Beech wood Fagus sylvatica dilute-acid hydrolysate as a feedstock to support Chlorella sorokiniana biomass, fatty acid and pigment production

Miazek, Krystian; Remacle, Claire; Richel, Aurore; Goffin, Dorothée

doi:10.1016/j.biortech.2017.01.034

Cited by 24 publications

(25 citation statements)

References 45 publications

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“…Beech wood material contained 44% ± 2 glucose, 17% ± 1 xylose and 20% ± 1 Klason lignin as it was determined in our previous report, where wood particles were subjected to a diluted acid (3%) treatment (H 2 SO 4 ) [13]. However, solids collected after the diluted acid treatment were enzymatically hydrolysed with low (<5%) saccharification rate, and concentration of produced glucose was insufficient as a feedstock for microalgae cultures (data not shown).…”

Section: Pretreatment and Enzymatic Hydrolysis Of Woodmentioning

confidence: 98%

“…Content of chlorophyll a, chlorophyll b and total carotenoids in Chlorella biomass was determined spectrophotometrically as reported previously [13]. Fatty acid and pigment content in biomass was expressed on dry weight (%) basis.…”

Section: Fame and Pigment Determination In Microalgae Biomassmentioning

confidence: 99%

“…Remained solids were rinsed with distilled water (to remove slurry remnants) and collected for content analysis and enzymatic hydrolysis. Wood particles were milled into powder and analyzed for sugar (glucose, xylose) and Klason lignin content, as reported previously [13]. Pretreated wood solids were enzymatically hydrolyzed by Celluclast ® 1.5 L (cellulase mixture) and Novozym 188 (cellobiase) (Novozymes A/S Denmark) [16], at a dose level of 0.16 mL cellulase/g pretreated solids and 0.08 mL cellobiase/g pretreated solids.…”

Section: Wood Alkaline Pretreatment and Enzymatic Hydrolysismentioning

confidence: 99%

“…Fatty acids in Chlorella biomass were extracted and converted into Fatty Acid Methyl Esters (FAMEs) and measured by Gas Chromatography, as reported previously [13]. Content of chlorophyll a, chlorophyll b and total carotenoids in Chlorella biomass was determined spectrophotometrically as reported previously [13].…”

Section: Fame and Pigment Determination In Microalgae Biomassmentioning

confidence: 99%

“…Hence, in this study, an enzymatic hydrolysate from alkali pretreated beech Fagus sylvatica wood was tested as a feedstock for Chlorella cultures. Beech (Fagus sylvatica) is a deciduous tree species spread around Europe, also commonly found in Belgium [11], and was a subject of hydrolysis studies in our previous reports [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

et al. 2017

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This work evaluates the possibility of applying enzymatic beech wood (Fagus sylvatica) hydrolysate as a feedstock for Chlorella sorokiniana growth, and fatty acid and pigment production. Beech wood solids were pretreated with NaOH at high temperature to partially remove xylose and Klason lignin, and enable production of glucose during subsequent enzymatic hydrolysis. Neutralized wood enzymatic hydrolysate containing glucose (TGP-Enz10), was tested on Chlorella growth during heterotrophic cultivation and compared with microalgae growth in a medium containing synthetic glucose (TGP). Results show that enzymatic hydrolysate enabled Chlorella growth in the dark for biomass, fatty acid and pigment production due to the presence of glucose, although the productivity obtained was smaller, if compared to heterotrophic cultivation in a synthetic TGP medium. Partial growth inhibition and diminished productivity in wood hydrolysate supplemented Chlorella culture was due to the presence of neutralized citrate buffer. Neutralized citrate buffer (TGP-Cit10) was found to partially inhibit heterotrophic growth and also strongly suppress mixotrophic growth in Chlorella culture. This buffer was also shown to alter fatty acid composition and to slightly affect Chl Total /Car Total ratio during heterotrophic cultivation. Heterotrophic Chlorella cultivation with TGP-Enz10 showed that wood enzymatic hydrolysate can constitute a potential feedstock for microalgae cultivation, although the composition of the buffer used during enzymatic hydrolysis should be taken into consideration.

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Section: Pretreatment and Enzymatic Hydrolysis Of Woodmentioning

confidence: 98%

Section: Fame and Pigment Determination In Microalgae Biomassmentioning

confidence: 99%

Section: Wood Alkaline Pretreatment and Enzymatic Hydrolysismentioning

confidence: 99%

Section: Fame and Pigment Determination In Microalgae Biomassmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

et al. 2017

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Transformation of rice bran into single‐cell protein, extracted protein, soluble and insoluble dietary fiber, and minerals

2019

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BACKGROUND It is evident that, during the conversion of agricultural sidestreams into valuable substances, a complete utilization is necessary for economic reasons. The present study investigated the transformation of defatted rice bran into proteins (single cell and extracted protein), soluble and insoluble dietary fiber, and minerals. RESULTS In a process chain, starch/glucose was enzymatically extracted and converted into single cell protein (Chlorella sorokiniana). Then, rice bran proteins were extracted and partially precipitated. The remaining liquid was ultrafiltered (3 kDa) to obtain a further protein fraction and minerals. The protein fraction contained a considerable amount of soluble dietary fiber. With these steps, around 69% of the rice bran was transformed, resulting in three protein fractions with an average purity of approximately 45% and minerals with a purity of approximately 63%. In a subsequent process, the remaining cake was disintegrated at 95 °C and pH 2. A further 12% of the rice bran could be liquefied. After centrifugation, the supernatant was subjected to ultrafiltration (3 kDa) to obtain soluble dietary fiber in the retentate and minerals in the permeate. However, only around 2% of the rice bran was converted into soluble dietary fiber, whereas the remainder comprised a mixture of minerals and monomeric sugars. CONCLUSION The process chain can be rearranged and optimized especially to increase the output of soluble dietary fiber and proteins as a result of using the digested monomeric sugars for algae cultivation. At the end of the process, 18% of rice bran remained as an insoluble dietary fiber fraction. © 2019 Society of Chemical Industry

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Investigation of Silphium perfoliatum as Feedstock for a Liquid Hot Water–Based Biorefinery Process Towards 2,3-Butanediol

et al. 2020

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Beech wood Fagus sylvatica dilute-acid hydrolysate as a feedstock to support Chlorella sorokiniana biomass, fatty acid and pigment production

Cited by 24 publications

References 45 publications

Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

Effect of Enzymatic Beech Fagus Sylvatica Wood Hydrolysate on Chlorella Biomass, Fatty Acid and Pigment Production

Transformation of rice bran into single‐cell protein, extracted protein, soluble and insoluble dietary fiber, and minerals

Investigation of Silphium perfoliatum as Feedstock for a Liquid Hot Water–Based Biorefinery Process Towards 2,3-Butanediol

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