Beckman Coulter and CD4+ T cells

Rabellino, Enrique M.; Forman, Meryl A.; Howes, Grant C.J.; Mills, Rhonda; Quintana, Jorge A.; Roth, Patricia; Wilkinson, Julie

doi:10.1002/cyto.10096

Cited by 4 publications

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“…THE absolute CD4 1 T-lymphocyte count (CD4 count) serves as the major clinical indicator of immunocompetence in patients with HIV infection (1,2). It is considered to be the best surrogate marker for the monitoring of the clinical course of the infection with HIV, as these cells are the natural host to the virus.…”

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Low‐cost enumeration of CD4⁺ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries

et al. 2007

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The CD4 count is the best surrogate marker for monitoring HIV. The reference method for assessing CD4 counts (flow cytometry, FCM), as expensive technique, is not widely used in non-OECD countries. To make HIV-monitoring available to more patients in these countries, we modified a commercially available density-based negative selection assay (RosetteSep TM ) to make it applicable for low-cost cell enumeration. For evaluation (Step 1), blood taken from 25 HIV patients and 29 healthy donors was assayed with the modified negative selection method (MNS) and compared with FCM. For validation (Step 2), this method was performed in blind quintuplicates on 12 HIVþ blood samples according to FDA guidelines. Association of MNS with the FCM is given by regression models for both steps:Step 1: slope = 1.091, intercept = À46.5.Step 2: slope = 1.074, intercept = À38.3 (Step 2). The imprecision of MNS assessed during Step 2 was 21.2% (intraserial) and 18.8% (interserial). The results suggest that MNS is capable of providing an approximate CD4 count. At a cost of 0.30, it is affordable to patients living in resource-restrained areas. The technique has the potential to deliver an accurate, precise, low-cost test to monitor HIVþ patients. ' 2007 International Society for Analytical Cytology

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Low‐cost enumeration of CD4⁺ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries

et al. 2007

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Microchip-Based Assay Systems

Floriano¹

2007

Methods in Molecular Biology

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Microchip-Based Enumeration of Human White Blood Cells

Floriano

Acosta

Christodoulides

et al. 2007

Methods in Molecular Biology

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The advent of flow cytometry has considerably changed the ways in which medical testing is conducted. However, the cost of flow cytometers, their large size, and their maintenance needs make them scarce in resource-poor settings and available almost only in clinical pathology laboratories in developed countries. Because cell enumeration is a basic and crucial support of diagnosis, prognosis, and treatment, an alternative cell-counting method that would potentially be cost-effective, portable, and suitable for use in resource-poor settings is warranted. We describe here a protocol for conducting cell-counting experiments in a simple microfluidic structure. This protocol describes how to build a simple microfluidic cell and perform a total white blood cell (WBC) count through capture and immunolabeling of the WBCs with an anti-CD45 antibody.

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Beckman Coulter and CD4+ T cells

Cited by 4 publications

References 0 publications

Low‐cost enumeration of CD4⁺ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries

Low‐cost enumeration of CD4⁺ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries

Microchip-Based Assay Systems

Microchip-Based Enumeration of Human White Blood Cells

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Beckman Coulter and CD4+ T cells

Cited by 4 publications

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Low‐cost enumeration of CD4+ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries

Low‐cost enumeration of CD4+ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries

Microchip-Based Assay Systems

Microchip-Based Enumeration of Human White Blood Cells

Contact Info

Product

Resources

About

Low‐cost enumeration of CD4⁺ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries

Low‐cost enumeration of CD4⁺ T cells using a density‐based negative selection method (RosetteSep™) for the monitoring of HIV‐infected individuals in non‐OECD countries