and its performance in vitro

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