Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration

Cao, Yuanxiong; Tan, Jiayi; Zhao, Haoran; Deng, Ting; Hu, Yunxia; Zeng, Junhong; Li, Jiawei; Cheng, Yifan; Tang, Jiayu; Hu, Zhiwei; Hu, Keer; Xu, Bing; Wang, Zitian; Wu, Yaojiong; Lobie, Peter E.; Ma, Shaohua

doi:10.1038/s41467-022-35183-8

Cited by 30 publications

(19 citation statements)

References 60 publications

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“…We note that very recently a similar two-step method to encapsulate cells into gel beads and subsequently use 3D-deposition to print the gel beads into scaffolds has been published [33]. In this case mesenchymal stem cells (MSCs) were encapsulated into 450-650 µm beads consisting of gelatin, Matrigel, HAMA or GelMa, which were then deposited by air-jet assisted printing into stable 3Dscaffolds.…”

Section: In-flow Printing Of Alginate Microcapsule Constructsmentioning

confidence: 99%

On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process

Reineke,

Paulus,

Löffelsend

et al. 2024

Biofabrication

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Bioprinting has evolved into a thriving technology for the fabrication of cell-laden scaffolds. Bioinks are the most critical component for bioprinting. Recently, microgels have been introduced as a very promising bioink, enabling cell protection and the control of the cellular microenvironment. However, the fabrication of the bioinks involves the microfluidic production of the microgels, with a subsequent multistep process to obtain the bioink, which so far has limited its application potential. Here we introduce a direct coupling of microfluidics and 3D-printing for the continuous microfluidic production of microgels with direct in-flow printing into stable scaffolds. The 3D-channel design of the microfluidic chip provides access to different hydrodynamic microdroplet formation regimes to cover a broad range of droplet and microgel diameters. After exiting a microtubing the produced microgels are hydrodynamically jammed into thin microgel filaments for direct 3D-printing into two- and three-dimensional scaffolds. The methodology enables the continuous on-chip encapsulation of cells into monodisperse microdroplets with subsequent in-flow cross-linking to produce cell-laden microgels. The method is demonstrated for different cross-linking methods and cell lines. This advancement will enable a direct coupling of microfluidics and 3D-bioprinting for scaffold fabrication.

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Section: In-flow Printing Of Alginate Microcapsule Constructsmentioning

confidence: 99%

On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process

Reineke,

Paulus,

Löffelsend

et al. 2024

Biofabrication

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“…The 3D aspect of Imaris (and other similar software) has made it appealing for visualizing granular scaffolds, as well as computing VVF. [7,[11][12][13]…”

Section: Current Approaches For Imaging Granular Scaffolds and Comput...mentioning

confidence: 99%

Void Volume Fraction of Granular Scaffolds

Riley

Wei

Bao

et al. 2023

Small

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Void volume fraction (VVF) is a global measurement frequently used to characterize the void space of granular scaffolds, yet there is no gold standard by which to measure VVF in practice. To study the relationship between VVF and particles of varying size, form, and composition, a library of 3D simulated scaffolds is used. Results reveal that relative to particle count, VVF is a less predictable metric across replicate scaffolds. Simulated scaffolds are used to explores the relationship between microscope magnification and VVF, and recommendations are offered for optimizing the accuracy of approximating VVF using 2D microscope images. Lastly, VVF of hydrogel granular scaffolds is measured while varying four input parameters: image quality, magnification, analysis software, and intensity threshold. Results show that VVF is highly sensitive to these parameters. Overall, random packing produces variation in VVF among granular scaffolds comprising the same particle populations. Furthermore, while VVF is used to compare the porosity of granular materials within a study, VVF is a less reliable metric across studies that use different input parameters. VVF, a global measurement, cannot describe the dimensions of porosity within granular scaffolds, and the work supports the notion that more descriptors are necessary to sufficiently characterize void space.

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“…3D printing has emerged as a widely used technique to create biomimetic 3D scaffolds due to its ability to precisely control the spatial organization of materials (11)(12)(13). During 3D printing, gravity aids with material deposition but also adds a constant downward force on the printed objects (14), which can cause deformation or collapse of the printed objects, especially if overhanging or thin features are present.…”

Section: Introductionmentioning

confidence: 99%

Magnetically driven formation of 3D freestanding soft bioscaffolds

Xie,

Cao,

Sun

et al. 2024

Sci. Adv.

Self Cite

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3D soft bioscaffolds have great promise in tissue engineering, biohybrid robotics, and organ-on-a-chip engineering applications. Though emerging three-dimensional (3D) printing techniques offer versatility for assembling soft biomaterials, challenges persist in overcoming the deformation or collapse of delicate 3D structures during fabrication, especially for overhanging or thin features. This study introduces a magnet-assisted fabrication strategy that uses a magnetic field to trigger shape morphing and provide remote temporary support, enabling the straightforward creation of soft bioscaffolds with overhangs and thin-walled structures in 3D. We demonstrate the versatility and effectiveness of our strategy through the fabrication of bioscaffolds that replicate the complex 3D topology of branching vascular systems. Furthermore, we engineered hydrogel-based bioscaffolds to support biohybrid soft actuators capable of walking motion triggered by cardiomyocytes. This approach opens new possibilities for shaping hydrogel materials into complex 3D morphologies, which will further empower a broad range of biomedical applications.

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Bead-jet printing enabled sparse mesenchymal stem cell patterning augments skeletal muscle and hair follicle regeneration

Cited by 30 publications

References 60 publications

On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process

On-chip fabrication and in-flow 3D-printing of microgel constructs: from chip to scaffold materials in one integral process

Void Volume Fraction of Granular Scaffolds

Magnetically driven formation of 3D freestanding soft bioscaffolds

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