Begomovirus Detection On Diseased Chili Plant (Capsicum annum L.) In Tanah Karo North Sumatera With PCR Techniques

Hannum, Saleha; Aceh, Rahmi Maulidiyah; Elimasni,

doi:10.1088/1755-1315/305/1/012057

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…Serangan PepYLCV pada cabai telah dilaporkan di Jawa Tengah, Yogyakarta, Jawa Barat, Sumatra Utara, dan Sumatra Barat (Sulandari et al 2006;Trisno et al 2009;Hannum et al 2019). Pada tahun 2003 serangan penyakit ini menyebabkan gagal panen dan pemusnahan cabai pada beberapa daerah sentra produksi, seperti Yogyakarta dan Lampung (Sulandari et al 2006).…”

Section: Pendahuluanunclassified

Resistance Analysis of CRISPR/Cas9 Genome-Edited Chili M2 Mutant Lines against Pepper Yellow Leaf Curl Viral Disease

Prasetya

Hadiarto

Enggarini³

et al. 2021

J. AgroBiogen

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<p>Pepper yellow leaf curl virus (PepYLCV) infection transmitted by silverleaf whitefly (Bemisia tabaci [Gennadius]) can decrease chili pepper yield up to 100%. At this moment, there is no chili pepper variety resistant to PepYLCV available. Genome editing approach through CRISPR/Cas9 is an effort to develop variety resistance to the viral infection. The purpose of this study was to obtain M2 lines developed by CRISPR/Cas9 system on proliferating cell nuclear antigen (PCNA) gene for resistance to PepYLCV. A total of four M2 lines (C47-7, L84-2, L84-23, and L120-19) consisting of 60 chili plants were tested for their resistance to PepYLCV. PCR analysis was performed to detect the presence (infection) of the virus. The results showed that a total of 35 plants derived from the four lines were resistant to PepYLCV. They consisted of 7 plants from C47-7 line, 11 plants from L84-2 line, 9 plants from L84-23 line, and 8 plants from L120-19 line. PCR analysis confirmed that the resistant plants obtained from this study were negatively infected by the virus. Since not all tested plants were resistant to virus infection, the PCNA gene allele in these resistant lines were most likely heterozigotes. Sequencing of PCNA gene of the resistant lines is needed to confirm that the resistance phenotypes obtained was due to mutation of the gene. Therefore, further selection needs to be performed to obtain stable and PepYLCV-resistant lines.</p>

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Section: Pendahuluanunclassified

Resistance Analysis of CRISPR/Cas9 Genome-Edited Chili M2 Mutant Lines against Pepper Yellow Leaf Curl Viral Disease

Prasetya

Hadiarto

Enggarini³

et al. 2021

J. AgroBiogen

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“…Upward leaf curling and mosaic are found in pepper plants and found in other plants such as tomato, which is infected by TYLCV (Tomato Yellow Leaf Curl Virus), and bean, which is infected by BCMV (Bean Common Mosaic Virus). Different soil fertility levels, climate, plant age, and plant genotype have also contributed to these different symptoms (Hannum, et al, 2019). Table 6 described that the incubation period and disease intensity varied.…”

Section: Plant Resistance Against Begomovirusmentioning

confidence: 99%

Estimation of Genetic Parameters of Cayenne Peppers (Capsicum Annuum L.) from IPB University for Its Resistance Against Begomoviruses in Aceh

et al. 2020

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The productivity of pepper plants is strongly influenced by climatic and weather conditions as well as pests and diseases. One of the major losses in chili farming is the pest attack, such as whiteflies (Bemisia tabaci Genn.), a virus vector. Evaluation of the resistance of pepper plant germplasm against Begomovirus infection needs to be carried out, evaluate genetic diversity, estimate heritability values, and obtain a selection character for the resistance of chili plants Begomovirus. This research was conducted at Experimental Garden, Faculty of Agriculture, Universitas Syiah Kuala, and the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Universitas Syiah Kuala from January to May 2020. Three varieties Gada, Anies and Selekta with seven test genotypes i.e F6 Gada, Seloka 4-10-2-2, F5136074-1-4-3, F5074136-2-3-2, F5074035-2-1-2, F5074035-2-4-2, F5074077-1-1-3 were screened for their resistance against Begomovirus. This study was designed using a non-factorial Randomized Block Design (RBD) and scoring parameter Begomovirus symptom. Tukey’s HSD, the standard deviation of genetic variance, heritability, and correlation were performed to analyze the data. The lowest disease intensity occurred in genotype F6 Gada with a percentage of 12.08% disease intensity (slightly resistant), and the highest level of disease intensity occurred in F5074035-2-4-2 with a percentage of 30.94% (susceptible). Variables symptom scoring, disease intensity, and incubation period were the most effective variables for selecting pepper resistance against Begomovirus infection based on broad-sense heritability values.

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“…The Begomovirus genus that infects pepper plants can cause a decrease in yield loss by up to 100% [ 12 ]. In the field, symptoms of begomovirus infection on pepper are very typical such as chlorosis, mosaic, vein clearing, and bleaching [ 13 ]. Viral infections such as yellow curly leaves on pepper plants have spread rapidly into central pepper plants in Yogyakarta, Bali, and Nusa Penida [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Current Status and Complexity of Three Begomovirus Species in Pepper Plants in Lowlands and Highlands in Java Island, Indonesia

Wahyono

Murti

Hartono

et al. 2023

Viruses

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Three primary species from the Begomovirus genus, Pepper yellow leaf curl Indonesia virus (PepYLCIV), Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), and Tomato leaf curl New Delhi virus (ToLCNDV), are suspected of spreading throughout pepper production centers, and plants are infected by a single species or a combination of two or three species. This study was conducted to provide complete information about the symptoms, incidence and severity, whitefly biotypes, as well as the dominance status of the three Begomovirus species in pepper-producing areas in Java. A DNA analysis was carried out on leaf samples to identify Begomovirus species and biotypes of B. tabaci collected from 18 areas (16 districts) in lowlands (<400 m asl) and highlands (>700 m asl). The DNA analysis showed that B. tabaci biotype B was the most commonly detected in all locations compared to the A, AN, and Q biotypes. The incidence of begomovirus infection was at a high level, 93% and 88.78% in the lowlands and highlands, respectively. However, the severity of begomovirus infection was significantly higher in the lowlands (54.50%) than in the highlands (38.11%). A single infection of PepYLCIV was most dominant in all locations sampled and caused severe infection, followed by a mixed infection with TYLCKaV. Therefore, the current status of begomovirus infection, especially PepYLCIV, can provide advice to farmers using more tolerant and resistant varieties as well as a breeding strategy for resistant pepper varieties.

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Begomovirus Detection On Diseased Chili Plant (Capsicum annum L.) In Tanah Karo North Sumatera With PCR Techniques

Cited by 5 publications

References 3 publications

Resistance Analysis of CRISPR/Cas9 Genome-Edited Chili M2 Mutant Lines against Pepper Yellow Leaf Curl Viral Disease

Resistance Analysis of CRISPR/Cas9 Genome-Edited Chili M2 Mutant Lines against Pepper Yellow Leaf Curl Viral Disease

Estimation of Genetic Parameters of Cayenne Peppers (Capsicum Annuum L.) from IPB University for Its Resistance Against Begomoviruses in Aceh

Current Status and Complexity of Three Begomovirus Species in Pepper Plants in Lowlands and Highlands in Java Island, Indonesia

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