Before Maternal-Zygotic Transition … There Was Morphogenetic Function of Nuclei

Korzh, Vladimir

doi:10.1089/zeb.2008.0573

Cited by 16 publications

(9 citation statements)

References 41 publications

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“…All of the enriched categories are listed (Figure 5B). Interestingly, consistent with the fact that maternally stored mRNAs direct early embryonic development prior to MBT (Korzh, 2009; Yasuda and Schubiger, 1992), genes with oocyte-specific hypomethylated promoters (oocyte versus sperm and oocyte versus MBT) show strong enrichment in the processes that regulate very early embryogenesis such as cellular component morphogenesis, cell motion, and lateral line system development (Figure 5B, orange window).…”

Section: Resultssupporting

confidence: 61%

Sperm, but Not Oocyte, DNA Methylome Is Inherited by Zebrafish Early Embryos

Jiang

Zhang

Wang

et al. 2013

Cell

437

400

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SUMMARY 5-methylcytosine is a major epigenetic modification that is sometimes called “the fifth nucleotide.” However, our knowledge of how offspring inherit the DNA methylome from parents is limited. We generated nine single-base resolution DNA methylomes, including zebrafish gametes and early embryos. The oocyte methylome is significantly hypomethylated compared to sperm. Strikingly, the paternal DNA methylation pattern is maintained throughout early embryogenesis. The maternal DNA methylation pattern is maintained until the 16-cell stage. Then, the oocyte methylome is gradually discarded through cell division and is progressively reprogrammed to a pattern similar to that of the sperm methylome. The passive demethylation rate and the de novo methylation rate are similar in the maternal DNA. By the midblastula stage, the embryo’s methylome is virtually identical to the sperm methylome. Moreover, inheritance of the sperm methylome facilitates the epigenetic regulation of embryogenesis. Therefore, besides DNA sequences, sperm DNA methylome is also inherited in zebrafish early embryos.

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Section: Resultssupporting

confidence: 61%

Sperm, but Not Oocyte, DNA Methylome Is Inherited by Zebrafish Early Embryos

Jiang

Zhang

Wang

et al. 2013

Cell

437

400

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“…The higher expression level of lineage-specific genes after the MBT suggests that lineage-specific genes are important components for the zygotic transcription. Maternally deposited mRNAs direct early development before the initiation of zygotic transcription during mid-blastula transition [95]. However, zygotic transcription plays a more important role in the regulation of development after MBT, since a high percentage of maternally stored mRNA has been degraded during the post-MBT stages.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, characterization, and expression analysis of lineage-specific genes within zebrafish

Yang

Zou

et al. 2013

BMC Genomics

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BackgroundThe genomic basis of teleost phenotypic complexity remains obscure, despite increasing availability of genome and transcriptome sequence data. Fish-specific genome duplication cannot provide sufficient explanation for the morphological complexity of teleosts, considering the relatively large number of extinct basal ray-finned fishes.ResultsIn this study, we performed comparative genomic analysis to discover the Conserved Teleost-Specific Genes (CTSGs) and orphan genes within zebrafish and found that these two sets of lineage-specific genes may have played important roles during zebrafish embryogenesis. Lineage-specific genes within zebrafish share many of the characteristics of their counterparts in other species: shorter length, fewer exon numbers, higher GC content, and fewer of them have transcript support. Chromosomal location analysis indicated that neither the CTSGs nor the orphan genes were distributed evenly in the chromosomes of zebrafish. The significant enrichment of immunity proteins in CTSGs annotated by gene ontology (GO) or predicted ab initio may imply that defense against pathogens may be an important reason for the diversification of teleosts. The evolutionary origin of the lineage-specific genes was determined and a very high percentage of lineage-specific genes were generated via gene duplications. The temporal and spatial expression profile of lineage-specific genes obtained by expressed sequence tags (EST) and RNA-seq data revealed two novel properties: in addition to being highly tissue-preferred expression, lineage-specific genes are also highly temporally restricted, namely they are expressed in narrower time windows than evolutionarily conserved genes and are specifically enriched in later-stage embryos and early larval stages.ConclusionsOur study provides the first systematic identification of two different sets of lineage-specific genes within zebrafish and provides valuable information leading towards a better understanding of the molecular mechanisms of the genomic basis of teleost phenotypic complexity for future studies.

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“…A previous study on the zygotic transition of zebrafish revealed that clusters of genes were characterized by very low expression during pre-MBT stages and showed high abundance from 3.5 hpf or 5.3 hpf, which were referred to as zygotic supercluster, demonstrating the existence of a mechanism driving development prior to ZGA (Neyfakh 1956;Newport 1982;Korzh 2009). In the present study, we found the level of expression of retrocopies increased from the 1000-cell stage, which is close to the MBT, peaking at the shield stage, close to the post-MBT stage, representing a similar expression pattern to the zygotic supercluster genes before budding (Fig.…”

Section: Temporal and Spatial Expression Patterns Of Retrocopiesmentioning

confidence: 98%