BDNF expression with functional improvement in transected spinal cord treated with neural stem cells in adult rats

He, Bao-Li; Ba, Ying-Chun; Wang, Xuyang; Liu, Sujuan; Liu, Guodong; Ou, Shan; Gu, Yin-li; Pan, Xinghua; Wang, Ting‐Hua

doi:10.1016/j.npep.2012.06.001

Cited by 34 publications

(22 citation statements)

References 40 publications

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“…This result suggests that the increased levels of BDNF were possibly associated with the functional improvement by ASC intrathecal transplantation into the spinal cord in the rabbits. This result was supported by previous study that engrafted neural stem cells expressed BDNF and increased the level of BDNF mRNA in injured site following transplantation [32]. …”

Section: Discussionsupporting

confidence: 85%

Neuroprotective Effects of Adipose-Derived Stem Cells Are Maintained for 3 Weeks against Ischemic Damage in the Rabbit Spinal Cord

Moon

Kim

Chung

et al. 2014

BioMed Research International

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In the previous study, we demonstrated that adipose-derived stem cells (ASCs) have neuroprotective effects against ischemic damage in the ventral horn of L5-6 levels at 3 days after ischemia/reperfusion. In the present study, we expanded our observations for 3 weeks after ischemia/reperfusion to rule out the possibility of delayed neuronal death in several days after ischemia/reperfusion. Transient spinal cord ischemia was induced by a 15 min aortic artery occlusion in the subrenal region and rabbit ASCs were administered intrathecally into recipient rabbits (2 × 105) immediately after reperfusion. Transplantation of ASCs improved the neurological motor functions of the hindlimb 3 weeks after ischemia/reperfusion. Similarly, the cresyl violet-positive neurons were significantly increased at 3 weeks after ischemia/reperfusion compared to that in the vehicle (artificial cerebrospinal fluid)-treated group. The transplantation of ASCs significantly reduced reactive microglia induced by ischemia at 3 weeks after ischemia/reperfusion. In addition, transplantation of ASCs maintained the brain-derived neurotrophic factor (BDNF) levels 3 weeks after ischemia/reperfusion. These results suggest that the neuroprotective effects of ASCs are maintained 3 weeks after ischemia/reperfusion by modulating microgliosis and BDNF levels in the spinal cord.

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Section: Discussionsupporting

confidence: 85%

Neuroprotective Effects of Adipose-Derived Stem Cells Are Maintained for 3 Weeks against Ischemic Damage in the Rabbit Spinal Cord

Moon

Kim

Chung

et al. 2014

BioMed Research International

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“…Due to technical limitations, previous studies have usually focused on a small number of genes and pathways at a time [5,6,7,8,9,10,11,12,13,14,15], and thus did not provide a comprehensive view of the complex mechanisms contributing to SCI pathology. Considering the large number of biochemical cascades and cellular reactions involved, a global analysis is necessary in order to elucidate the pathophysiology of SCI.…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Characterization of Spinal Cord Injury Transcriptome in Acute/Subacute Phases: A Resource for Understanding the Pathology at the Systems Level

Chen¹,

Deng²,

Lü³

et al. 2013

PLoS ONE

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Spinal cord injury (SCI) is a devastating neurological disease without effective treatment. To generate a comprehensive view of the mechanisms involved in SCI pathology, we applied RNA-Sequencing (RNA-Seq) technology to characterize the temporal changes in global gene expression after contusive SCI in mice. We sequenced tissue samples from acute and subacute phases (2 days and 7 days after injury) and systematically characterized the transcriptomes with the goal of identifying pathways and genes critical in SCI pathology. The top enriched functional categories include “inflammation response,” “neurological disease,” “cell death and survival” and “nervous system development.” The top enriched pathways include LXR/RXR Activation and Atherosclerosis Signaling, etc. Furthermore, we developed a systems-based analysis framework in order to identify key determinants in the global gene networks of the acute and sub-acute phases. Some candidate genes that we identified have been shown to play important roles in SCI, which demonstrates the validity of our approach. There are also many genes whose functions in SCI have not been well studied and can be further investigated by future experiments. We have also incorporated pharmacogenomic information into our analyses. Among the genes identified, the ones with existing drug information can be readily tested in SCI animal models. Therefore, in this study we have described an example of how global gene profiling can be translated to identifying genes of interest for functional tests in the future and generating new hypotheses. Additionally, the RNA-Seq enables splicing isoform identification and the estimation of expression levels, thus providing useful information for increasing the specificity of drug design and reducing potential side effect. In summary, these results provide a valuable reference data resource for a better understanding of the SCI process in the acute and sub-acute phases.

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“…Not all axonal populations are responsive to BDNF, for example, Menei et al reported sensory and brainstem but no propriospinal regeneration (Menei et al, 1998). The situation with the impact of BDNF on sprouting/regrowth of corticospinal (CST) axons is not settled, with some reporting no effects (Brock et al, 2010;He et al, 2013;Iarikov et al, 2007;Lu et al, 2001;Schnell et al, 1994;Xu et al, 1995), while others described BDNF-induced sprouting of CST axons after SCI in adult rats (Lynskey et al, 2006;Sasaki et al, 2009;Vavrek et al, 2006). In most studies, exogenous BDNF increases myelination after SCI (Bamber et al, 2001;Golden et al, 2007;McTigue et al, 1998;Ollivier-Lanvin et al, 2014;Stokols et al, 2006;Xu et al, 1995;Zhao et al, 2013).…”

Section: Neuroprotection Sparing and Growth With Bdnfmentioning

confidence: 99%

“…Studies using thoracic SCI report either no additional improvements in locomotor function over controls (Koda et al, 2004(Koda et al, , 2007Lu et al, 2005;Sharp et al, 2012;Vavrek et al, 2006) or partial and/or significant improvements Boyce et al, 2012;Coumans et al, 2001;Feng et al, 2009;Han et al, 2014;He et al, 2013;Houweling, van Asseldonk, et al, 1998;Lu et al, 2012;Ollivier-Lanvin et al, 2014;Sasaki et al, 2009;Ziemlinska et al, 2014). Hains et al reported reduced allodynia/mechanical responses that may in part be attributed to BDNF (Hains et al, 2003).…”

Section: Functional Recovery With Bdnfmentioning

confidence: 99%

Neurotrophic Factors Used to Treat Spinal Cord Injury

Hodgetts

Harvey

2017

Vitamins and Hormones

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BDNF expression with functional improvement in transected spinal cord treated with neural stem cells in adult rats

Cited by 34 publications

References 40 publications

Neuroprotective Effects of Adipose-Derived Stem Cells Are Maintained for 3 Weeks against Ischemic Damage in the Rabbit Spinal Cord

Neuroprotective Effects of Adipose-Derived Stem Cells Are Maintained for 3 Weeks against Ischemic Damage in the Rabbit Spinal Cord

RNA-Seq Characterization of Spinal Cord Injury Transcriptome in Acute/Subacute Phases: A Resource for Understanding the Pathology at the Systems Level

Neurotrophic Factors Used to Treat Spinal Cord Injury

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