Bean (Phaseolus vulgaris L.) polysaccharides modulate gene expression in human colon cancer cells (HT-29)

The fermented non-digestible fraction of common bean (Phaseolus vulgaris L.) triggers cell cycle arrest and apoptosis in human colon adenocarcinoma cells

“…Interestingly, this gene was the most down-regulated by both hgm-FNDF-LC 50 and SCFA-LC 50 treatments in our study (-84.45-and -42.62-fold, respectively; Table 2). A similar effect was observed by Campos-Vega et al (2010) after treatment by the fermented polysaccharide extract from common bean cultivar Bayo Madero in HT-29 cells, suggesting the modulation of mutated p53 as an inhibiting mechanism for non-digestible compounds of common bean in colon adenocarcinoma cells, and probably dependent of the cultivar since the effect of the hgm-FNDF-LC 50 on this gene expression was in a lesser extent (-4.8).…”

“…Genes Nutr (2014) 9:359 Page 7 of 12 359 cells were treated with a fermented polysaccharide extract of common bean (P. vulgaris L.) cultivar Bayo Madero (Campos-Vega et al 2010), supporting the suggesting inhibiting effect on cell cycle progression by derivative compounds of common bean fermentation. SIRT1 belongs to sirtuin proteins with NAD?-dependent deacetylase activity; since this deacetylase can down-regulate the activity of NFjB, it can also be considered as anti-proliferative (Wolf et al 2011).…”

“…Furthermore, BCL2A1 expression can be induced by NFjB, and it contains a binding site for NFjB stimulating its activity (Chen et al 2004). The inhibition of this gene by antitumor compounds in foods including common bean has been previously demonstrated (FeregrinoPérez et al 2008;Campos-Vega et al 2010). …”

“…Genetically, HT-29 cells present typical changes in colorectal tumors, such as APC, K-ras and p53 mutations and the consequent loss of their function, as well as amplification of c-myc (Rodrigues et al 1990;Choi et al 2008;Zhang et al 2009;Wu et al 2010) and a phenotype of chromosomal instability and aneuploidy. This cell line has been used as a model to investigate the mechanism by which some protective compounds sensitize HT-29 cells to control of proliferation and apoptosis (Beyer-Sehlmeyer et al 2003;Daly et al 2005;Campos-Vega et al 2010).…”

“…We previously demonstrated that compounds of common bean can inhibit proliferation and induce apoptosis in both in vivo and in vitro models by modulating the expression of genes involved in cell cycle arrest, cell proliferation and apoptosis (Feregrino-Pérez et al 2008;Campos-Vega et al 2010). Our previous study showed that common bean (Phaseolus vulgaris L.) Negro 8025 has fermentable substrates, such as the NDF (total dietary fiber, oligosaccharides, resistant starch and phenolic compounds), and the NDF was submitted to gut in vitro fermentation producing compounds that showed chemoprotective effect by inducing DNA fragmentation (apoptosis) and inhibiting the HT-29 cell survival (CruzBravo et al 2011).…”