Beam propagation analysis on thickness measurements in quantitative phase microscopy

Bae, Yoon Sung; Song, Jung‐Han; Har, Dongsoo; Kim, Dug Young

doi:10.1007/s10043-015-0112-7

Cited by 1 publication

(1 citation statement)

References 23 publications

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“…Now, to obtain the phase-map of each pixel, probe interferograms reflected from a trapped RBC were captured at a CCD camera and analysed by algorithms written in MATLAB. No reflection can occur from the intermediate cytoplasmic medium because it is transparent for visible wavelength of probe light (632 nm) thereby assuming no wavefront aberration from phase object (RBC) [80]. Total OPD between two co-propagating reflected interferograms: one from the top surface and other from the bottom surface of RBC (after transmission from the top surface) is twice the thickness of discotic RBC (0.9 µm -2.5 µm) i.e., 1.8 µm -5µm.…”

Section: Role Of Arbitrary Intensity Profile Beam In Trapping and mentioning

confidence: 99%

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

Kumar

Srivastava

Mehta

et al. 2016

Journal of the Optical Society of Korea

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Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of 'arbitrary intensity profile' generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC's membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

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Section: Role Of Arbitrary Intensity Profile Beam In Trapping and mentioning

confidence: 99%

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

Kumar

Srivastava

Mehta

et al. 2016

Journal of the Optical Society of Korea

View full text Add to dashboard Cite

show abstract

Beam propagation analysis on thickness measurements in quantitative phase microscopy

Cited by 1 publication

References 23 publications

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging

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