Bead-based microfluidic immunoassays: The next generation

Lim, Chwee Teck; Zhang, Y.

doi:10.1016/j.bios.2006.06.005

Cited by 249 publications

(101 citation statements)

References 55 publications

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“…They have, however, not been exploited at all in the microfluidics context. Recent modelling (Derksen, 2008(Derksen, , 2009) and experimental work (Doroodchi et al, 2012;Potic et al, 2005;Zivkovic et al, 2013a;Zivkovic et al, 2013b) has demonstrated that microfluidic fluidized beds (termed henceforth 'microfluidized beds') are feasible, offering the potential to not only overcome diffusion-limited heat and mass transport in simple micron-sized channels, but also provide higher sensitivity and multi-modal detection in the diagnostic context by virtue of the large surface area per unit volume that comes from use of micro-particles (Derveaux et al, 2008;Lim and Zhang, 2007).…”

Section: Introductionmentioning

confidence: 99%

On importance of surface forces in a microfluidic fluidized bed

Živković

Biggs

2015

Chemical Engineering Science

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Fluidized beds potentially offer a means of significantly enhancing mixing, heat and mass transfer under the low Reynolds number flow conditions that prevail in microfluidic devices. However, as surface forces at the microscale can be significant relative to hydrodynamics forces, fluidization within a microfluidic channel can be potentially hindered or even prevented through particle adhesion to the channel walls.We have used the acid-base theory of van Oss, Chaudhury and Good to predict the propensity for adhesion of particles on microfluidic fluidized bed walls for various practically important wall material/particle/fluid combinations. Comparison of the results from this approach with experimental observations indicates it provides a robust means of predicting the adhesion propensity. It is also demonstrated how results from the model can be used to estimate for a system of interest the particle size range in which the particle-wall surface forces transition from being dominate to being insignificant.

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Section: Introductionmentioning

confidence: 99%

On importance of surface forces in a microfluidic fluidized bed

Živković

Biggs

2015

Chemical Engineering Science

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“…56 The aggregation of Ab-MPs can form conveniently and rapidly; the total testing time (needed for both immunoaggregation and the detection) is approximately 1 h, which is faster than the conventional ELISA (requiring several hours to 2 days). 49 The sensor chip, made of PDMS and glass, is cost effective and portable, making it suitable for rapid, onsite, quantitative detection of multiple biomarkers. Recently, Hauer et al reported a two-stage device consisting of a tunable RPS to measure the size and concentration of microparticles, and fluorescence spectroscopy to detect the fluorescent labels simultaneously.…”

Section: Resultsmentioning

confidence: 99%

“…47,48 Recently, microparticle-enhanced immunoassays have attracted many attentions because surface functionalization of microparticles (MPs) is flexible and fast. 49,50 Among these microparticle-based immunoassays, immunoaggregation assays based on the aggregation of antibody functionalized MPs (Ab-MPs) triggered by target biomarkers enable direct measurement of a target biomarker concentration with only one step 51 without fluorescent, enzymatic, or radioactive labeling. However, traditional immunoaggregation detection method such as turbidimetry, nephelometry, and optical detection can only detect biomarkers with a relatively high concentration.…”

Section: Introductionmentioning

confidence: 99%

A multiplexed immunoaggregation biomarker assay using a two-stage micro resistive pulse sensor

Han

Liu

et al. 2016

Biomicrofluidics

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We present an immunoaggregation assay chip for multiplexed biomarkers detection. This chip is based on immunoaggregation of antibody functionalized microparticles (Ab-MPs) to quantify concentrations of multiple biomarkers simultaneously. A mixture of multiple types of Ab-MPs probes with different sizes and magnetic properties, which were functionalized by different antibodies, was used for the multiplexed assay. The interactions between biomarkers and their specific Ab-MPs probes caused the immunoaggregation of Ab-MPs. A two-stage micro resistive pulse sensor was used to differentiate and count the Ab-MP aggregates triggered by different biomarkers via size and magnetic property for multiplexed detection. The volume fraction of each type of Ab-MP aggregates indicates the concentration of the corresponding target biomarker. In our study, we demonstrated multiplexed detection of two model biomarkers (human ferritin and mouse anti-rabbit IgG) in 10% fetal bovine serum, using anti-ferritin Ab and anti-mouse IgG Ab functionalized MPs. We found that the volume fraction of Ab-MP aggregates increased with the increased biomarker concentrations. The detection ranges from 5.2 ng/ml to 208 ng/ml and 3.1 ng/ml to 5.12 Â 10 4 ng/ml were achieved for human ferritin and mouse anti-rabbit IgG. This bioassay chip is able to quantitatively detect multiple biomarkers in a single test without fluorescence or enzymatic labeling process and hence is promising to serve as a useful tool for rapid detection of multiple biomarkers in biomedical research and clinical applications. V C 2016 AIP Publishing LLC. [http://dx

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“…Particularly common is plate-based ELISA, in which antibodies are coated onto wells of a plate and incubated with clinical liquid samples. As a variation on plate-based ELISA, microbeads offer improved assay sensitivity and simplification of microfluidic device design [97,98]. In a typical microbead immunoassay, primary antibodies are bound to the beads, providing a larger surface area-to-volume ratio for antigen capture as compared to the plate format of ELISA; this increased surface area-to-volume ratio improves sample mixing efficiency and antigen capture.…”

Section: Antibody-bonded Substratesmentioning

confidence: 99%

Microdevices for Non-Invasive Detection of Bladder Cancer

et al. 2017

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Bladder cancer holds the record for the highest lifetime cost on a per-patient basis. This is due to high recurrence rates, which necessitate invasive and costly long-term evaluation methods such as cystoscopy and imaging. Microfluidics is emerging as an important approach to contribute to initial diagnosis and follow-up, by enabling the precise manipulation of biological samples. Specifically, microdevices have been used for the isolation of cells or genetic material from blood samples, sparking significant interest as a versatile platform for non-invasive bladder cancer detection with voided urine. In this review, we revisit the methods of bladder cancer detection and describe various types of markers currently used for evaluation. We detail cutting-edge technologies and evaluate their merits in the detection, screening, and diagnosis of bladder cancer. Advantages of microscale devices over standard methods of detection, as well as their limitations, are provided. We conclude with a discussion of criteria for guiding microdevice development that could deepen our understanding of prognoses at the level of individual patients and the underlying biology of bladder cancer development. Collectively, the development and widespread application of improved microfluidic devices for bladder cancer could drive treatment breakthroughs and establish widespread, tangible outcomes on patients' long-term survival.

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Bead-based microfluidic immunoassays: The next generation

Cited by 249 publications

References 55 publications

On importance of surface forces in a microfluidic fluidized bed

On importance of surface forces in a microfluidic fluidized bed

A multiplexed immunoaggregation biomarker assay using a two-stage micro resistive pulse sensor

Microdevices for Non-Invasive Detection of Bladder Cancer

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