Behavior of cultured cells on substrata of variable adhesiveness

Harris, Albert K.

doi:10.1016/0014-4827(73)90579-x

Cited by 262 publications

(77 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The nonspecific characteristics of chemotactic deactivation (cross-deactivation and decreased translocation in the absence of a chemotactic gradient) may therefore be causally linked to the sustained enhancement of adherence. An association of increased resistance to detachment and decreased migration has been observed in certain tissue cells (5,6) and in macrophages exposed to lymphokines (31).…”

Section: Discussionmentioning

confidence: 96%

“…Attachment to a surface seems to be a prerequisite for migration in vitro (1) (e.g., to glass or plastic) and in vivo (2) (e.g., to endothelial cells), and has been thought to contribute to the development of the polarized cellular shape assumed by neutrophils during migration in vitro (3). There is considerable evidence to support the idea that the nature of the interaction of some cell types with the substratum in vitro influences translocation (1,(4)(5)(6). The influence may be on the direction of movement (e.g., haptotaxis) or the rate of movement.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Motility and Adhesiveness in Human Neutrophils

Smith¹,

Hollers²,

Patrick³

et al. 1979

J. Clin. Invest.

220

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 99%

Motility and Adhesiveness in Human Neutrophils

Smith¹,

Hollers²,

Patrick³

et al. 1979

J. Clin. Invest.

220

View full text Add to dashboard Cite

“…HUVE cells that grew to confluency on EDA and EDA/hFN substrates were cultured in growth medium supplemented with bFGF at 5 ng/ml without endothelial cell growth factor, as suggested by Maciag et al (19). After an additional [10][11][12][13][14][15][16][17][18][19][20][21] days in culture, HUVE cells underwent assembly into preneovascular structures, forming braids of cells and complex multicellular strands (Fig. 2).…”

Section: Methodsmentioning

confidence: 99%

Spatially controlled adhesion, spreading, and differentiation of endothelial cells on self-assembled molecular monolayers.

Spargo

Testoff

Nielsen

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

116

View full text Add to dashboard Cite

Chenaically moid glass substrates were used to denste dirental adheso, growth, and differention f endoel cells. Endothellal cells were eamine for Madei and growth on glass, glass treated with N-(2-aminethyl)-3-amnopropyl trimethoxysilane (EDA) Several approaches have also demonstrated the potential for controlling the spatial distribution of cells on the surface. Carter (8, 9) and later Harris (10), using acetate-coated glass, created palladium islands and channels for studying single fibroblast cell reactions and clone formation. Kleinfeld and coworkers (11) fabricated high-resolution patterns of selfassembled monolayers (SAMs) of amino-and perfluoroalkylsilanes, which resulted in dissociated neurons that preferentially adhered to and developed electrically active associations within the geometrically defined regions. The use of SAMs to create regions of defined chemical reactivity also allows selective attachment of fibroblasts and endothelial cells to regions modified by covalently bound peptides that contain the Arg-Gly-Asp (RGD) binding domain (12)(13)(14).In this study, a series of SAM-modified surfaces were examined for their ability to support adherence and facilitate growth and differentiation of endothelial cells. Patterned substrates, created by deep UV photolithography (15), were used to provide spatially defined regions that promoted adhesion, spreading ofendothelial cells, and ultimately spatially defined neovascular assemblies on a two-dimensional surface. MATERIALS AND METHODSReagents. Human fibronectin (hFN) was obtained from Collaborative Research. Heparin sulfate (HS) (47.5 kDa) was obtained from Sigma. N-(2-Aminoethyl)-3-aminopropyl trimethoxysilane (EDA) and (tridecafluoro-1,1,2,2-tetrahydrooctyl)-l-dimethylchlorosilane (13F) were used as procured from Hflls America (Bristol, PA).Substrate Preparation. Patterned deposition of EDA and 13F was accomplished by using the photolithography method of Dulcey et al. (15). Glass substrates were washed in methanol/HCl, 1:1 (vol/vol) for 1 hr and 18 M H2SO4 for 1 hr followed by multiple washes with deionized water (18 Mfl). After the final wash, the substrates were immersed in boiling deionized water for 20 min, removed, and immersed in 1% EDA/94% (vol/vol) methanol/i mM acetic acid/5% H20 for 15 min. The substrates were then rinsed at least three times in anhydrous methanol and baked at 1200C for 5 min.13F was deposited on either clean glass or patterned EDA surfaces by using a 1% (vol/vol) solution of the silane in anhydrous toluene for 1-3 hr, followed by at least four rinses in toluene, and baked 5 min at 120'C. EDA films were exposed to pulsed ArF excimer laser (193 nm) radiation (15 J/cm2, 30 Hz) through a lithographic mask. Deep UVirradiated regions of the substrate were resilanized with 13FAbbreviations: SAM, self-assembled monolayer; HUVE, human umbilical vein endothelial cell; EDA, N-(2-aminoethyl)-3-aminopropyl trimethoxysilane; 13F, (tridecafluoro-1,1,2,2-tetrahydrooctyl)-1-dimethylchlorosilane; bFGF, basic fibroblast growth factor; ...

show abstract

“…A second set of substrates was prepared with albumin as described above but, in addition, we placed a drop of antialbumin rabbit serum on top of the albumin for 1 min and then rinsed and dried the slide as before. At this spot the slide now had a double layer of protein (2) Harris (9) that, given a choice between two surfaces, the cells prefer the more hydrophilic surface. This preference is surprising because the substrates are covered with the same protein molecules.…”

Section: Methodsmentioning

confidence: 99%

Cell adhesion to substrates containing adsorbed or attached IgG.

Giæver¹,

Ward²

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

When most mammalian cells are propagated in tissue culture, they are attached to a solid surface. This surface is commonly covered with a layer of serum protein. In this study we looked at the effect of the protein layer on the interaction between the cells and the surface by precoating the surface with various pure protein molecules. We found no differences between surfaces covered with pure protein layers and those covered with serum proteins except when antibody molecules were used. In all cases the cell types used avoided surfaces covered with antibody molecules. Previously, other investigators have established that some cells belonging to the immunology system are attracted to such surfaces. The nature of the interactions of a cell with other cells and with a substrate is an important problem in biology. One method of studying these interactions is to grow cells in tissue culture. When mammalian cells are grown in vitro, most cells do not divide unless they are attached to a solid surface. Under normal conditions, cells growing in the presence of serum (a few percent) never contact a bare surface because, at these protein concentrations, the surfaces will be covered with a monolayer of serum proteins (1, 2) before cell attachment. Witkowski and Brighton (3) recognized that this protein layer probably is important and that it modifies the substrate properties. Grinnel (4) also recognized the importance of the protein layer. In particular, he isolated a serum protein that he believes to be a cell-spreading factor (5).In this note we report on our observations that some primary cell cultures do not grow well on antibodies (IgG) adsorbed on a surface and that they avoid surfaces covered with antigenantibody complexes. It is interesting to compare this observation to some recent results of Alexander and Henkart (6). They found that a subclass of human lymphocytes spreads on top of antibody-antigen complexes, even though, in contrast to other cells, lymphocytes rarely adhere to surfaces in vitro. Because the histocompatibility antigens are known to be closely related to the antibodies (7), it is interesting to speculate on their importance in cell-cell recognition. MATERIALS AND METHODSSeveral different substrates were used: glass, Lexan, gold films evaporated onto Lexan, and indium particles evaporated onto glass The last substrate has the advantage that single and double layers of protein are both distinguishable and visible to the unaided eye (2). The substrates measured-about 25 X 25 mm. Approximately half of the substrate was dipped into a protein solution (1 mg/ml) for 1 min, rinsed in running distilled water for a few seconds, and blown dry with compressed air. Thus, when a substrate was used, one half contained a monolayer of protein from the dipping solution and the other half contained a monolayer adsorbed from the serum added to the culture medium. In this manner we used, as a dipping solution, horse serum, calf serum, trypsin, bovine serum albumin, and bovine IgG. A second set of substrates ...

show abstract

Behavior of cultured cells on substrata of variable adhesiveness

Cited by 262 publications

References 12 publications

Motility and Adhesiveness in Human Neutrophils

Motility and Adhesiveness in Human Neutrophils

Spatially controlled adhesion, spreading, and differentiation of endothelial cells on self-assembled molecular monolayers.

Cell adhesion to substrates containing adsorbed or attached IgG.

Contact Info

Product

Resources

About