BDNF-TrkB Axis Regulates Migration of the Lateral Line Primordium and Modulates the Maintenance of Mechanoreceptor Progenitors

Gasanov, Eugene V.; Rafieva, Lola M.; Korzh, Vladimir

doi:10.1371/journal.pone.0119711

Cited by 22 publications

(19 citation statements)

References 50 publications

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“…Brain derived neurotrophic factor ( bdnf ) is a transcription growth factor that is expressed throughout all life stages of the zebrafish, and has also been found to affect lateral line formation (Gasanov et al, 2015). Bdnf regulates the migration of the lateral line primordium, and enhances the differentiation of sensory and sympatic neurons (Diekmann et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Behavioral, morphometric, and gene expression effects in adult zebrafish (Danio rerio) embryonically exposed to PFOA, PFOS, and PFNA

2016

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Perfluoroalkylated substances (PFAS) are a class of persistent anthropogenic chemicals that have been detected worldwide. PFASs consist of fluorinated carbon chains of varying length, terminal groups, and have a number of industrial uses. A previous zebrafish study from our laboratory showed that acute (3–120 h post fertilization, 0.02–2.0μM), waterborne embryonic exposure to these chemicals resulted in chemical specific alterations at 5 days post fertilization (dpf), and some effects persisted up to 14 dpf. Using a gene battery consisting of 100 transcripts identified several genes that were up or down regulated. This current study looks at the long-term impacts of PFASs in adult zebrafish using the same exposure regimen. It was hypothesized that sub-lethal exposure of perfluorooctane sulfonate (PFOS), perfluorononanoic acid (PFNA), or perfluorooctane sulfonate (PFOA) in embryonic zebrafish (3–120 hpf) would result in permanent morphometric, gene expression, and behavioral changes in adult fish similar to those observed at 5 and 14 dpf. Zebrafish were exposed to PFOS, PFOA, and PFNA (Control 0μM, 2.0μM) for the first five days post fertilization. At six months post fertilization, no PFAS treatment resulted in a significant change in total body length or weight. In terms of behavior, PFNA males showed a reduction in total distance traveled and time of immobility, and an increase in thigmotaxis behavior, aggressive attacks, and preference for the bright section of the tank. PFOS treated males had a reduced aggression behavior, and PFOA females preferred the dark section of the tank. Gene expression of slco2b1, slco1d1, and tgfb1a were analyzed because these transcripts were previously found to be affected by PFAS exposure in 5dpf and 14 dpf zebrafish and resulted in: significant decrease in expression of slco2b1 for both sexes in PFNA and PFOS treated groups, significant decrease of slco1d1 in all treatment groups for females and PFOS and PFOA exposed males, significant increase of tgfb1a in males treated with PFOS and PFNA, and a significant increase of bdnf in all PFAS male groups. This study demonstrates that acute, embryonic exposure (5 days) to individual PFASs result in significant biochemical and behavioral changes in young adult zebrafish 6 months after exposure. These three PFASs have long term and persistent impacts following short term embryonic exposure that persists into adulthood.

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Section: Discussionmentioning

confidence: 99%

Behavioral, morphometric, and gene expression effects in adult zebrafish (Danio rerio) embryonically exposed to PFOA, PFOS, and PFNA

2016

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“…This conclusion also holds for the ntrk2 (neurotrophic receptor tyrosine kinase 2) gene. According to synteny, the ntrk2 gene has correctly named zebrafish ohnologs, 'a' and 'b' (Gasanov et al 2015).…”

Section: Zebrafish Ohnologs Using Syntenymentioning

confidence: 99%

Evolutionary context can clarify teleosts gene names

Gasanov

Jędrychowska

Kuźnicki

et al. 2020

Preprint

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SummaryThe initial convention to name genes relied on historical precedent, order in the human genome or mutants in model systems. However, partial duplication of genes in teleosts required naming the duplicated genes, so ohnologs adopted the ‘a’ or ‘b’ extension. Rapid advances in deciphering the zebrafish genome in relation to the human genome instituted naming genes in all other fish genomes in the convention of zebrafish. Unfortunately, some ohnologs and their resembling orthologs suffered from incorrect nomenclature, which created confusion in particular instances like establishing disease models. We sought to overcome this barrier by establishing the ex silico evolutionary-based systematic approach to naming the ohnologs in teleosts and other fish. We compared gene synteny using the spotted gar genome as the reference, which represents the unduplicated ancestral state. Using new criteria, we identified several hundreds of potentially misnamed ohnologs and validated manually several ohnologs as a proof pf principle. This may help to establish a standard naming practice resulting in the improved evolutionary-based gene nomenclature. This approach may help to identify and rename ohnologs in all relevant EMBL-EBI and NCBI databases starting from the zebrafish genome to avoid further proliferation of misleading information.

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“…6G',H',K). To examine whether transport of other neurotrophin receptors expressed in the pLLg, such as TrkB 38 , was disrupted, we analyzed trafficking of TrkB-mCherry fusion from embryos injected with neurod5kb:trkB-mCherry plasmid (Fig. 6I,J).…”

Section: Jip3 Mediates Retrograde Transport Of Activated Ret51 Receptormentioning

confidence: 99%

“…This suggests a defect in initiation of filopodial formation but not maintenance or continued growth. Failure to dynamically form filopodia could disrupt proper interaction with the extracellular environment, particularly the pLLp which is producing GDNF and BDNF 38 , necessary for guiding and promoting axon growth. One explanation for defects in axon outgrowth, then, may be that loss of filopodia leads to less interaction with the pLLp and thus a reduction of trophic/permissive cues sensed from the pLLp, further reducing the formation of growth cone filopodia and producing a positive feedback cycle that ends in failed growth cone advancement.…”

Section: Ret Signaling Regulates Pioneer Growth Cone Morphology and Dmentioning

confidence: 99%

A specific Ret receptor isoform is required for pioneer axon outgrowth and growth cone dynamics

et al. 2018

Preprint

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In many cases, axon growth and guidance are driven by pioneer axons, the first axons to grow in a particular region. Despite their dynamic pathfinding capabilities and developmental importance, there are very few pioneer neuron specific markers and thus their in vivo identification and functional interrogation have been difficult. We found that a Ret receptor isoform, Ret51, is highly enriched in peripheral sensory pioneer neurons and is required for pioneer axon outgrowth. Ret null mutant pioneer neurons differentiate normally; however, they displayed defects in growth cone morphology and formation of filopodia before pioneer axon extension prematurely halts. We also demonstrate loss-of-function of a retrograde cargo adaptor, JNK-interacting protein 3 (Jip3), phenocopied many of these axonal defects. We further found that loss of Jip3 led to accumulation of activated Ret receptor in pioneer growth cones, indicating a failure in the clearance of activated Ret from growth cones. Using an axon sever approach as well as in vivo analysis of axonal transport, we showed Jip3 specifically mediates retrograde, but not anterograde, transport of activated Ret51. Finally, live imaging revealed that Jip3 and Ret51 were retrogradely co-transported in pioneer axons, suggesting Jip3 functions as an adapter for retrograde transport of Ret51. Taken together, these results identify Ret51 as a molecular marker of pioneer neurons and elucidate an important isoform-specific role for Ret51 in axon growth and growth cone dynamics during development.

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BDNF-TrkB Axis Regulates Migration of the Lateral Line Primordium and Modulates the Maintenance of Mechanoreceptor Progenitors

Cited by 22 publications

References 50 publications

Behavioral, morphometric, and gene expression effects in adult zebrafish (Danio rerio) embryonically exposed to PFOA, PFOS, and PFNA

Behavioral, morphometric, and gene expression effects in adult zebrafish (Danio rerio) embryonically exposed to PFOA, PFOS, and PFNA

Evolutionary context can clarify teleosts gene names

A specific Ret receptor isoform is required for pioneer axon outgrowth and growth cone dynamics

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