BDNF Prevents and Reverses Adult Rat Motor Neuron Degeneration and Induces Axonal Outgrowth

Kishino, Akiyoshi; Ishige, Yohko; Tatsuno, Tohru; Nakayama, Chikao; Noguchi, Hiroshi

doi:10.1006/exnr.1996.6367

Cited by 191 publications

(115 citation statements)

References 39 publications

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“…BDNF, a ligand to TrkB and p75 NTR receptors (Squinto et al, 1991), has been reported to promote growth and enhance acetylcholine release from CNS neurons in rats (Auld et al, 2001;Kishino et al, 1997). However, treatment with BDNF did not have functional effects in the present experimental step-up.…”

Section: Discussioncontrasting

confidence: 55%

Prolonged exposure to NT-3 attenuates cholinergic nerve-mediated contractions in cultured murine airways

Bachar

Adner

Uddman

et al. 2005

Respiratory Physiology & Neurobiology

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Prolonged exposure to NT-3 attenuates cholinergic nerve-mediated contractions in cultured murine airways.Bachar, Ofir; Adner, Mikael; Uddman, Rolf; Cardell, Lars-Olaf General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal AbstractChronic airway inflammation may induce subsequent airway hyperresponsiveness (AHR) including pathological alteration of neural activity. Asthmatic airways contain elevated levels of neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) albeit; their effect on neural activity is unclear. This study evaluates the effects of NT-3 and BDNF on nerve mediated airway contractions in-vitro. Tracheal segments from BALB/c mice were cultured for 4 days with NT-3 or BDNF. Responsiveness to electric field stimulation (EFS) was evaluated in organ-bath and innervation patterns were examined by quantitative immunohistochemistry. In cultured segments the EFS induced contractions were inhibited by tetrodotoxin or atropine. NT-3 reduced the EFS contractions in a concentration-dependent manner whereas BDNF had no effect. The amount of nerve fibers, found in conjunction with the tracheal smooth muscle, was similar in NT-3 treated and control segments. In conclusion, NT-3 attenuates cholinergic nerve-mediated contractions of airway in-vitro. Considering the elevated levels of NT-3 found in asthmatic airways, the findings imply a protective role of NT-3 in AHR.

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Section: Discussioncontrasting

confidence: 55%

Prolonged exposure to NT-3 attenuates cholinergic nerve-mediated contractions in cultured murine airways

Bachar

Adner

Uddman

et al. 2005

Respiratory Physiology & Neurobiology

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“…It has been shown that, following nerve injury, BDNF does not increase functional recovery in tests such as sciatic function index, however, it has triggering effects. Although exogenous BDNF has inefficacies in postinjury functional recovering, endogenous BDNF is known to play an important role in peripheral nerve regeneration (38,55). Anti BDNF antibody application after damage has been reported both to diminish axonal elongation and to decrease the axonal density and number (56).…”

Section: Neurotrophinsmentioning

confidence: 99%

The role of growth factors in nerve regeneration

Önger

Delibaş

Türkmen

et al. 2016

DD&T

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“…NT-4/5 is the predominant neurotrophin expressed in the targets of mature facial and spinal motor neurons Funakoshi et al, 1995;Griesbeck et al, 1995). Adult motor neurons remain responsive to BDNF, because exogenously applied TrkB ligands maintain the cholinergic phenotype of axotomized adult facial, spinal, and hypoglossal motor neurons (Yan et al, 1994;Friedman et al, 1995;Tuszynski et al, 1996;Wang et al, 1997;Fernandes et al, 1998) and rescue avulsed motor neurons from injury-induced cell death Kishino et al, 1997;Novikov et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Adeno-Associated Viral Vector-Mediated Expression of Truncated TrkB in the Adult Rat Facial Nucleus Results in Motor Neuron Degeneration

et al. 2006

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Adult facial motor neurons continue to express full-length TrkB tyrosine kinase receptor (TrkB.FL), the high-affinity receptor for the neurotrophins BDNF and neurotrophic factor-4/5 (NT-4/5), suggesting that they remain dependent on target-derived and locally produced neurotrophins in adulthood. Studies on the role of TrkB signaling in the adult CNS have been hampered by the early lethality of bdnf, nt-4/5, and trkB knock-out mice. We disrupted TrkB.FL signaling in adult facial motor neurons using adeno-associated viral vector-mediated overexpression of a naturally occurring dominant-negative TrkB receptor, TrkB.T1. Expression of TrkB.T1 resulted in neuronal atrophy and downregulation of NeuN (neuronal-specific nuclear protein) and ChAT expression in facial motor neurons. A subset of transduced neurons displayed signs of motor neuron degeneration that included dendritic beading and rounding of the soma at 2 months of TrkB.T1 expression. Cell counts revealed a significant reduction in motor neuron number in the facial nucleus at 4 months after onset of expression of TrkB.T1, suggesting that a proportion of TrkB.T1-expressing motor neurons became undetectable as a result of severe atrophy or was lost because of cell death. In contrast, overexpression of TrkB.FL did not result in a decrease in facial motor neuron number. Our results indicate that a subset of facial motor neurons remains dependent on TrkB ligands for the maintenance of structural and molecular characteristics in adulthood.

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BDNF Prevents and Reverses Adult Rat Motor Neuron Degeneration and Induces Axonal Outgrowth

Cited by 191 publications

References 39 publications

Prolonged exposure to NT-3 attenuates cholinergic nerve-mediated contractions in cultured murine airways

Prolonged exposure to NT-3 attenuates cholinergic nerve-mediated contractions in cultured murine airways

The role of growth factors in nerve regeneration

Long-Term Adeno-Associated Viral Vector-Mediated Expression of Truncated TrkB in the Adult Rat Facial Nucleus Results in Motor Neuron Degeneration

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