Befruchtungs- und Entwicklungskompetenz nonovulatorischer Katzenoozyten nach Kokultivierung mit epididymalen Spermien und felinen Oviduktepithelzellen

Lengwinat, T.; Pitra, C.; Blotmer, S.

doi:10.1111/j.1439-0531.1992.tb01141.x

Cited by 5 publications

(2 citation statements)

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“…As in vivo experiments are technically challenging and ethically questionable, in vitro models are established in accordance to the 3R principle (replacement, refinement, reduction of animal experiments). So far, mainly suspension cultures (Lengwinat et al 1992 ; Eder et al 2020 ) or in one case, 2D adherent submerged cultures (Roth et al 1993 ) have been used for studying feline oviduct epithelium physiology. The two main applications of feline oviduct epithelial cell (FOEC) cultures were the characterization of sperm–oviduct interactions (Henry et al 2015 ; Eder et al 2020 ) and the improvement of fertilization and developmental competence of early cat embryos (Lengwinat et al 1992 ; Roth et al 1993 ; Swanson et al 1996 ).…”

Section: Introductionmentioning

confidence: 99%

“…So far, mainly suspension cultures (Lengwinat et al 1992 ; Eder et al 2020 ) or in one case, 2D adherent submerged cultures (Roth et al 1993 ) have been used for studying feline oviduct epithelium physiology. The two main applications of feline oviduct epithelial cell (FOEC) cultures were the characterization of sperm–oviduct interactions (Henry et al 2015 ; Eder et al 2020 ) and the improvement of fertilization and developmental competence of early cat embryos (Lengwinat et al 1992 ; Roth et al 1993 ; Swanson et al 1996 ). However, studies on bovine and porcine oviduct epithelial cells have shown that 2D adherent submerged and suspension culture conditions rapidly lead to changes in cell type-specific morphology and functionality, including loss of polarization, impaired barrier formation, and altered gene expression (Reischl et al 1999 ; Rottmayer et al 2006 ; Danesh Mesgaran et al 2016 ; Chen and Schoen 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Long-term culture of feline oviduct epithelial cells on permeable filter supports

et al. 2022

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Basic knowledge about cellular and molecular mechanisms underlying feline reproduction is required to improve reproductive biotechnologies in endangered felids. Commonly, the domestic cat (Felis catus) is used as a model species, but many of the fine-tuned, dynamic reproductive processes can hardly be observed in vivo. This necessitates the development of in vitro models. The oviduct is a central reproductive organ hosting fertilization in the ampulla and early embryonic development in the isthmus part, which also functions as a sperm reservoir before fertilization. In other species, culturing oviduct epithelial cells in compartmentalized culture systems has proven useful to maintain oviduct epithelium polarization and functionality. Therefore, we made the first attempt to establish a compartmentalized long-term culture system of feline oviduct epithelial cells from both ampulla and isthmus. Cells were isolated from tissue samples (n = 33 animals) after routine gonadectomy, seeded on permeable filter supports and cultured at the liquid–liquid or air–liquid interface. Cultures were harvested after 21 days and microscopically evaluated for epithelial differentiation (monolayer formation with basal–apical polarization) and protein expression of marker genes (oviduct-specific glycoprotein, acetylated tubulin). Due to the heterogeneous and undefined native tissue material available for this study, the applied cell culture approach was only successful in a limited number of cases (five differentiated cultures). Even though the protocol needs optimization, our study showed that the compartmentalized culture approach is suitable for maintaining differentiated epithelial cells from both isthmus and ampulla of the feline oviduct.

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