BDNF and NT3 extend the critical period for developmental climbing fibre plasticity

Sherrard, Rachel M.; Bower, Adrian J.

doi:10.1097/00001756-200109170-00023

Cited by 28 publications

(33 citation statements)

References 24 publications

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“…When either BDNF or NT-3 was applied to the whisker pads following neonatal denervation, barrel patterns developed in a manner more consistent with normal development (Calia et al, 1998). In another study, reinnervation of cerebellum occurred if climbing fibers were transected prior to postnatal day 7 in the rat, but reinnervation occurred only after the addition of exogenous BDNF or NT-3 if transection was given at later ages (Sherrard and Bower, 2001). Such studies not only lend further support for the role of neurotrophic regulation following denervation during developmental sensitive periods, but also provide impetus to determine if exogenous application of neurotrophins following neonatal CTX might result in maintenance of papillae and taste buds.…”

Section: Discussionmentioning

confidence: 92%

Chorda tympani nerve transection at different developmental ages produces differential effects on taste bud volume and papillae morphology in the rat

Sollars

2005

J. Neurobiol.

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Chorda tympani nerve transection (CTX) results in morphological changes to fungiform papillae and associated taste buds. When transection occurs during neonatal development in the rat, the effects on fungiform taste bud and papillae structure are markedly more severe than observed following a comparable surgery in the adult rat. The present study examined the potential "sensitive period" for morphological modifications to tongue epithelium following CTX. Rats received unilateral transection at 65, 30, 25, 20, 15, 10, or 5 days of age. With each descending age at the time of transection, the effects on the structural integrity of fungiform papillae were more severe. Significant losses in total number of taste buds and filiform-like papillae were observed when transection occurred 5-30 days of age. Significant reduction in the number of taste pores was indicated at every age of transection. Another group of rats received chorda tympani transection at 10, 25, or 65 days of age to determine if the time course of taste bud degeneration differed depending on the age of the rat at the time of transection. Taste bud volumes differed significantly from intact sides of the tongue at 2, 8, and 50 days post-transection after CTX at 65 days of age. Volume measurements did not differ 2 days post-transection after CTX at 10 or 25 days of age, but were significantly reduced at the other time points. Findings demonstrate a transitional period throughout development wherein fungiform papillae are highly dependent upon the chorda tympani for maintenance of morphological integrity.

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Section: Discussionmentioning

confidence: 92%

Chorda tympani nerve transection at different developmental ages produces differential effects on taste bud volume and papillae morphology in the rat

Sollars

2005

J. Neurobiol.

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“…Moreover, data obtained in experimental animals indicate that NTs may have a role in repair of cerebellar neurons and connectivity. In fact, it has been reported that the BDNF and NT-4 rescue cerebellar granule neurons from oxidative stress-mediated apopotic death (Skaper et al, 1998) and several lines of evidence brought to attention the trophic role of NTs on cerebellar afferent neurons (Shibayama et al, 1998;Rabacchi et al, 1999;Sherrard and Bower, 2001). Positive structures for each NT were observed in all specimens examined.…”

Section: Nts Tissue Localizationmentioning

confidence: 82%

Neurotrophin‐like immunoreactivity in the human pre‐term newborn, infant, and adult cerebellum

Quartu

Serra

Manca

et al. 2003

Intl J of Devlp Neuroscience

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The immunohistochemical occurrence of the neurotrophin (NT) proteins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-4 (NT-4), and neurotrophin-3 (NT-3) is shown in the pre-term newborn, infant, and adult human post-mortem cerebellum. The NT-like immunoreactive structures were unevenly distributed and showed regional differences among cerebellar lobules and folia. NGF-, NT-4-, and NT-3-positive neuronal perikarya were observed in all specimens examined. At variance with the other neurotrophins, the BDNF antiserum labelled neuronal cell bodies only in newborn life and infancy, as well as extensive nerve fibre systems, whose density increased with age. The NT-antibodies, tested by Western blot on human cerebellum homogenates, revealed immunoreactive bands corresponding to proteins of heterogenous molecular weight. The results obtained provide a first demonstration of the tissue localization of the NTs in the human cerebellum from perinatal to adult age, thus suggesting their involvement in the development, differentiation and maintenance of the cerebellar connectivity. Codistribution of the four NTs or sets of them was observed in cortical and deep nuclei neurons. Multiple trophic roles for NTs, encompassing the classic target-derived and local mechanisms of support, are envisaged as significant in development, differentiation, and maintenance of the human cerebellar connectivity.

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“…We also investigated the effects of LI-rMS on the expression of four candidate genes, which are involved in olivo-cerebellar development and plasticity (Morrison and Mason, 1998; Sherrard and Bower, 2001; Avella et al, 2006; Bosman et al, 2006; Zhang et al, 2007; Sherrard et al, 2009; Sherrard et al, 2013). Although, it has been shown that magnetic stimulation alters gene expression in different neuronal populations in vivo and in vitro (high intensity: Funke and Benali, 2011; Stock et al, 2012; Vlachos et al, 2012; Ma et al, 2013, low intensity: Rodger et al, 2012; Makowiecki et al, 2014; Grehl et al, 2015) we show for the first time stimulation-related effects of LI-rMS on cerebellar and inferior olive tissue.…”

Section: Discussionmentioning

confidence: 99%

In vitro Magnetic Stimulation: A Simple Stimulation Device to Deliver Defined Low Intensity Electromagnetic Fields

Grehl

Martina

Goyenvalle

et al. 2016

Front. Neural Circuits

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Non-invasive brain stimulation (NIBS) by electromagnetic fields appears to benefit human neurological and psychiatric conditions, although the optimal stimulation parameters and underlying mechanisms remain unclear. Although, in vitro studies have begun to elucidate cellular mechanisms, stimulation is delivered by a range of coils (from commercially available human stimulation coils to laboratory-built circuits) so that the electromagnetic fields induced within the tissue to produce the reported effects are ill-defined. Here, we develop a simple in vitro stimulation device with plug-and-play features that allow delivery of a range of stimulation parameters. We chose to test low intensity repetitive magnetic stimulation (LI-rMS) delivered at three frequencies to hindbrain explant cultures containing the olivocerebellar pathway. We used computational modeling to define the parameters of a stimulation circuit and coil that deliver a unidirectional homogeneous magnetic field of known intensity and direction, and therefore a predictable electric field, to the target. We built the coil to be compatible with culture requirements: stimulation within an incubator; a flat surface allowing consistent position and magnetic field direction; location outside the culture plate to maintain sterility and no heating or vibration. Measurements at the explant confirmed the induced magnetic field was homogenous and matched the simulation results. To validate our system we investigated biological effects following LI-rMS at 1 Hz, 10 Hz and biomimetic high frequency, which we have previously shown induces neural circuit reorganization. We found that gene expression was modified by LI-rMS in a frequency-related manner. Four hours after a single 10-min stimulation session, the number of c-fos positive cells increased, indicating that our stimulation activated the tissue. Also, after 14 days of LI-rMS, the expression of genes normally present in the tissue was differentially modified according to the stimulation delivered. Thus we describe a simple magnetic stimulation device that delivers defined stimulation parameters to different neural systems in vitro. Such devices are essential to further understanding of the fundamental effects of magnetic stimulation on biological tissue and optimize therapeutic application of human NIBS.

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BDNF and NT3 extend the critical period for developmental climbing fibre plasticity

Cited by 28 publications

References 24 publications

Chorda tympani nerve transection at different developmental ages produces differential effects on taste bud volume and papillae morphology in the rat

Chorda tympani nerve transection at different developmental ages produces differential effects on taste bud volume and papillae morphology in the rat

Neurotrophin‐like immunoreactivity in the human pre‐term newborn, infant, and adult cerebellum

In vitro Magnetic Stimulation: A Simple Stimulation Device to Deliver Defined Low Intensity Electromagnetic Fields

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