Bee venom induces the interaction between phosphorylated histone variant, γH2AX, and intracellular location of beta actin in cancer cells

Abstract

Bee venom is a natural compound and candidate anti-cancer agent with selective cytotoxic effect on some cancer cells. However, the cellular mechanisms of how bee venom selectively targets cancer cells remain elusive. The aim of this study was to reveal the genotoxic effect of bee venom in concordance with the location of β-actin protein throughout the nucleus or/and cytoplasm. For this aim, the level of H2AX phosphorylation (γH2AX) and intracellular location of β-actin were assessed by immunofluorescence in liver (HEPG2) and metastatic breast (MDA-MB-231) cancer cell lines compared to normal fibroblasts (NIH3T3) after bee venom. Co-localisation of γH2AX and β-actin were also analysed. The results showed that the levels of γH2AX staining decreased in normal cells but increased in cancer cells. Majority of β-actin was localised within the cytoplasm of normal cells after bee venom, but it was mostly accumulated within the nucleus in cancer cells. Co-localisation of β-actin and γH2AX both in nucleus and cytoplasm was induced in each cell by different patterns. The results showed that normal and cancerous cells had different responses against bee venom, and suggested that bee venom induced a cellular response by the interaction between γH2AX and β-actin.