Bee Venom Phospholipase Inhibits Malaria Parasite Development in Transgenic Mosquitoes

Moreira, Luciano Andrade; Ito, Junitsu; Ghosh, Anil K.; Devenport, Martin; Zieler, Helge; Abraham, Eappen G.; Crisanti, Andrea; Nolan, Tony; Catteruccia, Flaminia; Jacobs-­Lorena, Marcelo

doi:10.1074/jbc.m206647200

Cited by 187 publications

(139 citation statements)

References 20 publications

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“…For insects, the most important transposable elements for their transformation success were: Hermes from Musca domestica L. (Warren et al 1994) used to transform A. aegypti (Jasinskiene et al 1998, Kokoza et al 2000, Moreira et al 2000, Pinkerton et al 2000, mariner or Mos1 from Drosophila mauritiana Tsacas & David (Medhora et al 1991) for A. aegypti transformation (Coates et al 1998, Moreira et al 2000; Minos from Drosophila hydei Sturtevant (Franz & Savakis, 1991) used to transform Anopheles stephensi Liston (Catteruccia et al 2000) and piggyBac from Trichoplusia ni Hübner (Cary et al 1989) that has been shown to be very promiscuous in a number of insect orders and also mosquito species [ (Ito et al 2002, Moreira et al 2002, for A. gambiae (Grossman et al 2001) and more recently for Anopheles albimanus Wiedemann (Perera et al 2002)]. …”

Section: Overview Of the Mosquito Transformationmentioning

confidence: 99%

“…Besides being universal, GFP has the advantage that transformants can already be detected at larval stages. GFP constructs driven by the polyubiquitin (Handler & Harrell 1999, Perera et al 2002 and PAX eye-specific (Horn et al 2000, Kokoza et al 2001, Ito et al 2002, Moreira et al 2002 promoters have also been tested. Among these, the eyespecific promoter is more suitable because as the GFP expression occurs only in eye tissues it may reduce possible toxic effects of this protein (Fig.…”

Section: How To Detect Transformants?mentioning

confidence: 99%

“…1). GFP is being the most preferred marker and has been used for A. aegypti (Pinkerton et al 2000, Kokoza et al 2001, C. quinquefasciatus (Allen et al 2001), A. stephensi (Catteruccia et al 2000, Ito et al 2002, Moreira et al 2002), A. gambiae (Grossman et al 2001 and A. albimanus (Perera et al 2002).…”

Section: How To Detect Transformants?mentioning

confidence: 99%

“…The next step was to construct a hybrid gene to express a SM1 tetramer in transgenic mosquitoes. Ito et al (2002) used the carboxypeptidase promoter and its secretory signal sequence to drive this tetramer in transgenic A. stephensi. The transformed mosquitoes indeed expressed the transgene and effectively inhibited oocyst formation (69% to 95%) after feeding on infected mice.…”

Section: Anti-parasitic Genesmentioning

confidence: 99%

“…The mechanism of action was unclear but probably the venom PLA2s, with their membrane inserting capability, would mask the receptors on the midgut epithelia that the parasite uses to recognize and invade it. Using one of this anti-parasite molecule Moreira et al (2002) transformed A. stephensi with a construct similar to the one used by Ito et al (2002) but instead of the SM1 tetramer they placed the bee venom PLA2 coding region. They detected PLA2 mRNA specifically in the guts of transgenic mosquitoes, what would be expected when using a gut specific promoter.…”

Section: Anti-parasitic Genesmentioning

confidence: 99%

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Transgenic mosquitoes for malaria control: progresses and challenges

Moreira¹,

Jacobs-Lorena²

2003

Neotrop. Entomol.

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Mosquitos Transgênicos Para o Controle da Malária: Progressos e DesafiosRESUMO -A malária mata milhões de pessoas a cada ano e as estratégias atuais de controle da doença, como inseticidas e drogas não têm sido tão eficientes. Por este motivo, novos meios para o combate à malária são de extrema importância. Avanços no estudo do mosquito vetor e sua interação com o parasito da malária fizeram os cientistas pensarem que é possível a manipulação genética dos mosquitos para torná-los vetores ineficientes. Neste artigo, revisamos os avanços na introdução de genes exógenos na linhagem germinativa de mosquitos, a caracterização de promotores específicos de certos tecidos, a identificação de produtos gênicos que bloqueiam o parasita no mosquito, bem como discutimos a recente geração de mosquitos transgênicos, menos eficientes na transmissão de malária. Enquanto muitos progressos foram obtidos, muitos anos de pesquisa são ainda necessários para que mosquitos transgênicos possam ser utilizados na natureza. PALAVRAS-CHAVE: Malária, transgenia, bloqueio da transmissão, PlasmodiumABSTRACT -Malaria kills millions of people every year and the current strategies to control the disease, such as insecticides and drugs have not been completely efficient. Because of that, novel means to fight against malaria are of utmost importance. Advances in the study of the mosquito vector and its interactions with the malaria parasite made scientists think that it is possible to genetically manipulate the mosquitoes to make them inefficient vectors. Here we review the advances on the introduction of foreign genes into the mosquito germ line, the characterization of tissue-specific promoters, the identification of gene products that block development of the parasite in the mosquito, and we discuss the recent generation of transgenic mosquitoes impaired for malaria transmission. While much progress has been made, many years of research are still needed before transgenic mosquitoes can be used in the field.

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Section: Overview Of the Mosquito Transformationmentioning

confidence: 99%

Section: How To Detect Transformants?mentioning

confidence: 99%

Section: How To Detect Transformants?mentioning

confidence: 99%

Section: Anti-parasitic Genesmentioning

confidence: 99%

Section: Anti-parasitic Genesmentioning

confidence: 99%

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Transgenic mosquitoes for malaria control: progresses and challenges

Moreira¹,

Jacobs-Lorena²

2003

Neotrop. Entomol.

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Genomics spawns novel approaches to mosquito control

et al. 2003

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In spite of advances in medicine and public health, malaria and other mosquito-borne diseases are on the rise worldwide. Although vaccines, genetically modified mosquitoes and safer insecticides are under development, herein we examine a promising new approach to malaria control through better repellents. Current repellents, usually based on DEET, inhibit host finding by impeding insect olfaction, but have significant drawbacks. We discuss how comparative genomics, using data from the Anopheles genome project, allows the rapid identification of members of three protein classes critical to insect olfaction: odorant-binding proteins, G-protein-coupled receptors, and odorant-degrading enzymes. A rational design approach similar to that used by the pharmaceutical industry for drug development can then be applied to the development of products that interfere with mosquito olfaction. Such products have the potential to provide more complete, safer and longer lasting protection than conventional repellents, preventing disease transmission by interrupting the parasite life cycle.

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Malaria vector control in the third millennium: progress and perspectives of molecular approaches

Catteruccia

2007

Pest Management Science

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Remarkable progress has been made towards a deeper understanding of mosquito biology since the completion of the Anopheles gambiae Giles genome project. Combined with the development of efficient transgenic technologies for genetic modification of major vector species and the availability of powerful molecular, genetic and bioinformatics tools, this is allowing the identification of genes involved in mosquito biological functions crucial to malaria transmission, ranging from host-seeking behaviour and innate immunity to insecticide resistance. Moreover, population genetic studies are beginning to elucidate the complex structure of vector populations. Finally, novel methods for malaria control are emerging that are based on the use of genetically modified mosquitoes either to interrupt the journey of the Plasmodium parasite within its insect host or to suppress those mosquito species that function as vectors for parasite transmission.

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Bee Venom Phospholipase Inhibits Malaria Parasite Development in Transgenic Mosquitoes

Cited by 187 publications

References 20 publications

Transgenic mosquitoes for malaria control: progresses and challenges

Transgenic mosquitoes for malaria control: progresses and challenges

Genomics spawns novel approaches to mosquito control

Malaria vector control in the third millennium: progress and perspectives of molecular approaches

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