Beam deformation within an acousto-optic lens

Zhou, Zhenqiao; Li, Longhui; Wang, Jiancun; Hu, Qinglei; Zeng, Shaoqun

doi:10.1364/ol.40.002197

Cited by 8 publications

(1 citation statement)

References 12 publications

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“…In addition to dispersion, astigmatism also requires compensation when the fast-axis AOD operates in fast scanning mode, during which the AOD performs like a CYL. 32 The equivalent focal length of the CYL can be expressed as Eq. (5)

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Section: Methodsmentioning

confidence: 99%

Ten-kilohertz two-photon microscopy imaging of single-cell dendritic activity and hemodynamics in vivo

Wang

Lyu

et al. 2023

Neurophoton.

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. Significance The studying of rapid neuronal signaling across large spatial scales in intact, living brains requires both high temporal resolution and versatility of the measurement device. Aim We introduce a high-speed two-photon microscope based on a custom-built acousto-optic deflector (AOD). This microscope has a maximum line scan frequency of 400 kHz and a maximum frame rate of 10,000 frames per second (fps) at . For stepwise magnification from population view to subcellular view with high spatial and temporal resolution, we combined the AOD with resonance-galvo (RS) scanning. Approach With this combinatorial device that supports both large-view navigation and small-view high-speed imaging, we measured dendritic calcium propagation velocity and the velocity of single red blood cells (RBCs). Results We measured dendritic calcium propagation velocity ( ) in OGB-1-labeled single cortical neurons in mice in vivo . To benchmark the spatial precision and detection sensitivity of measurement in vivo , we also visualized the trajectories of single RBCs and found that their movement speed follows Poiseuille’s law of laminar flow. Conclusions This proof-of-concept methodological development shows that the combination of AOD and RS scanning two-photon microscopy provides both versatility and precision for quantitative analysis of single neuronal activities and hemodynamics in vivo .

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…”

Section: Methodsmentioning

confidence: 99%

Ten-kilohertz two-photon microscopy imaging of single-cell dendritic activity and hemodynamics in vivo

Wang

Lyu

et al. 2023

Neurophoton.

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A shared‐aperture S/X dual broadband microstrip antenna with one perforated patch

Kim

Hong

Kim

2019

Micro & Optical Tech Letters

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In this article, a novel dual broadband shared‐aperture perforated patch antenna is presented. The proposed antenna features dual broadband characteristics (S‐ and X‐bands) and relatively uncomplicated design consisting of a three‐layer structure without involving air gaps. The S‐band part of the antenna utilizes the aperture resonance and three‐section feed line to achieve a 26.5% bandwidth, while the X‐band part utilizes both aperture and stacked patch resonances to achieve a 25.6% bandwidth. The performance of the proposed dual broadband patch antenna is validated via numerical simulation and measurement.

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Monitoring the Oxygen Dynamics of Brain Tissue In Vivo by Fast Acousto-Optic Scanning Microscopy: A Proposed Instrument

Zhou

Chen

Huang

et al. 2016

Advances in Experimental Medicine and Biology

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The function of the brain neural circuit is highly dependent on oxygen supply. Imaging the precise oxygen distribution and dynamics are critical for understanding the relationship between neuronal activity and oxygen dynamics of the nearby capillaries. Here, we develop fast acousto-optic scanning two-photon microscopy. Combined with oxygen probes, such as PtP-C343, we can monitor oxygen dynamics at the submicron level by this real-time microscopy. In this fast acousto-optic scanning microscopy, an acousto-optic deflector (AOD), an inertia-less scanner, is used to scan the femtosecond laser. A cylindrical lens is used to compensate the 'cylindrical lens effect' of AOD and a prism is used to compensate the chromatic dispersion of AOD. An electro-optical modulator (EOM) and a sCMOS camera are gated to measure the phosphorescence lifetime. With a 40× water objective lens, this set-up can image a 100 μm × 100 μm field of view at a speed of 20 frames per second and a 25 μm × 8 μm field of view at a speed of 500 frames per second. This real-time two-photon microscopy is expected to be a good tool for observing and recording the precise rapid oxygen dynamics in the cerebral cortex, which will facilitate studies of oxygen metabolism in neurosciences.

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Beam deformation within an acousto-optic lens

Cited by 8 publications

References 12 publications

Ten-kilohertz two-photon microscopy imaging of single-cell dendritic activity and hemodynamics in vivo

Ten-kilohertz two-photon microscopy imaging of single-cell dendritic activity and hemodynamics in vivo

A shared‐aperture S/X dual broadband microstrip antenna with one perforated patch

Monitoring the Oxygen Dynamics of Brain Tissue In Vivo by Fast Acousto-Optic Scanning Microscopy: A Proposed Instrument

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