Behavior of Different Suture Materials in the Urinary Bladder of the Rabbit with Special Reference to Wound Healing, Epithelization and Crystallization

Abstract

Five urinary bladder incisions were performed in 16 rabbits and the defects closed by plain catgut 4-0, chromic catgut 4-0, polypropylene 4-0, polyglactin 910 4-0 and polyglactin 910 8-0 in the form of an all-layer suture, thereby obtaining a total of 80 suture sites. The running sutures in 8 animals were reviewed by light microscopy after 1, 2, 7 and 15 weeks, and in 8 additional animals after 3 days, 1,2,7 and 15 weeks by scanning electron microscopy. Moreover, we analyzed and compared the autopsy findings on gross inspection, the urinary sediments and urinary cultures of the individual animals. Scanning electron-microscopic evaluations showed that epithelization of the intraluminal suture portions can be accomplished after 3 days already. Reduced suture strength and rapid absorption of the suture material prevent later postoperative incrustations. Light-microscopic inspection of the segments of the bladder wall bearing this suture material suggests that a minor inflammatory tissue response around the suture material – with polyglactin 910 8-0 in particular – is more likely to enhance regeneration of the tunica muscularis and to prevent formation of cysts than seen with a long-standing highly inflammatory tissue response around the suture site of catgut threads. In the animal experiment, the polyglactin 910 suture has shown sufficient firmness and proved to be superior to any other suture material as regards the reaction to foreign bodies and inclination to incrustation. A thinner thread used for sutures in the efferent urinary tract would thus have to be given preference over others. Plain catgut, chromic catgut and polyglactin 910 sutures, 4-0 in strength, were found to be disadvantageous in the animal model. The nonabsorbable propylene thread turned out to be inadequate in urinary bladder surgery.

Tissue Reaction to Absorbable Endoloop, Nonabsorbable Titanium Staples, and Polymer Hem-o-lok Clip After Laparoscopic Appendectomy

“…Foreign body reaction was correlated with the duration of suture absorption, developing at the end of the absorption processes. 12 Thus, one way that we can explain the lower level of foreign body reaction to these materials is that both Hem-o-lok clips and titanium stapler line are nonabsorbable materials.…”

Molecular Switch Controlling the Binding of Anionic Bile Acid Conjugates to Human Apical Sodium-Dependent Bile Acid Transporter

“…Surface area terms were obtained as averages over the conformations saved from the MD simulations. Molecular weight (MW), molar refractivity (MR), number of rotatable bonds (brotN) and flexibility parameter (KierFlex) were calculated using the program MOE35. Additionally, number of hydrogen bond donors (HD) and hydrogen bond acceptors (HA) for each molecule was calculated using Discovery Studio (Accelrys Inc.) 36…”

Structural Determinants for Transport across the Intestinal Bile Acid Transporter Using C-24 Bile Acid Conjugates

“…Surface area terms were obtained as averages over the conformations saved from the MD simulations. Molar refractivity (MR) and flexibility parameter (KierFlex) were calculated using the program MOE 35. The number of hydrogen bond donors (HD) for each molecule was calculated using Discovery Studio (Accelrys Inc.) 36.…”