BEAP profiles as rapid test system for status analysis and early detection of process incidents in biogas plants

Refai, Sarah; Berger, Stefanie; Wassmann, Kati; Hecht, Melanie; Dickhaus, Thomas; Deppenmeier, Uwe

doi:10.1007/s10295-016-1894-8

Cited by 3 publications

(3 citation statements)

References 59 publications

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“…VFAs are key intermediates in the anaerobic degradation and their concentrations are important parameters in monitoring the state of biogas reactors (Hansen et al, 1998; Weiland, 2010; Lv et al, 2014). The individual degradation of VFAs such as acetate, butyrate, and propionate under different conditions has been in the focus of different studies, as their metabolization can give important information on the fitness of the microbial community (Refai et al, 2017). A clear difference was observed in the acetate and butyrate concentrations between the two setups (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

“…Besides physiochemical parameters such as VFA concentrations (Moestedt et al, 2016) and the ability to degrade intermediates (Refai et al, 2017), CH 4 formation can be used to monitor the productivity of a reactor (Ziganshin et al, 2013; Refai et al, 2017) as it is a final product of the anaerobic degradation. Thus, lower CH 4 yield points to a less effective degradation and possibly toward an imbalanced system, in our case as a result of increased ammonia concentration.…”

Section: Resultsmentioning

confidence: 99%

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Immediate Effects of Ammonia Shock on Transcription and Composition of a Biogas Reactor Microbiome

et al. 2019

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The biotechnological process of biogas production from organic material is carried out by a diverse microbial community under anaerobic conditions. However, the complex and sensitive microbial network present in anaerobic degradation of organic material can be disturbed by increased ammonia concentration introduced into the system by protein-rich substrates and imbalanced feeding. Here, we report on a simulated increase of ammonia concentration in a fed batch lab-scale biogas reactor experiment. Two treatment conditions were used simulating total ammonia nitrogen concentrations of 4.9 and 8.0 g/L with four replicate reactors. Each reactor was monitored concerning methane generation and microbial composition using 16S rRNA gene amplicon sequencing, while the transcriptional activity of the overall process was investigated by metatranscriptomic analysis. This allowed investigating the response of the microbial community in terms of species composition and transcriptional activity to a rapid upshift to high ammonia conditions. Clostridia and Methanomicrobiales dominated the microbial community throughout the entire experiment under both experimental conditions, while Methanosarcinales were only present in minor abundance. Transcription analysis demonstrated clostridial dominance with respect to genes encoding for enzymes of the hydrolysis step (cellulase, EC 3.2.1.4) as well as dominance of key genes for enzymes of the methanogenic pathway (methyl-CoM reductase, EC 2.8.4.1; heterodisulfide reductase, EC 1.8.98.1). Upon ammonia shock, the selected marker genes showed significant changes in transcriptional activity. Cellulose hydrolysis as well as methanogenesis were significantly reduced at high ammonia concentrations as indicated by reduced transcription levels of the corresponding genes. Based on these experiments we concluded that, apart from the methanogenic archaea, hydrolytic cellulose-degrading microorganisms are negatively affected by high ammonia concentrations. Further, Acholeplasma and Erysipelotrichia showed lower abundance under increased ammonia concentrations and thus might serve as indicator species for an earlier detection in order to counteract against ammonia crises.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Immediate Effects of Ammonia Shock on Transcription and Composition of a Biogas Reactor Microbiome

et al. 2019

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“…This led to decreasing pH values. These variation in pH cycles in a biogas system are normal and have been used as an indicator to determine the status of biogas production [26]. It is however worth noting that these cycles did not limit continuous biogas production.…”

Section: Substrate Phmentioning

confidence: 99%

The Influence of Biocatalytic Plant Extracts on Biogas Production from Kitchen Wastes at Cryo-mesophilic Temperature Regimes

Chaka¹,

Aloys²,

Maghanga³

et al. 2019

IJEEE

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Radicalization in waste-to-energy systems are on the rise to meet human energy demands. Biogas generation from kitchen wastes is one such scheme, though affected by poor yields and methane levels at low temperatures. In this research, biocatalytic extracts with fermentative properties were hereby assessed on their potential to fasten these processes and increase the biogas yield at ambient temperatures. The variations in kitchen waste substrate anaerobic parameters and elemental composition as well as biogas yields and methane levels were monitored in a 28-day retention period. Three 40-liter batch and unstirred bio-digesters containing biocatalysts Terminalia b., Acanthaceae spp. and a control setup were used. The results indicated rapid saccharification rates in the samples with additives. Terminalia b. additives exhibited high volatile solids hydrolysis rate of 98.3% followed by Acanthaceae spp. (50.8%) and control sample (29.4%). Similar trends were observed in organic carbon reduction as the levels of nitrogen, phosphorus and sulfur linearly increased. The biocatalysts did not affect substrate pH, volatile fatty acids and alkalinity levels. Terminalia b. sample produced 2.32 folds higher while Acanthaceae spp. sample produced 1.375 folds higher than the control sample. Terminalia b. methane levels were highest (45.475±0.922%) followed by the control sample (41.750±1.401) and Acanthaceae spp. sample (39.275±0.263%) after 28-day retention period at 19.5±0.5°C. Use of these biocatalysts in biofuel synthesis can thus optimize biogas production leading to greener economies.

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Response of aquatic plant decomposition to invasive algal organic matter mediated by the co-metabolism effect in eutrophic lakes

Deng

Yan

et al. 2023

Journal of Environmental Management

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BEAP profiles as rapid test system for status analysis and early detection of process incidents in biogas plants

Cited by 3 publications

References 59 publications

Immediate Effects of Ammonia Shock on Transcription and Composition of a Biogas Reactor Microbiome

Immediate Effects of Ammonia Shock on Transcription and Composition of a Biogas Reactor Microbiome

The Influence of Biocatalytic Plant Extracts on Biogas Production from Kitchen Wastes at Cryo-mesophilic Temperature Regimes

Response of aquatic plant decomposition to invasive algal organic matter mediated by the co-metabolism effect in eutrophic lakes

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