Behavior of DNA Markers Linked to a Clubroot Resistance Gene in Segregating Populations of Chinese Cabbage (Brassica rapa ssp. pekinensis)

Matsumoto, Etsuo; Hayashida, Nobuaki; Sakamoto, Koji; Ohi, Michio

doi:10.2503/jjshs.74.367

Cited by 27 publications

(38 citation statements)

References 21 publications

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“…T136-8 and K13 possess a resistance locus CRa (designated here as resistance gene A) (Matsumoto et al, 1998). K10 possesses CRk (gene B), and C9 and RC22 possess CRc (gene C) .…”

Section: Plant Materials and Breeding Proceduresmentioning

confidence: 99%

“…Amplification was performed as follows: 94°C for 3 min, 30 cycles of 94°C for 1 min, 55°C for 1.5 min, and 72°C for 2 min, followed by a final extension of 7 min at 72°C. Electrophoresis of amplified products was completed and band patterns were observed as previously described (Matsumoto et al, 2005).…”

Section: Marker Selectionmentioning

confidence: 99%

“…DNA markers for clubroot resistance have been developed in major Brassica crops. Seven major CR loci, CRa, CRb, CRk, CRc, Crr1, Crr2, and Crr3, have been identified for Chinese cabbage using various types of markers (Hirai et al, 2004;Matsumoto et al, 1998;Piao et al, 2004;Sakamoto et al, 2008;Suwabe et al, 2003), though mutual relations among them are somewhat ambiguous, because different resistant materials and pathogenic isolates are employed for detecting the loci. The complexity of the clubroot pathogen in particular makes an exact evaluation of resistance genes difficult.…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of Three Clubroot Resistance Genes through Marker-assisted Selection in Chinese Cabbage (Brassica rapa ssp. pekinensis).

Matsumoto¹,

Ueno

Aruga

et al. 2012

J. Japan. Soc. Hort. Sci.

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Many clubroot resistant (CR) cultivars of Chinese cabbage (Brassica rapa ssp. pekinensis) have been bred so far, but their usage is limited because the capacity for resistance breaks down with time. This degradation is caused by a pathogenic variation in the causal fungus Plasmodiophora brassicae. We attempted to accumulate 3 CR genes, CRa, CRk, and CRc, through marker-assisted selection. Five doubled haploid CR lines with an individual CR locus were used as breeding materials. The CR lines were crossed with each other. A subsequent selection for resistance was performed using sequence characterized amplified region markers in segregating generations. As a result, 4 homozygous lines for 3 resistance genes and the F 1 hybrids between them were developed. CR pyramiding lines were inoculated with 6 field isolates of P. brassicae. The homozygous lines for 3 CR genes, whether selfed or crossed, exhibited exceedingly high resistance against all of the isolates. Morphological characters of F 1 hybrids were comparable to those of a control cultivar, but the degree of heterosis was less than expected, which is probably because of genetic similarity of the parents. The results of this study prove that clubroot resistance can be reinforced through the accumulation of varied resistance genes in B. rapa.

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“…T136-8 and K13 possess a resistance locus CRa (designated here as resistance gene A) (Matsumoto et al, 1998). K10 possesses CRk (gene B), and C9 and RC22 possess CRc (gene C) .…”

Section: Plant Materials and Breeding Proceduresmentioning

confidence: 99%

Section: Marker Selectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Accumulation of Three Clubroot Resistance Genes through Marker-assisted Selection in Chinese Cabbage (Brassica rapa ssp. pekinensis).

Matsumoto¹,

Ueno

Aruga

et al. 2012

J. Japan. Soc. Hort. Sci.

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“…CR in turnips is considered to be qualitative and is grouped into several genotypical categories corresponding to the pathotype of P. brassicae (Matsumoto et al, 2005). It is important to monitor the inheritance of these resistant genes using molecular markers in the breeding of Chinese cabbage (Kuginuki et al, 1997;Matsumoto et al, 2005;Suwabe et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Several breeding programs have been successfully carried out to develop clubroot resistance (CR) cultivars of Chinese cabbage using European turnips (B. rapa ssp. rapifera) as sources of resistance (Hirai et al, 2004;Matsumoto et al, 1998). CR in turnips is considered to be qualitative and is grouped into several genotypical categories corresponding to the pathotype of P. brassicae (Matsumoto et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Construction of a Practical SCAR Marker Linked to Clubroot Resistance in Chinese Cabbage, with Intensive Analysis of HC352b Genes

Hayashida

Takabatake

Nakazawa

et al. 2008

J. Japan. Soc. Hort. Sci.

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A restriction fragment length polymorphism (RFLP) marker, HC352b, has identified as closely linked to the CRa locus responsible for clubroot resistance (CR) in a study dissecting CR loci in Chinese cabbage (Brassica rapa L. ssp pekinensis). Unfortunately, the RFLP pattern, including HC352b detected by the cDNA clone HC352, was complicated and confused in its interpretation because it represented multiple-copy loci. To provide a practical HC352b marker for CR breeding programs, a sequence characterized amplified region (SCAR) marker was constructed along the analysis of HC352 genes, followed by its evaluation for CR selection. Schematic characterization of signals was achieved by identification of HC352 homologous genes with their restriction sites, employing CRa-positive and -negative doubled haploid (DH) lines. Genomic sequence information from a set of HC352 homologous genes was analyzed to identify the CRa linked paralog HC352b, followed by the successful designation of a SCAR marker. This correctly predicted the CR phenotypes of all tested individuals of F 2 and back-crossed progenies.

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Identification of a genomic region containing genes involved in resistance to four pathotypes of Plasmodiophora brassicae in Brassica rapa turnip ECD02

Rahaman

Strelkov

et al. 2022

The Plant Genome

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Clubroot, caused by Plasmodiophora brassicae, is an important disease of brassica crops worldwide. Vegetable turnip (Brassica rapa L.) have proven to be a source of clubroot resistance genes effective against many pathotypes of P. brassicae. The F 1 progeny from the cross B. rapa canola ACDC (susceptible, S) × B. rapa turnip ECD02 (resistant, R) were backcrossed with ACDC, then self-pollinated to produce BC 1 S 1 lines. All the F 1 plants were resistant to four pathotypes (3A, 3D, 3H, and 5X) of P. brassicae. Segregation for R and S in BC 1 to each pathotype was 1:1 and resistance reactions were highly correlated. From whole genome sequencing, 192.1 M sequences with 96% template coverage from ECD02, and 478.9 M sequences with 92% coverage from ACDC, were aligned with the reference genome of B. rapa.Genotyping-by-sequencing was performed on the BC 1 population. The number of aligned short reads per plant in the BC 1 ranged from 1.4 to 8.5 M sequences with 4-8% template coverage. We obtained 1,344 high-quality single-nucleotide polymorphism (SNP) loci with a mean missing rate at 0.27% and distributed them on 10 chromosomes. A single co-localized quantitative trait loci (QTL), designated as Rcr9 ECD02 on chromosome A08, conferred resistance to the four pathotypes. The QTL explained 68.9-74.4% of phenotypic variation with the logarithm of the odds values of 24.3 to 31.1. Bulked segregant analysis was performed, and 14 SNP markers linked to the gene were developed using the Kompetitive Allele Specific PCR.Rcr9 ECD02 was mapped into an interval of 2.2 cM, flanked by CF_A08_10664692 and CF_A08_12230973, which spanned 1.51 Mb on the chromosome and

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Behavior of DNA Markers Linked to a Clubroot Resistance Gene in Segregating Populations of Chinese Cabbage (Brassica rapa ssp. pekinensis)

Cited by 27 publications

References 21 publications

Accumulation of Three Clubroot Resistance Genes through Marker-assisted Selection in Chinese Cabbage (Brassica rapa ssp. pekinensis).

Accumulation of Three Clubroot Resistance Genes through Marker-assisted Selection in Chinese Cabbage (Brassica rapa ssp. pekinensis).

Construction of a Practical SCAR Marker Linked to Clubroot Resistance in Chinese Cabbage, with Intensive Analysis of HC352b Genes

Identification of a genomic region containing genes involved in resistance to four pathotypes of Plasmodiophora brassicae in Brassica rapa turnip ECD02

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