Bean and Pea Plastoglobules Change in Response to Chilling Stress

Abstract

Plastoglobules (PGs) might be characterised as microdomains of the thylakoid membrane that serve as a platform to recruit proteins and metabolites in their spatial proximity in order to facilitate metabolic channelling or signal transduction. This study provides new insight into changes in PGs isolated from two plant species with different responses to chilling stress, namely chilling-tolerant pea (Pisum sativum) and chilling-sensitive bean (Phaseolus coccineus). Using multiple analytical methods, such as high-performance liquid chromatography and visualisation techniques including transmission electron microscopy and atomic force microscopy, we determined changes in PGs’ biochemical and biophysical characteristics as a function of chilling stress. Some of the observed alterations occurred in both studied plant species, such as increased particle size and plastoquinone-9 content, while others were more typical of a particular type of response to chilling stress. Additionally, PGs of first green leaves were examined to highlight differences at this stage of development. Observed changes appear to be a dynamic response to the demands of photosynthetic membranes under stress conditions.

Association of jasmonic acid priming with multiple defense mechanisms in wheat plants under high salt stress

“…This confirms that the pre-treatment of JA plays an important role in preventing chloroplast degradation by enhancing the production of protective antioxidant compounds (e.g., α-tocopherols) which tend to conserve membrane against ROS (Bashandy et al, 2020 ; Younes et al, 2020 ) and hinder photoinactivation of PSII (Havaux et al, 2005 ). In this regard, plastoglobules are a sink of lipophilic antioxidants such as α-tocopherol where main enzymes have been present in their membranes (Wójtowicz et al, 2021 ). The data of current work found that α-tocopherol content elevated parallel to a higher number of plastoglobules as was reported by Wójtowicz et al ( 2021 ).…”

“…In this regard, plastoglobules are a sink of lipophilic antioxidants such as α-tocopherol where main enzymes have been present in their membranes (Wójtowicz et al, 2021 ). The data of current work found that α-tocopherol content elevated parallel to a higher number of plastoglobules as was reported by Wójtowicz et al ( 2021 ). Thus, JA instigates cellular signaling under salinity stress through mediating α-tocopherol content.…”

Availability of iron ions impacts physicochemical properties and proteome of outer membrane vesicles released by Neisseria gonorrhoeae

“…AFM measurements were performed with an Agilent 5500 microscope (Agilent, Santa Clara, CA, USA), working in contact and tapping mode, under the control of PicoView software as previously described 53 . OMVs were immobilized on freshly cleaved mica, with poly-L-lysine as a stabilizing matrix.…”

“…Other parameters were used as pre-set in the software. Unique and razor peptides were used for quantification enabling protein grouping (razor peptides are the peptides uniquely assigned to protein groups and not to individual proteins) 53 – 55 .…”

Estimation of Biochemical Compounds in Tradescantia Leaves Using VIS-NIR-SWIR Hyperspectral and Chlorophyll a Fluorescence Sensors

“…Furthermore, PCA can be combined with regression coefficients obtained from various spectral curves, such as OJIP, which represent the fluorescence kinetics over the first few seconds of illumination, and hyperspectral reflectance, transmittance, and absorbance curves. This combination allows for more comprehensive analysis by combining biochemical and biophysical insights [31]. Such a multifaceted approach enhances the understanding of plant responses and interactions with their environment, aiding in more accurate and predictive modelling of plant physiology and agronomy by remote-sensing techniques [4][5][6]32].…”