Bee-safe peptidomimetic acaricides achieved by comparative genomics

Jindal, Vikas; Li, Daqi; Rault, Leslie C.; Fatehi, Soheila; Singh, Rupinder; Mating, Moritz; Zou, Ye; Ng, Ho Leung; Kaczmarek, Krzysztof; Zabrocki, Janusz; Gui, Shun‐Hua; Smagghe, Guy; Anderson, Troy D.; Nachman, Ronald J.; Park, Yoonseong

doi:10.1038/s41598-022-20110-0

Cited by 6 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, a neuropeptide only present in pests and absent in beneficial insects may also provide a highly selective pesticide target (Jindal et al. , 2022). In the current study, we compared the differences in neuropeptide precursor genes between G. mellonella and honeybees.…”

Section: Discussionmentioning

confidence: 99%

Genomics, transcriptomics, and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress

Luo,

Lin,

Linghu

et al. 2023

Insect Science

Self Cite

View full text Add to dashboard Cite

Neuropeptides are crucial in regulation of a rich variety of developmental, physiological, and behavioral functions throughout the life cycle of insects. Using an integrated approach of multiomics, we identified neuropeptide precursors in the greater wax moth Galleria mellonella, which is a harmful pest of honeybee hives with a worldwide distribution. Here, a total of 63 and 67 neuropeptide precursors were predicted and annotated in the G. mellonella genome and transcriptome, in which 40 neuropeptide precursors were confirmed in the G. mellonella peptidome. Interestingly, we identified 12 neuropeptide precursor genes present in G. mellonella but absent in honeybees, which may be potential novel pesticide target sites. Honeybee hives were contaminated with heavy metals such as lead, enabling its bioaccumulation in G. mellonella bodies through the food chain, we performed transcriptome sequencing to analyze the effects of Pb stress on the mRNA expression level of G. mellonella neuropeptide precursors. After treatment by Pb, the expression of neuropeptide F1 was found to be significantly downregulated, implying that this neuropeptide might be associated with responding to the heavy metal stress in G. mellonella. This study comprehensively identified neuropeptide precursors in G. mellonella, and discussed the effects of heavy metals on insect neuropeptides, with the example of G. mellonella. The results are valuable for future elucidation of how neuropeptides regulate physiological functions in G. mellonella and contribute to our understanding of the insect's environmental plasticity and identify potential new biomarkers to assess heavy metal toxicity in insects.

show abstract

Section: Discussionmentioning

confidence: 99%

Genomics, transcriptomics, and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress

Luo,

Lin,

Linghu

et al. 2023

Insect Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Honey bee bacterial symbionts have recently been produced to express dsRNA for both DWV and varroa control (Leonard et al, 2020). Another bee-safe approach might be through selective acaricides targeting the varroa mite-specific neuropeptides and signaling systems (Jindal et al, 2022). These biopesticides and soft acaricides offer an opportunity for enhancing varroa IRM, as they could reduce acaricide selection pressure and complement synthetic pesticide control efforts (Georghiou, 1994).…”

Section: Varroa Irm By Moderationmentioning

confidence: 99%

Integrated resistance management for acaricide use on Varroa destructor

Lester

2023

Front. Bee Sci.

View full text Add to dashboard Cite

The global beekeeping industry faces an escalating challenge in the form of Varroa destructor. Synthetic chemicals serve as a cornerstone for varroa management, although they face a major challenge in the form of acaricide resistance. Here, I examine acaricide resistance in varroa under the framework of Insecticide Resistance Management (IRM). I assess the potential of diverse IRM strategies, such as pesticide rotation and mixtures, refuge utilization, synergists and the integration of non-persistent chemicals. The peculiar life history of varroa, characterized by its incestuous breeding system, challenges conventional IRM strategies. There is little published evidence that pesticide rotation is beneficial for resistance management in varroa, with several studies showing resistance is maintained despite rotation. Fitness costs associated with pesticide resistance are often an essential component for IRM strategies, but there are no current data from varroa demonstrating such specific fitness costs (e.g., a reduced relative oviposition rate) associated with resistance. The single published experimental study directly examining relative fitness found that here was little or no reproductive fitness cost associated with pyrethroid resistance. More work is needed on fitness effects of the key acaricides, which would better guide the use of rotation and refuge strategies. A key prospect for future work that has been identified through simulation modeling is offered by pesticide mixtures and the role of synergists to elevate acaricide efficacy. Additional tools for varroa IRM include ‘soft’ acaricides, including oxalic acid, and biopesticides such as dsRNA. In light of the widespread prevalence of acaricide resistance and an increasing varroa problem, there is an urgent need for nuanced, data-driven varroa IRM strategies.

show abstract

“…Because our enzyme of interest belongs to the group of enzymes utilizing cofactors (NADP + ), we decided to characterize the interacting sites using an established approach [33]. By combining homology modeling and docking, with mutagenesis of the identified active site, the importance of the main amino acids essential for the enzymatic reaction can be confirmed.…”

Section: Introductionmentioning

confidence: 99%

The Active Site of the Enzyme 10-Formyl-THFDH in the Honey Bee Apis mellifera—A Key Player in Formic Acid Detoxification

Mating

Zou

Sharbati

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Honey bees are important managed pollinators that fulfill important ecological and economic functions. In recent decades, the obligate ectoparasite Varroa destructor severely affected the survival of honey bees, as it weakened them by different means. A common treatment against V. destructor is formic acid fumigation, which has been used for decades by beekeepers across the world. This treatment is known to be effective, but many beekeepers report adverse effects of formic acid on bees, which include damage to the brood, worker bee mortality, and queen loss. Little is known about the molecular mechanisms of formic acid detoxification in honey bees. Recently, we reported upregulation of the bee enzyme, 10-formyl-THFDH, under formic acid fumigation. Here, the active site of this enzyme is characterized by an interdisciplinary approach combining homology modeling and protein mutagenesis. In addition, the limitations of the 3D protein structure prediction program AlphaFold2 are shown in regard to docking studies. This study provides a more thorough understanding of the molecular detoxification mechanisms of formic acid in Apis mellifera.

show abstract

Bee-safe peptidomimetic acaricides achieved by comparative genomics

Cited by 6 publications

References 54 publications

Genomics, transcriptomics, and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress

Genomics, transcriptomics, and peptidomics of the greater wax moth Galleria mellonella neuropeptides and their expression in response to lead stress

Integrated resistance management for acaricide use on Varroa destructor

The Active Site of the Enzyme 10-Formyl-THFDH in the Honey Bee Apis mellifera—A Key Player in Formic Acid Detoxification

Contact Info

Product

Resources

About