Beeinflussung von Virulenz und Immunogenität beiPasteurella multocida durch Eisenin vitro

Kd, Flossmann; Rosner, H.; U, Grünke; Miosga, N.

doi:10.1002/jobm.3630240404

Cited by 9 publications

(1 citation statement)

References 22 publications

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“…Similar growth observed on tryptic soy agar (light-white colonies of the same size), bile-chrysoidinglycerol agar according to Ziesche! et al (1985) (greenblue colonies of about 1 mm in diameter), King A agar (no pigmentation and no fluorescence under UV 365 nm), Tween 80 agar, gelatinase agar, starch agar, CDM-H agar (a chemically defined medium ; Flossmann et al, 1984). Good growth in nutrient broth and in tryptic soy broth at 37 mC for 24 h. No growth on…”

Section: Discussionmentioning

confidence: 99%

Brackiella oedipodis gen. nov., sp. nov., gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus).

Willems¹,

Gilhaus²,

Beer³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Section: Discussionmentioning

confidence: 99%

Brackiella oedipodis gen. nov., sp. nov., gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus).

Willems¹,

Gilhaus²,

Beer³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Untersuchungen zur Oberfläuchenstruktur von Pasteurella multocida

Rosner

Rohrmann

Günther

1990

Acta Biotechnologica

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Akademie der Landwirtschaftswissenschaf ten der DDR Institut fur bakterielle Tierseuchenforschung, Jena Naumburger StraSe 96a, Jena 6909, DDR Vortrag anlaBlich des 4. Leipziger Biotechnologie-Symposiums 12. -16. Dezember 1988 Summary Detailed studies of the surface structures are an important requirement for the development of efficient vaccines against enzootic pneumonia in calves and piglets caused by Pasteurella multocida. Electron microscopical examination after Alcian Blue staining shows capsular material extending far into the surrounding medium. The extent of the capsules depicted by light microscopy did not correlate with virulence and immunogenicity. However, the extent of the capsules was effected by different culture conditions. The immunization with extract material resulted in a good protection against homologous infection. It has been shown that different cultivation conditions can result in heterogeneity of LPS and in altered OMP-profiles.Bei der Auseinandersetzung des Bakteriums mit seiner Umwelt wahrend des Infektionsverlaufes kommt der bakteriellen Oberflache eine besondere Bedeutung zu. Sie ist verantwortlich fur wichtige Funktionen wie Bindungsort von antigenem Material, Adhasion a n Zelloberflachen oder Schutz des Baktkriums vor Umwelteinflussen. Umfassende Kenntnisse uber das an der Oberflache gebundene Material sind in entscheitlendem MaSe fur die Entwicklung effektiver Impfstoffe notwendig. I n der vorliegenden Untersuchung interessierte uns der EinfluB unterschiedlicher Kultivierungsbedingungen auf dieses Material sowie auf die Veranderung biologischer Parameter wie Virulenz und Immunogenitat. Material und MethodenBakterien und Kultivierungsbedingungen F u r die Untersuchungen wurden die P. multocida -Stilmme 880 (Serotyp A nach CARTER) und 383 (Serotyp B nach CARTER) eingesetzt. Die Isolierung beider Spezies erfolgte aus Kalbern im Institut fur bakterielle Tierseuchenforschung, Jena. Die Kultivierung ging von der Stammhaltung auf Blutagarplatten &us, gefolgt von dreimaliger Passagierung durch Mause. Die weitere Passagierung in chemisch definierten Fllissigmedien nach FLOSSMANN e t al. [l] (Medium 906: Eisen-Normalmedium; Medium 902: Eisen-Mangelmedium)

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Nachweis von Eisen‐Transport‐Aktivität in Pasteurella multocida‐Kulturen

Flossmann¹,

Grajetzki²,

Rosner³

1985

J. Basic Microbiol.

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It has been established that Pasteurella multocida cultures possess pronounced iron transport activities to accumulate the iron necessary for growth. Experiments with Fe-59 confirmed that the bacterial cells are able to acquire iron without direct contact from high molecular iron substrates, such as iron dextrane, ferritine or transferrine. Microbial siderophores of the hydroxamate and phenolate types, such as desferrioxamin B and enterobactine as well as other iron chelators (phenanthroline, citrate and nitrilotriacetate) decrease the bacterial cell growth or iron incorporation and are not relevant for iron transport in P. multocida. The direct analytical identification of siderophores using the reactions by Csaky (hydroxamate type) and Arnow (phenolate type) has proved unsuccessful. The importance of the mannan cell wall polysaccharide is discussed with respect to the iron transport. Thus in terms of iron accumulation, P. multocida is similar to Yersinia, which also possess an efficient transport system for iron not involving siderophores.

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Beeinflussung von Virulenz und Immunogenität beiPasteurella multocida durch Eisenin vitro

Cited by 9 publications

References 22 publications

Brackiella oedipodis gen. nov., sp. nov., gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus).

Brackiella oedipodis gen. nov., sp. nov., gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus).

Untersuchungen zur Oberfläuchenstruktur von Pasteurella multocida

Nachweis von Eisen‐Transport‐Aktivität in Pasteurella multocida‐Kulturen

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